Difference between revisions of "Team:ASIJ Tokyo/Parts"

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<br>
 
<br>
 
Andersen Promoters
 
Andersen Promoters
−
<li> Strength: weak (0.33) BBa_J23110
+
<li>Relative Strength: weak (0.33) BBa_J23110
 
         <ul>
 
         <ul>
−
             <li>Wanted a weak promoter to measure its PET plastic degradation up against the other promoters</li>
+
             <li>Selected as a relatively weak promoter against which to compare the expression of the PETase construct</li>
 
           </ul>
 
           </ul>
 
</li>
 
</li>
−
<li>Strength: moderate (0.58) BBa_J23111
+
<li>Relative Strength: moderate (0.58) BBa_J23111
 
           <ul>
 
           <ul>
−
             <li>Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters</li>
+
             <li>Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct </li>
 
           </ul>
 
           </ul>
 
  </li>
 
  </li>
−
<li>Strength: strong (1) BBa_J23100
+
<li>Relative Strength: strong (1) BBa_J23100
 
           <ul>
 
           <ul>
−
             <li>Wanted a strong promoter to measure its PET plastic degradation up against the other promoters</li>
+
             <li>Selected as a relatively strong promoter against which to compare the expression of the PETase construct</li>
 
           </ul>
 
           </ul>
 
</li>
 
</li>
−
<li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest</li>
+
<li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli</li>
−
<li>C-Myc tag: Make our PETase construct easier to detect for Western     Blot to assay expression and secretion</li>
+
<li>C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression </li>
−
<li>PETase sequence: Needed the enzyme to degrade PET plastic</li>
+
<li>PETase sequence: gene that coded for the PET hydrolase</li>
−
<li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly</li>
+
<li>Plasmid backbone: the pSB1C3 backbone is recommended </li>
−
<li>High efficiency e. Coli cells: Were the host for our optimized PETase</li>
+
<li>High efficiency e. Coli cells: hosted the PETase constructs</li>
  
  

Revision as of 11:22, 19 October 2016

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Parts


Andersen Promoters
  • Relative Strength: weak (0.33) BBa_J23110
    • Selected as a relatively weak promoter against which to compare the expression of the PETase construct
  • Relative Strength: moderate (0.58) BBa_J23111
    • Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct
  • Relative Strength: strong (1) BBa_J23100
    • Selected as a relatively strong promoter against which to compare the expression of the PETase construct
  • N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli
  • C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression
  • PETase sequence: gene that coded for the PET hydrolase
  • Plasmid backbone: the pSB1C3 backbone is recommended
  • High efficiency e. Coli cells: hosted the PETase constructs