Difference between revisions of "Team:Bulgaria"

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{{Bulgaria}}
 
 
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<h2> Project Description</h2>
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<p class = "description">With the dawn of synthetic biology and the ever increasing utilization of microfluidics in experimental practice a new understanding of single-cell biology has emerged. We now realize a lot more of the inner mechanisms that regulate individual cells.
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<p class = "description">...Our team aims to make the detection of carbon, phosphate and nitrogen starvation in cultured cells easier and faster. In order to accomplish this we will insert different fluorescent protein genes in a bacterial plasmid. Those will be expressed together with Escherichia coli’s starvation genes and this way we will observe light in different parts of the spectrum according to the needs of the cell culture. After stimulating the transformed cells the fluorescence will be detected through signal monitoring system. The data will be used to visualize the level of expression i.e. the phase of starvation.
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<p class = "description">We want to make a cell culture that is cheap, easy to cultivate and that shows us exactly what it lacks in its environment. If we are successful this approach could be used in many different fields including pharmacy, bio-engineering, improving the efficiency of bioreactors in general and many more.
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We have our strains and successfully made our first electroporation.
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                <a href="index.html">home</a>
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 +
            <li class="menu-item drop-link"> project
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                    <li class="sub-item"><a href="overview.html">overview</a></li>
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                    <li class="sub-item"><a href="results.html">results</a></li>
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                    <li class="sub-item"><a href="futureWork.html">future work</a></li>
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                    <li class="sub-item"><a href="organisms.html">organisms</a></li>
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                    <li class="sub-item"><a href="safety.html">safety</a></li>
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            <li class="menu-item">lab</li>
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 +
            <li class="menu-item drop-link">achievements
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            <li class="menu-item drop-link">human practices
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                    <li class="sub-item"><a href="integratedHP.html">integrated human practices</a></li>
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                    <li class="sub-item"><a href="collaboration.html">collaboration</a></li>
 +
                    <li class="sub-item"><a href="entrepreneurship.html">enterpreneurship</a></li>
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 +
            </li>
 +
 
 +
            <li class="menu-item drop-link">team
 +
                <ul class="team-submenu dropdown-content">
 +
                    <li class="sub-item"><a href="members.html">members</a></li>
 +
                    <li class="sub-item"><a href="ourStory.html">our story</a></li>
 +
                    <li class="sub-item"><a href="attributions.html">attributions</a></li>
 +
                    <li class="sub-item"><a href="university.html">university</a></li>
 +
                    <li class="sub-item"><a href="bulgaria.html">Bulgaria</a></li>
 +
                </ul>
 +
            </li>
 +
        </ul>
 +
    </nav>
 +
    <header>
 +
        <div class="header-image">
 +
            <img class="igem-logo" src="https://static.igem.org/mediawiki/2016/9/92/Team-Bulgaria-logo.png" alt="iGem">
 +
        </div>
 +
    </header>
 +
    <main>
 +
        <div class="description">
 +
            <p class="description-project">
 +
                With the dawn of synthetic biology and the ever increasing utilization of microfluidics
 +
                in experimental practice a new understanding of single-cell biology has emerged. We now
 +
                realize a lot more of the inner mechanisms that regulate individual cells.
 +
 
 +
                Our team aims to make the detection of carbon, phosphate and nitrogen starvation in
 +
                cultured cells easier and faster. In order to accomplish this we will insert different
 +
                fluorescent protein genes in a bacterial plasmid. Those will be expressed together
 +
                with Escherichia coli’s starvation genes and this way we will observe light
 +
                in different parts of the spectrum according to the needs of the cell culture.
 +
                After stimulating the transformed cells the fluorescence will be detected through
 +
                signal monitoring system. The data will be used to visualize the level of expression
 +
                i.e. the phase of starvation.
 +
 
 +
                We want to make a cell culture that is cheap, easy to cultivate and that shows us
 +
                exactly what it lacks in its environment. If we are successful this approach could
 +
                be used in many different fields including pharmacy, bio-engineering, improving the
 +
                efficiency of bioreactors in general and many more. We have our strains and successfully
 +
                made our first electroporation.
 +
            </p>
 +
        </div>
 +
 
 +
 
 +
        <div class="introduction">
 +
            <ul class="content">
 +
                <li class="single-part">
 +
                    <article class="box">
 +
                        <img src="https://static.igem.org/mediawiki/2016/6/6b/Team-Bulgaria-icon1.png" alt="Nothing can stop us" class="icon">
 +
                        <h3 class="box-title">nothing can stop us</h3>
 +
                        <p class="icon-description">
 +
                            The participation in the competition iGEM is making us
 +
                            the first Bulgarian team, and that's why this cause is
 +
                            so important for us. Our responsibility is even greater
 +
                            and our desire to succeed and achieving it - more secure.
 +
                        </p>
 +
                    </article>
 +
                </li>
 +
 
