Difference between revisions of "Team:Vilnius-Lithuania/Methods"

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<div class="column full_size" >
 
<div class="column full_size" >
  
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<h1 class="Raleway" style="text-align:center;">Results</h1>
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<h1 class="Raleway" style="text-align:center;">Methods</h1>
 
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<span class="line" style="margin:0 auto;"> </span>
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<!-- MethodBox -->
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<a href='#' class='center'><img src="https://static.igem.org/mediawiki/2016/5/5e/T--Vilnius-Lithuania--projectiliustracija.png"></a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Description' class='deg170'>Description</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Design' class='deg150'>Design</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Dry_Lab' class='deg130'>Dry_Lab</a>
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            <h4 class="panel-title">1. Plasmid DNA extraction</h4>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Experiments' class='deg110'>Experiments</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Results' class='deg90'>Results</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Safety' class='deg10'>Safety</a>
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                <div class="col-md-4">
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                    <strong>Materials:</strong><br />
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                    4 mL of LB medium<br />
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                    Antibiotic<br />
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                    Transformed bacteria<br />
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                </div>
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                <div class="col-md-8">
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                    <strong>Process</strong>:<br />
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                    Transformed bacteria, which contain a plasmid with a target DNA, is placed into laboratory tube with 4 ml of liquid LB medium and specific antibiotic. Bacteria are then placed into the shaker during the night at 37ºC. For the plasmid DNA extraction we used GeneJet Plasmid Miniprep Kit, according to manufacturer's <a class="clink" href="https://static.igem.org/mediawiki/2015/b/b1/Vilnius15_PlasmidMiniprep.pdf">instructions.</a>
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Revision as of 11:31, 19 October 2016

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Methods

Materials:
4 mL of LB medium
Antibiotic
Transformed bacteria
Process:
Transformed bacteria, which contain a plasmid with a target DNA, is placed into laboratory tube with 4 ml of liquid LB medium and specific antibiotic. Bacteria are then placed into the shaker during the night at 37ºC. For the plasmid DNA extraction we used GeneJet Plasmid Miniprep Kit, according to manufacturer's instructions.