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         <h2 class="h2">Section 1</h2>
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         <h2 class="h2">Introduction</h2>
 
              
 
              
 
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                Has ut facer debitis, quo eu agam purto. In eum justo aeterno. Sea ut atqui efficiantur, mandamus deseruisse at est, erat natum cum eu. Quot numquam in vel. Salutatus euripidis moderatius qui ex, eu tempor volumus vituperatoribus has, ius ea ullum facer corrumpit.
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By developing tools to genetically engineer Yarrowia lipolytica we aim to create a versatile
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cell factory, which in the future will be able to produce almost any desired product ranging
 +
 
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from complex therapeutic proteins to high value chemical compounds. Besides diversity of
 +
 
 +
products cell factory based on Yarrowia lipolytica offers a wide range of substrate tolerance.
 +
 
 +
We aim to demonstrate this versatility of our Y. lipolytica strain as a cell factory by
 +
 
 +
producing an extracellular heterologous protein such as proinsulin. Moreover to prove
 +
 
 +
compliance of our cell factory with the iGEM BioBricks standards we aim to produce beta-
 +
 
 +
carotene from the registered BioBricks. In order to introduce genes necessary to achieve
 +
 
 +
both of mentioned ideas we apply pSB1A8yl plasmid, constructed by our team.
 
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Introduction

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By developing tools to genetically engineer Yarrowia lipolytica we aim to create a versatile cell factory, which in the future will be able to produce almost any desired product ranging from complex therapeutic proteins to high value chemical compounds. Besides diversity of products cell factory based on Yarrowia lipolytica offers a wide range of substrate tolerance. We aim to demonstrate this versatility of our Y. lipolytica strain as a cell factory by producing an extracellular heterologous protein such as proinsulin. Moreover to prove compliance of our cell factory with the iGEM BioBricks standards we aim to produce beta- carotene from the registered BioBricks. In order to introduce genes necessary to achieve both of mentioned ideas we apply pSB1A8yl plasmid, constructed by our team.

Section 2

Has ut facer debitis, quo eu agam purto. In eum justo aeterno. Sea ut atqui efficiantur, mandamus deseruisse at est, erat natum cum eu. Quot numquam in vel. Salutatus euripidis moderatius qui ex, eu tempor volumus vituperatoribus has, ius ea ullum facer corrumpit.

Section 2.1

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Section 2.2

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Section 2.3

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Section 3

Has ut facer debitis, quo eu agam purto. In eum justo aeterno. Sea ut atqui efficiantur, mandamus deseruisse at est, erat natum cum eu. Quot numquam in vel. Salutatus euripidis moderatius qui ex, eu tempor volumus vituperatoribus has, ius ea ullum facer corrumpit.

Section 4

Has ut facer debitis, quo eu agam purto. In eum justo aeterno. Sea ut atqui efficiantur, mandamus deseruisse at est, erat natum cum eu. Quot numquam in vel. Salutatus euripidis moderatius qui ex, eu tempor volumus vituperatoribus has, ius ea ullum facer corrumpit.

Section 5

Has ut facer debitis, quo eu agam purto. In eum justo aeterno. Sea ut atqui efficiantur, mandamus deseruisse at est, erat natum cum eu. Quot numquam in vel. Salutatus euripidis moderatius qui ex, eu tempor volumus vituperatoribus has, ius ea ullum facer corrumpit.

Section 6

Has ut facer debitis, quo eu agam purto. In eum justo aeterno. Sea ut atqui efficiantur, mandamus deseruisse at est, erat natum cum eu. Quot numquam in vel. Salutatus euripidis moderatius qui ex, eu tempor volumus vituperatoribus has, ius ea ullum facer corrumpit.

Section 7

Has ut facer debitis, quo eu agam purto. In eum justo aeterno. Sea ut atqui efficiantur, mandamus deseruisse at est, erat natum cum eu. Quot numquam in vel. Salutatus euripidis moderatius qui ex, eu tempor volumus vituperatoribus has, ius ea ullum facer corrumpit.

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