Difference between revisions of "Team:Toronto/Experiment-Making Electrocompetent cells"
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Revision as of 06:20, 19 October 2016
Making Electrocompetent cells
Introduction
Get started by giving your protocol a name and editing this introduction.
Materials
- Reagents
- Desired cell alequot- 25mL
- LB
- Equipment
- Incubator
Procedure
- Preparing Electrocompetent cells
- Pour 25mL of desired cell into a beaker and add 475mL of LB
- incubate at 37oC for 2 hours
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