Difference between revisions of "Team:ASIJ Tokyo/Parts"

Line 8,013: Line 8,013:
 
                   <h1>Parts </h1>
 
                   <h1>Parts </h1>
 
<br>
 
<br>
−
<h2>Anderson Promoters</h2>
+
<h3>Anderson Promoters</h3>
 
<li>Relative Strength: weak (0.33) BBa_J23110
 
<li>Relative Strength: weak (0.33) BBa_J23110
 
         <ul>
 
         <ul>
Line 8,030: Line 8,030:
 
</li>
 
</li>
 
<br>
 
<br>
−
<h2>Tags</h2>
+
<h3>Tags</h3>
 
<li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli</li>
 
<li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli</li>
 
<li>C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression </li>
 
<li>C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression </li>
 
<br>
 
<br>
−
<h2>Others</h2>
+
<h3>Others</h3>
 
<li>PETase sequence: gene that coded for the PET hydrolase</li>
 
<li>PETase sequence: gene that coded for the PET hydrolase</li>
 
<li>Plasmid backbone: the pSB1C3 backbone is recommended </li>
 
<li>Plasmid backbone: the pSB1C3 backbone is recommended </li>
 
<li>High efficiency e. Coli cells: hosted the PETase constructs</li>
 
<li>High efficiency e. Coli cells: hosted the PETase constructs</li>
 
<br>
 
<br>
−
<h2>Parts Submitted</h2>
+
<h3>Parts Submitted</h3>
 
<li>BBa_K1885001</li>
 
<li>BBa_K1885001</li>
 
<li>BBa_K1885002</li>
 
<li>BBa_K1885002</li>

Revision as of 14:12, 19 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Parts


Anderson Promoters

  • Relative Strength: weak (0.33) BBa_J23110
    • Selected as a relatively weak promoter against which to compare the expression of the PETase construct
  • Relative Strength: moderate (0.58) BBa_J23111
    • Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct
  • Relative Strength: strong (1) BBa_J23100
    • Selected as a relatively strong promoter against which to compare the expression of the PETase construct

    • Tags

  • N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli
  • C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression

    • Others

  • PETase sequence: gene that coded for the PET hydrolase
  • Plasmid backbone: the pSB1C3 backbone is recommended
  • High efficiency e. Coli cells: hosted the PETase constructs

    • Parts Submitted

  • BBa_K1885001
  • BBa_K1885002
  • BBa_K1885005