Difference between revisions of "Team:TAS Taipei/Modeling"

(Created page with " <html> <head> <title>TAS Taipei iGEM Wiki</title> <link rel="shortcut icon" href="https://static.igem.org/mediawiki/2014hs/2/2f/Tas_igemlogo.png"> <link href='http://fonts.googl...")
 
Line 1: Line 1:
  
−
<html>
+
<!DOCTYPE html>
 +
<html lang="en" dir="ltr" class="client-nojs">
 +
<head>
 +
   
 +
    <meta charset="UTF-8" />
 +
    <title>Team:TAS Taipei - 2016.igem.org</title>
 +
    <meta name="generator" content="MediaWiki 1.24.1" />
 +
    <link rel="shortcut icon" href="/favicon.ico" />
 +
    <link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2015.igem.org (en)" />
 +
    <link rel="EditURI" type="application/rsd+xml" href="https://2015.igem.org/wiki/api.php?action=rsd" />
 +
    <link rel="alternate" hreflang="x-default" href="/Team:TAS_Taipei" />
 +
    <link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
 +
    <link rel="alternate" type="application/atom+xml" title="2015.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" />
 +
    <link rel="stylesheet" href="https://2016.igem.org/wiki/load.php?debug=false&amp;lang=en&amp;modules=mediawiki.legacy.commonPrint%2Cshared%7Cmediawiki.skinning.content.externallinks%7Cmediawiki.skinning.interface%7Cmediawiki.ui.button%7Cskins.igem.styles&amp;only=styles&amp;skin=igem&amp;*" />
 +
<!--[if IE 6]><link rel="stylesheet" href="/wiki/skins/Igem/IE60Fixes.css?303" media="screen" /><![endif]-->
 +
<!--[if IE 7]><link rel="stylesheet" href="/wiki/skins/Igem/IE70Fixes.css?303" media="screen" /><![endif]-->
 +
    <meta name="ResourceLoaderDynamicStyles" content="" />
 +
    <style>a:lang(ar),a:lang(kk-arab),a:lang(mzn),a:lang(ps),a:lang(ur){text-decoration:none}
 +
        /* cache key: 2015_igem_org:resourceloader:filter:minify-css:7:875d2068ffef0b9ce1dbab8400ce2379 */</style>
 +
    <script src="https://2015.igem.org/wiki/load.php?debug=false&amp;lang=en&amp;modules=startup&amp;only=scripts&amp;skin=igem&amp;*"></script>
 +
    <script>if(window.mw){
 +
            mw.config.set({"wgCanonicalNamespace":"","wgCanonicalSpecialPageName":false,"wgNamespaceNumber":0,"wgPageName":"Team:TAS_Taipei","wgTitle":"Team:TAS Taipei","wgCurRevisionId":399114,"wgRevisionId":399114,"wgArticleId":1828,"wgIsArticle":true,"wgIsRedirect":false,"wgAction":"view","wgUserName":null,"wgUserGroups":["*"],"wgCategories":[],"wgBreakFrames":false,"wgPageContentLanguage":"en","wgPageContentModel":"wikitext","wgSeparatorTransformTable":["",""],"wgDigitTransformTable":["",""],"wgDefaultDateFormat":"dmy","wgMonthNames":["","January","February","March","April","May","June","July","August","September","October","November","December"],"wgMonthNamesShort":["","Jan","Feb","Mar","Apr","May","Jun","Jul","Aug","Sep","Oct","Nov","Dec"],"wgRelevantPageName":"Team:TAS_Taipei","wgIsProbablyEditable":false,"wgRestrictionEdit":[],"wgRestrictionMove":[],"wgWikiEditorEnabledModules":{"toolbar":false,"dialogs":false,"hidesig":true,"preview":false,"previewDialog":false,"publish":false}});
 +
            }</script>
 +
    <script>if(window.mw){
 +
        mw.loader.implement("user.options",function($,jQuery){mw.user.options.set({"ccmeonemails":0,"cols":80,"date":"default","diffonly":0,"disablemail":0,"editfont":"default","editondblclick":0,"editsectiononrightclick":0,"enotifminoredits":0,"enotifrevealaddr":0,"enotifusertalkpages":1,"enotifwatchlistpages":1,"extendwatchlist":0,"fancysig":0,"forceeditsummary":0,"gender":"unknown","hideminor":0,"hidepatrolled":0,"imagesize":2,"math":1,"minordefault":0,"newpageshidepatrolled":0,"nickname":"","norollbackdiff":0,"numberheadings":0,"previewonfirst":0,"previewontop":1,"rcdays":7,"rclimit":50,"rows":25,"showhiddencats":0,"shownumberswatching":1,"showtoolbar":1,"skin":"igem","stubthreshold":0,"thumbsize":5,"underline":2,"uselivepreview":0,"usenewrc":1,"watchcreations":1,"watchdefault":1,"watchdeletion":0,"watchlistdays":3,"watchlisthideanons":0,"watchlisthidebots":0,"watchlisthideliu":0,"watchlisthideminor":0,"watchlisthideown":0,"watchlisthidepatrolled":0,"watchmoves":0,"watchrollback":0,
 +
      "wllimit":250,"useeditwarning":1,"prefershttps":1,"language":"en","variant-gan":"gan","variant-iu":"iu","variant-kk":"kk","variant-ku":"ku","variant-shi":"shi","variant-sr":"sr","variant-tg":"tg","variant-uz":"uz","variant-zh":"zh","searchNs0":true,"searchNs1":false,"searchNs2":false,"searchNs3":false,"searchNs4":false,"searchNs5":false,"searchNs6":false,"searchNs7":false,"searchNs8":false,"searchNs9":false,"searchNs10":false,"searchNs11":false,"searchNs12":false,"searchNs13":false,"searchNs14":false,"searchNs15":false,"variant":"en"});},{},{});mw.loader.implement("user.tokens",function($,jQuery){mw.user.tokens.set({"editToken":"+\\","patrolToken":"+\\","watchToken":"+\\"});},{},{});
 +
/* cache key: 2015_igem_org:resourceloader:filter:minify-js:7:c2a6e0f393444edcfe542f485c3692c2 */
 +
}</script>
 +
    <script>if(window.mw){
 +
            mw.loader.load(["mediawiki.page.startup","mediawiki.legacy.wikibits","mediawiki.legacy.ajax"]);
 +
        }</script>
 +
   
