Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-mRFP1"
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<li><p style="font-size: 18px;">mRFP1 PCR amplification</p></li> | <li><p style="font-size: 18px;">mRFP1 PCR amplification</p></li> | ||
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p> | ||
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Latest revision as of 10:24, 19 October 2016
mRFP1
mRFP1
7/9
BBa_E1010 T-streaked onto agar plates
7/10
BBa_E1010 extracted the checked with restriction digestion (EocRI and PstI, 747bp and 2029bp)
7/16
BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)
7/17
BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)
7/18
BBa_E1010 checked with plasmid PCR with newly design primers
7/19
BBa_E1010 checked with plasmid PCR with the addition of Mg2+
7/22
BBa_E1010 checked with Taq polymerase plasmid PCR (successful)
8/13
8/16
mRFP1 digestion
8/19
mRFP1 PCR amplification (failed)
8/21
8/25
mRFP1 checked with PCR due to multiple failed double ligation
8/26
9/5
mRFP1 amplified with KOD polymerase PCR
9/9
mRFP1 amplified with KOD polymerase PCR
