Difference between revisions of "Team:Toronto/Notebook-w16-wed"

 
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</li>
 
</li>
 
<li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li>
 
<li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li>
</li>
 
<li><a href="https://2016.igem.org/Team:Toronto/HP-Impact"><span>impact</span></a></li>
 
 
</li>
 
</li>
 
<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li>
 
<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li>

Latest revision as of 15:14, 19 October 2016

console.js:26

Wednesday, August 31

Wednesday, 8/31
Members Present:
LAB:
Morning:
Miniprepped cultures made yesterday (as well as LacZ from the day before):
A
B
C
D
1
Sample260/280260/230conc (ng/uL)
2
LacZ1.811.36106.9
3
SP1.91.5795.3
4
SG1.690.98150.3
5
P1.781.1781.7
6
11.740.9251.9
7
21.680.9263.5
Miniprep - August 31st
Nore that 1 and 2 were used to denote the O/N cultures that were in the fridge, as their labels had worn off
Made O/N cultures (5ml) of the remainder for further miniprep
Made O/N cultures from the transformants from the previous day. SG was unsuccessful, therefore it was transformed once more
Made aliquots of nf H20
Made more LB + CAM plates
Afternoon:
Administrative:
Talked to Kayla, she promised to send us the junk DNA for our LacZ omega construct
TO DO:
For the next day:
LAB TEAM:
Gel extract the constructs that were unsuccessful
Miniprep the O/N cultures.
LAB MANAGERS:
Buy more falcon tubes
FInd where our missing p200 is
Gmail correspondence:
Meetings/Notes:
References: