Difference between revisions of "Team:UPMC-Paris/Material"

Kits

1. .Monarch Plasmid Miniprep Kit from NEB Ref: T1010S
2. .Monarch PCR & DNA Cleanup Kit (5ug) Ref: T1030S
3. .Phusion High-Fidelity PCR Kit from NEB Ref: E0553S
4. .QiAquick Gel Extraction Kit from Qiagen Ref: 28704
5. .QiAquick PCR Purification Kit from Qiagen Ref: 28104

Bacterial Strains

You can find our bacteria here!

Media and chemicals

.LB Broth-Miller from Difco, Ref: 244620
.Bacto Yeast Extract from Difco, Ref: 212750
.Quick-Load 1 kb DNA Ladder from NEB, Ref: N0468S
.Formic Acid Free Acid from Sigma-Aldrich, Ref: F0507-100ML-D
.Manganese(II) Sulfate Monohydrate from Sigma-Aldrich, Ref: M7634-100G-D
.Zinc Sulfate Hepthydrate from Sigma-Aldrich, Ref: Z4750-100G-D
.Ferric Chloride Hexahydrate from Sigma-Aldrich, Ref: F2877-100G-D
.Thiamine Hydrochloride from Sigma-Aldrich, Ref: T4625-5G
.Chelex 100 Sodium Form (Chelating Resin) from Sigma-Aldrich, Ref: C7901-25G
.
.Nicotinic Acid 99% from Grosseron, Ref: A12683.30
.Phusion High-Fidelity PCR Master Mix with HF Buffer from Thermo Fisher Scientific, Ref: F531S
.

Enzymes

.Pvu II, recombinant from NEB, Ref: R0151S
.Avr II, recombinant from NEB, Ref: R0174S
.Pac I, recombinant from NEB, Ref: R0547S
.Psc I from Thermo Fisher Scientific, Ref: ER1871
.Kpn I from Thermo FIsher Scientific, Ref: ER0521
.EcoR I
.Xba I
.Spe I
.Sac I
.Kpn I
.BamH I
.Sal I
.T4 DNA Ligase from Sigma-Aldrich, Ref: 10481220001

Antibiotics

.Cloramphenicol from Sigma-Aldrich, Ref: C0378-5G
.Kanamycine sulfate from Termo Fisher Scientific, Ref: 11815024

DNA and Plasmids

Our PCR primers were synthesized by Eurofins
Our Biobricks fragments were synthesized by IDT