 +
   
 +
</head>
 +
   
 +
<body class="mediawiki ltr sitedir-ltr ns-0 ns-subject page-Team_TAS_Taipei skin-igem action-view">
 +
 
 +
        <script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
 +
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
 +
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
 +
 
 +
    <div id='globalWrapper'>
 +
        <div id='top_menu_under' class='noprint'></div>
 +
        <div id='top_menu_14' class='noprint'>Loading menubar.....</div> <!-- Will be replaced with the jQuery.load -->
 +
<script>jQuery('#top_menu_14').load('https://2015.igem.org/cgi/top_menu_14/menubar_reply.cgi',
 +
    {  t:"Team%3ATAS%20Taipei",
 +
a:"View+%2FTeam%3ATAS_Taipei++View source+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3ATAS_Taipei%26action%3Dedit++History+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3ATAS_Taipei%26action%3Dhistory++Page+%2FTeam%3ATAS_Taipei++Discussion+%2Fwiki%2Findex.php%3Ftitle%3DTalk%3ATeam%3ATAS_Taipei%26action%3Dedit%26redlink%3D1++" });
 +
 +
</script>
 +
       
 +
<div id="content" class="mw-body" role="main">
 +
    <a id="top"></a>
 +
    <h1 id="firstHeading" class="firstHeading">
 +
        <span dir="auto">Team:TAS Taipei</span>
 +
    </h1>
 +
    <div id="bodyContent">
 +
<div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><p>
  
 
<head>
 
<head>
Line 6: Line 62:
 