 +
                <li class="single-part">
 +
                    <article class="box">
 +
                        <img src="https://static.igem.org/mediawiki/2016/c/c8/Team-Bulgaria-icon2.png" alt="Puzzel" class="icon">
 +
                        <h3 class="box-title">we solve problems together</h3>
 +
                        <br>
 +
                        <p class="icon-description">
 +
                            We solve all the difficulties together because we are one real team.
 +
                        </p>
 +
                        <br>
 +
                    </article>
 +
                </li>
 +
 
 +
                <li class="single-part">
 +
                    <article class="box">
 +
                        <img src="https://static.igem.org/mediawiki/2016/5/5e/Team-Bulgaria-icon3.png" alt="Bulb" class="icon">
 +
                        <h3 class="box-title">we work with passion</h3>
 +
                        <p class="icon-description">
 +
                            Our love in biology is the unifying force that brought
 +
                            together people with different professions, personalities,
 +
                            interests. The passion for science is the reason we all fight
 +
                            together for the realisation of the ultimate goal.
 +
                        </p>
 +
                    </article>
 +
                </li>
 +
            </ul>
 +
        </div>
 +
 
 +
        <div class="partners">
 +
            <h2 class="partners-title">our partners</h2>
 +
 
 +
            <ul class="partner-list">
 +
                <li class="partner">
 +
                    <img src="https://static.igem.org/mediawiki/2016/6/64/Nadezhda-logo.png" alt="nadezhda womens hospital" class="partner-logo">
 +
                    <h4 class="partner-title">nadezhda women's health hospital</h4>
 +
                </li>
 +
 
 +
                <li class="partner">
 +
                    <img src="https://static.igem.org/mediawiki/2016/9/9e/Su-logo3.png" alt="SU Council" class="partner-logo">
 +
                    <h4 class="partner-title">sofia university student council</h4>
 +
                </li>
 +
 
 +
                <li class="partner">
 +
                    <img src="https://static.igem.org/mediawiki/2016/8/8b/Biovet-logo.png" alt="Biovet" class="partner-logo">
 +
                    <h4 class="partner-title">biovet</h4>
 +
                </li>
 +
 
 +
                <li class="partner">
 +
                    <img src="https://static.igem.org/mediawiki/2016/6/65/Tech-park-logo.png" alt="Sofia Tech Park" class="partner-logo">
 +
                    <h4 class="partner-title">sofia techpark</h4>
 +
                </li>
 +
 
 +
                <li class="partner">
 +
                    <img src="https://static.igem.org/mediawiki/2016/2/23/Fox-laser-logo.png" alt="Fox Laser" class="partner-logo">
 +
                    <h4 class="partner-title">fox laser</h4>
 +
                </li>
 +
 
 +
                <li class="partner">
 +
                    <img src="https://static.igem.org/mediawiki/2016/9/97/Tu-logo.png" alt="TU Sofia" class="partner-logo">
 +
                    <h4 class="partner-title">technical university sofia</h4>
 +
                </li>
 +
 
 +
                <li class="partner">
 +
                    <img src="https://2016.igem.org/File:Su-logo2.png" alt="SU" class="partner-logo">
 +
                    <h4 class="partner-title">sofia university</h4>
 +
                </li>
 +
 
 +
                <li class="partner">
 +
                    <img src="https://static.igem.org/mediawiki/2016/3/3d/Su-logo.png" alt="Student Council" class="partner-logo">
 +
                    <h4 class="partner-title">student council</h4>
 +
                </li>
 +
            </ul>
 +
        </div>
 +
    </main>
 +
 
 +
</div>
 +
</body>
 
</html>
 
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Revision as of 10:42, 19 October 2016

With the dawn of synthetic biology and the ever increasing utilization of microfluidics in experimental practice a new understanding of single-cell biology has emerged. We now realize a lot more of the inner mechanisms that regulate individual cells. Our team aims to make the detection of carbon, phosphate and nitrogen starvation in cultured cells easier and faster. In order to accomplish this we will insert different fluorescent protein genes in a bacterial plasmid. Those will be expressed together with Escherichia coli’s starvation genes and this way we will observe light in different parts of the spectrum according to the needs of the cell culture. After stimulating the transformed cells the fluorescence will be detected through signal monitoring system. The data will be used to visualize the level of expression i.e. the phase of starvation. We want to make a cell culture that is cheap, easy to cultivate and that shows us exactly what it lacks in its environment. If we are successful this approach could be used in many different fields including pharmacy, bio-engineering, improving the efficiency of bioreactors in general and many more. We have our strains and successfully made our first electroporation.

  • Nothing can stop us

    nothing can stop us

    The participation in the competition iGEM is making us the first Bulgarian team, and that's why this cause is so important for us. Our responsibility is even greater and our desire to succeed and achieving it - more secure.

  • Puzzel

    we solve problems together


    We solve all the difficulties together because we are one real team.


  • Bulb

    we work with passion

    Our love in biology is the unifying force that brought together people with different professions, personalities, interests. The passion for science is the reason we all fight together for the realisation of the ultimate goal.

our partners

  • nadezhda women's health hospital

  • sofia university student council

  • biovet

  • sofia techpark

  • fox laser

  • technical university sofia

  • sofia university

  • student council