Difference between revisions of "Team:Northwestern/09 16"

 
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Latest revision as of 15:36, 18 October 2016

Notebook

Friday, September 16th

Tasks:

Jordan

  • Went to Auburn Gresham for outreach

Sara

  • Went to Auburn Gresham for outreach

Tasfia

  • Presented at Auburn Gresham
  • Collected 8 primers from Leonard lab office
  • Ran PCR for linearization of pET28a plasmid miniprep (one 50-μL reaction)
    • 0.5 μL pet28 miniprep (23 ng/μL)
    • 2.5 μL 10μM Lnrz_pET28a_FWD
    • 2.5 μL 10 μM Lnrz_pET28a_REV
    • 1 μL DMSO
    • 18.5 μL nuclease-free water
    • 25 μL Q5 HiFi 2X Master Mix
  • Ran PCR for amplifying Cas9 to insert to Pet28 backbone (one 50-μL reaction)
    • 1 μL Cas9 1:3 template
    • 1 μL 10 μM Cas9_pET28a_insertFWD
    • 1 μL 10 μM Cas9-pET28a_insertREV
    • 1 μL DMSO
    • 21 μL nuclease-free water
    • 25 μL OneTaq 2X Master Mix

Tyler

  • Went to Auburn Gresham for outreach