Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W11"
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<th> Trial 1 (ul) </th> | <th> Trial 1 (ul) </th> | ||
<th> Trial 2 (ul) </th> | <th> Trial 2 (ul) </th> | ||
| + | <th> </th> | ||
</tr> | </tr> | ||
<td> Phire </td> | <td> Phire </td> | ||
Revision as of 07:53, 19 October 2016
Week 11: 9/5 - 9/11
9/5
Digest of CR-1 Plasmids
| Content | Experimental ul | Control ul |
|---|---|---|
| H2O | 15.5 | 16.5 |
| Cutsmart | 2.5 | 2.5 |
| XhoI | 0.5 | X |
| EcoRI-HF | 0.5 | X |
| DNA | 6 | 6 |
PCR of Toggle 2
PCR
| Contents | Trial 1 (ul) | Trial 2 (ul) | Q5 MasterMix | 12.5 | 12.5 |
|---|---|---|
| H2O | 6.5 | 5.5 |
| 10 uM F Primer | 0.5 | 1.0 |
| 10 uM R Primer | 0.5 | 1.0 |
| 100pg/ul Construct | 5 | 5 |
PCR Settings: 30 cycles
| Phase | Temperature (°C) | Time (sec) |
|---|---|---|
| Initial Denaturation | 98 | 30 |
| Denaturation | 98 | 10 |
| Annealing | 68 | 20 |
| Extension | 72 | 60 |
| Final Extension | 72 | 120 |
Ran Digests
Digest of J6100
| Content | Experimental ul |
|---|---|
| H2O | 7.5 |
| Cutsmart | 1.0 |
| XbaI | 0.5 |
| DNA | 1.0 |
Digest of CR-1
| Content | Experimental ul | Control ul |
|---|---|---|
| H2O | 5.0 | 6.0 |
| Cutsmart | 1.0 | 1.0 |
| EcoRI-HF | 0.5 | X |
| XbaI | 0.5 | X |
| DNA | 3 | 3 |
Digest of Dean Vector #3
| Content | Experimental ul | Control ul |
|---|---|---|
| H2O | 2.0 | 3.0 |
| Cutsmart | 1.0 | 1.0 |
| EcoRI-HF | 0.5 | X |
| XhoI | 0.5 | X |
| Vector | 6 | 6 |
PCR
| Contents | Trial 1 (ul) | Trial 2 (ul) | Phire | 25.0 | 25.0 | 25.0 |
|---|---|---|---|
| H2O | 21.5 | 19.0 | 14.0 |
| 10 uM F Primer | 1.25 | 1.50 | 5.0 |
| 10 uM R Primer | 1.25 | 1.50 | 5.0 |
| 100pg/ul Construct | 1 | 1 | 1 |
PCR Settings: 30 cycles
| Phase | Temperature (°C) | Time (sec) |
|---|---|---|
| Initial Denaturation | 98 | 30 |
| Denaturation | 98 | 5 |
| Annealing | 66 | 10 |
| Extension | 72 | 20 |
| Final Extension | 72 | 60 |
