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The first step relies on the affinity of siderophores to bind solubilized Platinum atoms and, thus, favor the further solubilisation of more platinum compounds. We accomplish this by inserting a plasmid containing the four enzymes (Des A, Des B, Des C, Des D) necessary to synthesise our siderophore — Desferrioxamine B, into E. coli. | The first step relies on the affinity of siderophores to bind solubilized Platinum atoms and, thus, favor the further solubilisation of more platinum compounds. We accomplish this by inserting a plasmid containing the four enzymes (Des A, Des B, Des C, Des D) necessary to synthesise our siderophore — Desferrioxamine B, into E. coli. | ||
− | At a second level, we plan to use the principle of biosorption. A modified | + | At a second level, we plan to use the principle of biosorption. A modified FliC protein complex will be cloned into E. coli and enable the flagella of the bacterium to bind platinum atoms. This specificity will be possible thanks to a peptide that will be inserted into the sequence of the FliC protein. |
The benefit of using the biosorption is to obtain nanoparticles of platinum, a highly valuable form of the metal. | The benefit of using the biosorption is to obtain nanoparticles of platinum, a highly valuable form of the metal. | ||
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