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Latest revision as of 06:57, 19 October 2016

console.js:26

Monday June 20, 2016

Monday, 6/20
Members Present: Alex, Cathy, Kat, Hamed, Marc, Celene, Dk, Andrea
LAB:
Morning:
- Ran gel electrophoresis protocol using RFP and various amounts of DNA ladder found in the fridge (3ul, 6ul, 10ul) [picture to follow]
gel 1.jpg
thumbnail
GEL: LANE 1: 3uL of 3kb DNA ladder. LANE 2: 6 uL of DNA ladder. LANE 3: 9uL of DNA ladder. LANE 4: Miniprepped RFP. Note nonspecific banding. LANE 5: Loading Dye (not visible)
Concluded that 6uL of DNA ladder should be sufficient.
Afternoon:
Meeting with Dr. Pardee & Christian Euler
Administrative:
TO DO:
For the next day:
LAB TEAM:
LAB MANAGERS:
- Please buy a USB! (something cheap will do)
- Check the prices for the S30/S12 reagents (and if we have them in our inventory or the Biozone inventory):
ATP; GTP, UTP, CTP;
L-5-formyl-5, 6, 7, 8-tetrahydrofolic acid (folinic acid);
potassium glutamate;
ammonium glutamate;
magnesium glutamate;
20 amino acids (is there a mix?);
L-[14C(U)]-leucine;
nicotinamide adenine dinucleotide (NAD);
coenzyme-A (CoA);
spermidine;
putrescine;
sodium oxalate;
phosphoenolpyruvate (PEP);
DTT
NaH2PO4
Na2HPO4
A
B
C
D
E
1
MONDAYTUESDAYWEDNESDAYTHURSDAYFRIDAY
2
Gel electrophoresis on RFPRubidium chloride competent cells
3
Restriction enzyme digest (using the iGEM protocol)
4
Gel electrophoresis
Table1
Gmail correspondence:
Meetings/Notes:
Notes from General Meeting:
0620 Notes General Meeting.docx
References: