Difference between revisions of "Team:UPMC-Paris/Material"

Line 4: Line 4:
 
<div id="content2">
 
<div id="content2">
 
<div id="text">
 
<div id="text">
−
<p>
+
<p><br>
−
<b><u>Kits</b></u><br>
+
<b><u>Kits</b></u></p>
 
<br>
 
<br>
−
.Monarch Plasmid Miniprep Kit, From NEB Ref: #1010TS
+
<ol>
 +
<li>.Monarch Plasmid Miniprep Kit from NEB Ref: T1010S</li>
 +
<li>.Monarch PCR & DNA Cleanup Kit (5ug) Ref: T1030S</li>
 +
<li>.Phusion High-Fidelity PCR Kit from NEB Ref: E0553S</li>
 +
<li>.QiAquick Gel Extraction Kit from Qiagen Ref: 28704</li>
 +
<li>.QiAquick PCR Purification Kit from Qiagen Ref: 28104</li>
  
 +
</ol>
 +
<p><br>
 +
<b><u>Bacterial Strains</b></u></p>
 +
<p><br>
 +
You can find our bacteria <a href="https://2016.igem.org/Team:UPMC-Paris/Safety">here</a>!
 
</p>
 
</p>
 +
 +
<p><br>
 +
<b><u>Media and chemicals</b></u></p>
 +
<p><br>
 +
.LB Broth-Miller from Difco, Ref: 244620<br>
 +
.Bacto Yeast Extract from Difco, Ref: 212750<br>
 +
.Quick-Load 1 kb DNA Ladder from NEB, Ref: N0468S<br>
 +
.Formic Acid Free Acid from Sigma-Aldrich, Ref: F0507-100ML-D<br>
 +
.Manganese(II) Sulfate Monohydrate from Sigma-Aldrich, Ref: M7634-100G-D<br>
 +
.Zinc Sulfate Hepthydrate from Sigma-Aldrich, Ref: Z4750-100G-D<br>
 +
.Ferric Chloride Hexahydrate from Sigma-Aldrich, Ref: F2877-100G-D<br>
 +
.Thiamine Hydrochloride from Sigma-Aldrich, Ref: T4625-5G<br>
 +
.Chelex 100 Sodium Form (Chelating Resin) from Sigma-Aldrich, Ref: C7901-25G<br>
 +
.<br>
 +
.Nicotinic Acid 99% from Grosseron, Ref: A12683.30<br>
 +
.Phusion High-Fidelity PCR Master Mix with HF Buffer from Thermo Fisher Scientific, Ref: F531S<br>
 +
.
 +
<p><br>
 +
<b><u>Enzymes</b></u></p>
 +
<p><br>
 +
.Pvu II, recombinant from NEB, Ref: R0151S<br>
 +
.Avr II, recombinant from NEB, Ref: R0174S<br>
 +
.Pac I, recombinant from NEB, Ref: R0547S<br>
 +
.Psc I from Thermo Fisher Scientific, Ref: ER1871<br>
 +
.Kpn I from Thermo FIsher Scientific, Ref: ER0521<br>
 +
.<br>
 +
.<br>
 +
.<br>
 +
.<br>
 +
.<br>
 +
.<br>
 +
.T4 DNA Ligase from Sigma-Aldrich, Ref: 10481220001<br>
 +
 +
<p><br>
 +
<b><u>Antibiotics</b></u></p>
 +
<p><br>
 +
.Cloramphenicol from Sigma-Aldrich, Ref: C0378-5G<br>
 +
.Kanamycine sulfate from Termo Fisher Scientific, Ref: 11815024<br>
 +
 +
<p><br>
 +
<b><u>DNA and Plasmids</b></u></p>
 +
<p><br>
 +
Our PCR primers were synthesized by Eurofins<br>
 +
Our Biobricks fragments were synthesized by IDT<br>
 +
 
<table>
 
<table>
 
   <tr>
 
   <tr>

Revision as of 04:20, 19 October 2016