<link rel="shortcut icon" href="https://static.igem.org/mediawiki/2014hs/2/2f/Tas_igemlogo.png">
 
<link rel="shortcut icon" href="https://static.igem.org/mediawiki/2014hs/2/2f/Tas_igemlogo.png">
 
<link href='http://fonts.googleapis.com/css?family=Lato' rel='stylesheet' type='text/css'>
 
<link href='http://fonts.googleapis.com/css?family=Lato' rel='stylesheet' type='text/css'>
−
<link rel="stylesheet" href="https://2015.igem.org/Template:TAS_Taipei/css/compiled?action=raw&ctype=text/css">
+
<link rel="stylesheet" href="page1.css">
−
<link rel="stylesheet" href="https://2015.igem.org/Template:TAS_Taipei/css/bootstrap?action=raw&ctype=text/css">
+
<link rel="stylesheet" href="page1b.css">
  
 
<script>localStorage.clear();</script>
 
<script>localStorage.clear();</script>
−
<script src="https://2015.igem.org/Template:TAS_Taipei/js/jquery?action=raw&ctype=text/javascript"></script>
+
<script src="https://2015.igem.org/Template:TAS_Taipei/js/jquery?action=raw&amp;ctype=text/javascript"></script>
−
<script src='https://2015.igem.org/Template:TAS_Taipei/js/bootstrap?action=raw&ctype=text/javascript'></script>
+
<script src='https://2015.igem.org/Template:TAS_Taipei/js/bootstrap?action=raw&amp;ctype=text/javascript'></script>
−
<script src="https://2015.igem.org/Template:TAS_Taipei/js/jquerySlides?action=raw&ctype=text/javascript"></script>
+
<script src="https://2015.igem.org/Template:TAS_Taipei/js/jquerySlides?action=raw&amp;ctype=text/javascript"></script>
  
  
Line 46: Line 102:
 
</div>
 
</div>
 
<div>
 
<div>
−
<h5><a href="https://2015.igem.org/Team:TAS_Taipei/Parts">Parts</a></h5>
+
<h5><a href="https://2015.igem.org/Team:TAS_Taipei/Parts">FUN!</a></h5>
 
</div>
 
</div>
 
</div>
 
</div>
 
<div style='height:140px;'>
 
<div style='height:140px;'>
 
<img src="https://static.igem.org/mediawiki/2015/5/5b/Tas_icon_project.png">
 
<img src="https://static.igem.org/mediawiki/2015/5/5b/Tas_icon_project.png">
−
<h4><b>Granzyme B</b> - a blessing and a curse. It's crucial for controlling cancer, but it causes damage when out of cellular space. Let's find the best of both worlds with <b>selective GzmB Inhibition.</b></h4>
+
<h4><b>GSH</b> - the solution to cataracts. How can we <b>increase</b> it in our eyes? </h4>
 
</div>
 
</div>
 
 
Line 177: Line 233:
 
<br>
 
<br>
 
<br>
 
<br>
−
<div class="row center-block" style = "padding:10px;width:960px;background-color:#FFC107;box-shadow:0px 0px 5px black">
+
<div class="row center-block" style = "padding:10px;width:960px;background-color:#3321FB;box-shadow:0px 0px 5px black">
 
<div class="row" style = "text-align:center;">
 
<div class="row" style = "text-align:center;">
 
<div class="col-sm-2">
 
<div class="col-sm-2">
−
<a href="https://2015.igem.org/Team:TAS_Taipei" style='text-decoration: none'><img src="https://static.igem.org/mediawiki/2015/2/2f/Tas_igemlogo.png
+
<a href="https://2016.igem.org/Team:TAS_Taipei" style='text-decoration: none'><img src="https://static.igem.org/mediawiki/2015/2/2f/Tas_igemlogo.png
 
" alt="" style="width: 100px;"></a>
 
" alt="" style="width: 100px;"></a>
 
</div>
 
</div>
 
<div class="col-sm-8" style='text-align:center;'>
 
<div class="col-sm-8" style='text-align:center;'>
−
<a href="https://2015.igem.org/Team:TAS_Taipei" style='text-decoration: none;'>
+
<a href="https://2016.igem.org/Team:TAS_Taipei" style='text-decoration: none;'>
 
