Difference between revisions of "Team:Lanzhou"

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<title>Main</title>
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  <link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
  
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<h2> Welcome to iGEM 2016! </h2>
 
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
 
  
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<h2> Brief Introduction </h2>
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<p>Heavy metal contamination has become a serious problem in fishery in recent years. Now with Lanzhou's GM bacteria, the heavy metal ions can be captured in the fish gut. This can be a new method to solve the problem./</p>
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
 
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</ul>
 
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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  src: url('http://of5b4n94d.bkt.clouddn.com/fonts/4/ANGEL.otf');
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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</div>
 
  
  
  
  
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<h5> Editing your wiki </h5>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
  
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<h5>Tips</h5>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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</ul>
 
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</div>
 
  
  
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<div class="column half_size" >
 
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<h5>Inspiration</h5>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<ul>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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</ul>
 
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</div>
 
  
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<div class="column half_size" >
 
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<h5> Uploading pictures and files </h5>
 
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
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                <li class="active"><a href="badges">Main</a></li>
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                <li><a href="badges">Attributions</a></li>
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                <li><a href="badges">Safety</a></li>
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                <li><a href="badges">Notes</a></li>
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UPLOAD FILES
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          Main
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                  Project Description
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              <p style="text-align: justify;">
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                <i>Mercury is a poisonous heavy metal that poses a threat to aquatic life and humans. Recently, increasing attention has been paid to mercury remediation and its detection in the environment by biological technology. In our project, a series of mercury-specific binding peptide termed metal catcher (MC), which has a high affinity and selectivity toward mercury, was examined. The subsequent IC and GFP were fused and displayed on Escherichia coli cell surface by using an N-terminal region ice nucleation protein anchor. </i>
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        <div class="col s12 l12">
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          <p style="text-align: justify;">
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            <i>Whole-cell sorbents of construction facilitated selective adsorption of mercury ions from a solution containing different heavy metal ions and detection mercury and cooper. The MC aided rapid detection of copper, mercury, and cadmium in 2 min with a low detection limit (1 uM).Additionally, mercury levels were reduced by approximately 36% by using the MC. The transformant strains were then fed to Cyprinus carpio and colonized in the microbiota. C. carpio with transformants showed significantly lower accumulation of heavy metals compared to the control group. The control group was E. coli BL21 containing a pET23b plasmid without any exogenous gene. Moreover, the metal remediation ability of the microbiota with transformants was improved by Illumina MiSeq 16S rRNA amplicon sequencing. The surface-engineered E. coli effectively protected fish from the toxicity of mercury ions at high concentrations in an aquatic environment. Thus, gut remediation is an ideal approach to control heavy metal contamination in fish.</i>
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                  Please Visit Us
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                </h3>
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                <h5 class="right-align">
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                    Oct. 29th 1:30AM Room 304
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                </h5>
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          <h5 class="white-text">LZU-China 2016</h5>
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          <p class="grey-text text-lighten-4">NO.222, TianShui South Road, Lanzhou, China, 730000</p>
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          <h6 class="white-text">Copyright 2015 Lanzhou University</h6>
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          <h5 class="white-text">Acknowledgments</h5>
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            <li><a class="white-text" href="http://en.lzu.edu.cn/">Educational Administration Office, LZU</a></li>
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            <li><a class="white-text" href="http://chc.lzu.edu.cn/en/">Cuiying Honors College, LZU</a></li>
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            <li><a class="white-text" href="http://lifesc.lzu.edu.cn/">School of Life Sciences,LZU</a></li>
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Revision as of 07:48, 19 October 2016

Main

Main

Project Description

Mercury is a poisonous heavy metal that poses a threat to aquatic life and humans. Recently, increasing attention has been paid to mercury remediation and its detection in the environment by biological technology. In our project, a series of mercury-specific binding peptide termed metal catcher (MC), which has a high affinity and selectivity toward mercury, was examined. The subsequent IC and GFP were fused and displayed on Escherichia coli cell surface by using an N-terminal region ice nucleation protein anchor.

Whole-cell sorbents of construction facilitated selective adsorption of mercury ions from a solution containing different heavy metal ions and detection mercury and cooper. The MC aided rapid detection of copper, mercury, and cadmium in 2 min with a low detection limit (1 uM).Additionally, mercury levels were reduced by approximately 36% by using the MC. The transformant strains were then fed to Cyprinus carpio and colonized in the microbiota. C. carpio with transformants showed significantly lower accumulation of heavy metals compared to the control group. The control group was E. coli BL21 containing a pET23b plasmid without any exogenous gene. Moreover, the metal remediation ability of the microbiota with transformants was improved by Illumina MiSeq 16S rRNA amplicon sequencing. The surface-engineered E. coli effectively protected fish from the toxicity of mercury ions at high concentrations in an aquatic environment. Thus, gut remediation is an ideal approach to control heavy metal contamination in fish.

Please Visit Us

Oct. 29th 1:30AM Room 304