Add 4 volumes of PureLink ® Binding Buffer (B2) with isopropanol to 1 volume of the PCR product (50–100 μL). Mix well.

Remove a PureLink ® Spin Column in a Collection Tube from the package.

Add the sample with the appropriate Binding Buffer (from step 1 of this procedure) to the PureLink ® Spin Column.

Centrifuge the column at room temperature at 13,000 × g for 1 minute.

Discard the flow through and place the spin column into the collection tube.

Add 650 μL of Wash Buffer with ethanol to the column.

Centrifuge th e column at room temperature at 13,000 × g for 1 minute. Discard the flow through from the collection tube and place the column into the tube.

Centrifuge the column at maximum speed (15, 000 x g)at room temperature for 2–3 minutes to remove any residual Wash Buffer. Discard the collection tube.

Place the spin column in a clean 1.7-mL PureLink ® Elution Tube supplied with the kit.

Add 50 μL of Elution Buffer (10 mM Tris-HCl, pH 8.5) or sterile, distilled water (pH >7.0) to the center of the column.

Incubate the column at room temperature for 1 minute. Centrifuge the column at maximum speed for 2 minutes.

The elution tube contains the purified PCR product. Remove and discard the column. Store the purified PCR product at –20°C or use the PCR product for the desired downstream application.