<h2 style="font-family:'Lato';letter-spacing:10px;color: white; font-size: 60px;  margin-top: 0;  margin-bottom: 0;"><b>
 
<h2 style="font-family:'Lato';letter-spacing:10px;color: white; font-size: 60px;  margin-top: 0;  margin-bottom: 0;"><b>
−
<img style = "height:64px;vertical-align:top" src = "https://static.igem.org/mediawiki/2015/5/5b/Tas_icon_project.png">RANZYME B</b></h2>
+
CATARACTS</b></h2>
 
</a>
 
</a>
 
</div>
 
</div>
Line 195: Line 251:
 
</div>
 
</div>
  
−
<script type="text/javascript" src="https://2015.igem.org/Template:TAS_Taipei/js/jssor?action=raw&ctype=text/javascript"></script>
+
<script type="text/javascript" src="https://2015.igem.org/Template:TAS_Taipei/js/jssor?action=raw&amp;ctype=text/javascript"></script>
−
<script type="text/javascript" src="https://2015.igem.org/Template:TAS_Taipei/js/jssorSlider?action=raw&ctype=text/javascript"></script>
+
<script type="text/javascript" src="https://2015.igem.org/Template:TAS_Taipei/js/jssorSlider?action=raw&amp;ctype=text/javascript"></script>
  
 
<br>
 
<br>
Line 215: Line 271:
 
var options = {
 
var options = {
 
$AutoPlay: true,
 
$AutoPlay: true,
−
                     $AutoPlayInterval: 10000,
+
                     $AutoPlayInterval: 6000,
 
$SlideDuration: 500,   
 
$SlideDuration: 500,   
 
                 $DragOrientation: 3,                                //[Optional] Orientation to drag slide, 0 no drag, 1 horizental, 2 vertical, 3 either, default value is 1 (Note that the $DragOrientation should be the same as $PlayOrientation when $DisplayPieces is greater than 1, or parking position is not 0)
 
                 $DragOrientation: 3,                                //[Optional] Orientation to drag slide, 0 no drag, 1 horizental, 2 vertical, 3 either, default value is 1 (Note that the $DragOrientation should be the same as $PlayOrientation when $DisplayPieces is greater than 1, or parking position is not 0)
Line 257: Line 313:
 
         <div u="slides" style="cursor: move; position: absolute; left: 0px; top: 0px; width: 1080px; height: 600px;
 
         <div u="slides" style="cursor: move; position: absolute; left: 0px; top: 0px; width: 1080px; height: 600px;
 
             overflow: hidden;">
 
             overflow: hidden;">
−
            <div><div u="image" style = "lightgrey;text-align:center"><image style = "width:1080px" src="https://static.igem.org/mediawiki/2015/5/5e/GroupPic.jpeg"/></div>
 
−
                <div u="caption" t="T" style="box-shadow: 0 0 10px 0;background-color:purple;padding:10px;position: absolute; top: 30px; left: 120px;border-style:solid;border-color:gold;border-width:10px">
 
−
                    <p style = "color:white;font-size:30px;">
 
−
                    <b>GRAND PRIZE WINNER
 
−
                    </p>
 
−
                    <p style = "color:white;font-size:30px;">
 
−
                    BEST WIKI</b>
 
−
                    </p>
 
−
                    <p style = "color:white;font-size: 20px; margin:0">
 
−
                    Nominated: Best Education and Public Engagement, Poster, Presentation, New Composite Part
 
−
                    </p>
 
−
                </div>
 
−
                </div>
 
 
             <div><img u="image" src="https://static.igem.org/mediawiki/2015/0/00/Tas_frontpage_1.png"/></div>
 
             <div><img u="image" src="https://static.igem.org/mediawiki/2015/0/00/Tas_frontpage_1.png"/></div>
 
             <div><div u="image" style = "lightgrey;text-align:center"><image src="https://static.igem.org/mediawiki/2015/d/df/Bg_fig6.gif"/></div>
 
             <div><div u="image" style = "lightgrey;text-align:center"><image src="https://static.igem.org/mediawiki/2015/d/df/Bg_fig6.gif"/></div>
 
                 <div u="caption" t="L" style="box-shadow: 0 0 10px 0;background-color:orange;padding:20px;position: absolute; top: 30px; left: 30px;">
 
                 <div u="caption" t="L" style="box-shadow: 0 0 10px 0;background-color:orange;padding:20px;position: absolute; top: 30px; left: 30px;">
 
                     <p style = "color:white;font-size:32px;">
 
                     <p style = "color:white;font-size:32px;">
−
                     INHIBITING GRANZYME B
+
                     INCREASING GSR/25HC
 
                     </p>
 
                     </p>
 
                     <p style = "color:white;font-size: 20px">
 
                     <p style = "color:white;font-size: 20px">
−
                     Our device produces a GzmB inhibitor
+
                     Our device produces GSR and 25HC
 
                     </p>
 
                     </p>
 
                 </div>
 
                 </div>
Line 292: Line 335:
 
             </div>
 
             </div>
 
             <div><div u="image" style = "text-align:center"><image src="https://static.igem.org/mediawiki/2015/6/6c/Tas_frontpage_2.jpeg"/></div>
 
             <div><div u="image" style = "text-align:center"><image src="https://static.igem.org/mediawiki/2015/6/6c/Tas_frontpage_2.jpeg"/></div>
−
             <div u="caption" t="L" style="box-shadow: 0 0 10px 0;background-color:orange;padding:10px;position: absolute; top: 330px; left: 20px;">
+
             <div u="caption" t="L" style="box-shadow: 0 0 10px 0;background-color:orange;padding:10px;position: absolute; top: 320px; left: 20px;">
 
                     <p style = "width:350px; color:white;font-size:22px;">
 
                     <p style = "width:350px; color:white;font-size:22px;">
−
                     "I appreciate the interdisciplinary aspect of iGEM. Aside from its strong focus on science which I enjoyed, iGEM also incorporates human, design, and technological elements."
+
                     "iGEM is special. It creates a special place for me to practice my computational biology skills."
 
                     </p>
 
                     </p>
 
                     <p style = "color:white;font-size: 16px">
 
                     <p style = "color:white;font-size: 16px">
−
                     - Joseph Chuang
+
                     - Avery Wang
 
                     </p>
 
                     </p>
 
                 </div>
 
                 </div>
Line 345: Line 388:
 
<div class = "card" id = "bodycontainer" style = "overflow:hidden;font-family:Lato; text-align:left;">
 
<div class = "card" id = "bodycontainer" style = "overflow:hidden;font-family:Lato; text-align:left;">
 
<div class = "col-sm-12">
 
<div class = "col-sm-12">
−
<h1 style = "padding:10px;background-color: rgb(33,150,243);color:white">
+
<h1 style = "padding:10px;background-color: rgb(33,50,250);color:white">
 
ABSTRACT
 
ABSTRACT
 
</h1>
 
</h1>
Line 351: Line 394:
 
             <div class = "col-sm-1"></div>
 
             <div class = "col-sm-1"></div>
 
             <div class = "col-sm-10"><br>
 
             <div class = "col-sm-10"><br>
−
<b>Elevated Granzyme B (GzmB)</b> levels are associated with many chronic inflammatory conditions including vascular, autoimmune and skin diseases. As a serine protease that induces apoptosis in tumor cells, GzmB is an essential part of the immune system. However, high levels of GzmB also result in the <b>random cleavage of extracellular matrix (ECM) proteins</b>, which leads to <b>damaged tissue structure and elasticity</b>. Our project aim is to <b>prevent tissue damage from chronic inflammation</b> by <b>limiting GzmB activity in the ECM</b> without affecting its intracellular functions. We mutated an extracellular protein (ACT) to allow it to bind and inhibit GzmB specifically in the ECM. To deliver this GzmB inhibitor into inflamed sites without bacteria entering the body, we envision two different prototypes: a bandage with a semi-permeable membrane to target localized sites, and a cream containing only the desired protein for general topical use.
+
               
 +
                <b>Cataracts</b> is the leading cause of blindness in the world. Oxidation of the lens crystallin protein, which leads to its misfolding and aggregation, is the main cause of cataracts. Among the 20 million people affected annually, over 50% are caused by old age. Currently, eye surgery is the only effective method for treatment. This method, however, is expensive and invasive. Therefore, our goal is to prevent and treat age related cataracts non-invasively by producing an eyedrop that includes <b>GSR</b> for prevention and <b>CH25H</b> for treatment. GSR catalyzes the conversion from GSSG to GSH in order to prevent crystallin oxidation. CH25H converts cholesterol into 25-hydroxycholesterol, which is a molecule that can reverse aggregation and restore solubility of the crystallin protein. To deliver proteins into the lens more efficiently, we engineered an <b>eyedrop containing biodegradable Chitosan nanoparticles</b> that encapsulate the proteins.
 
</div>
 
</div>
 
             <br>
 
             <br>
Line 367: Line 411:
  
 
<canvas id="canvas-container" style = "z-index:-1"></canvas>
 
<canvas id="canvas-container" style = "z-index:-1"></canvas>
−
<script type="text/javascript" src='https://2015.igem.org/Template:TAS_Taipei/js/field?action=raw&ctype=text/javascript'></script>
+
<script type="text/javascript" src='https://2015.igem.org/Template:TAS_Taipei/js/field?action=raw&amp;ctype=text/javascript'></script>
 
</body>
 
</body>
  
−
<!--?action=raw&ctype=text/javascript-->
+
<!--?action=raw&amp;ctype=text/javascript-->
  
 +
 +
</p>
 +
<!--
 +
NewPP limit report
 +
CPU time usage: 0.004 seconds
 +
Real time usage: 0.004 seconds
 +
Preprocessor visited node count: 4/1000000
 +
Preprocessor generated node count: 24/1000000
 +
Post‐expand include size: 0/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Highest expansion depth: 2/40
 +
Expensive parser function count: 0/100
 +
-->
 +
 +
<!-- Saved in parser cache with key 2015_igem_org:pcache:idhash:1828-0!*!*!*!*!*!* and timestamp 20151029153229 and revision id 399114
 +
-->
 +
</div> <div class="visualClear"></div>
 +
    </div>
 +
</div>
 +
    </div>
 +
</body>
 
</html>
 
</html>

Revision as of 22:18, 10 July 2016

<!DOCTYPE html> Team:TAS Taipei - 2016.igem.org

Loading menubar.....

Team:TAS Taipei

TAS Taipei iGEM Wiki




CATARACTS

INCREASING GSR/25HC

Our device produces GSR and 25HC

PROTOTYPE

We designed and built a bandage with a semi-permeable membrane

"iGEM is special. It creates a special place for me to practice my computational biology skills."

- Avery Wang

Image Slider


ABSTRACT


Cataracts is the leading cause of blindness in the world. Oxidation of the lens crystallin protein, which leads to its misfolding and aggregation, is the main cause of cataracts. Among the 20 million people affected annually, over 50% are caused by old age. Currently, eye surgery is the only effective method for treatment. This method, however, is expensive and invasive. Therefore, our goal is to prevent and treat age related cataracts non-invasively by producing an eyedrop that includes GSR for prevention and CH25H for treatment. GSR catalyzes the conversion from GSSG to GSH in order to prevent crystallin oxidation. CH25H converts cholesterol into 25-hydroxycholesterol, which is a molecule that can reverse aggregation and restore solubility of the crystallin protein. To deliver proteins into the lens more efficiently, we engineered an eyedrop containing biodegradable Chitosan nanoparticles that encapsulate the proteins.