Difference between revisions of "Team:Aachen/Integrated Practices"

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  <p align="justify" style="padding-left: 1.0cm; padding-right: 1.0cm; font-size:16px;">We have also taken into account the needs and thoughts of the public to improve our project.
 
  <p align="justify" style="padding-left: 1.0cm; padding-right: 1.0cm; font-size:16px;">We have also taken into account the needs and thoughts of the public to improve our project.
 
<br/>
 
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[image here]
 
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<div class="single_header_title">
 
             <h1 style="padding-left: 0.8cm;">Dark Bench</h1>
 
             <h1 style="padding-left: 0.8cm;">Dark Bench</h1>
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<p align="justify" style="padding-left: 1.0cm; padding-right: 1.0cm; font-size:16px;">
 
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<span style="color:#005C04">“Or, if someone else built it for you, how much would it cost.”</span>
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<center style="color:#005C04">“Or, if someone else built it for you, how much would it cost.”</center>
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<figcaption style="font-size: 12px; position:relative;top: -20px; left: 100px; color:Grey;">Dr. David Mishler, Austin, Texas</figcaption>
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<figcaption style="font-size: 12px; position:relative;top: -20px; left: 400px; color:Grey;">Dr. David Mishler, Austin, Texas</figcaption>
 
<p align="justify" style="padding-left: 1.0cm; padding-right: 1.0cm; font-size:16px;">
 
<p align="justify" style="padding-left: 1.0cm; padding-right: 1.0cm; font-size:16px;">
 
That was Dr. Mishlers concern, when he gave us some feedback to the design of our model. At first he seemed to be interested in our Hardware part, but was skeptical if having the advantages would be worth the price. We estimated the costs to build one darkbench at about 200 $. <br/><br/>
 
That was Dr. Mishlers concern, when he gave us some feedback to the design of our model. At first he seemed to be interested in our Hardware part, but was skeptical if having the advantages would be worth the price. We estimated the costs to build one darkbench at about 200 $. <br/><br/>
−
Click <here>  for the detailed calculation.<br/><br/>
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Detailed calculation.<br/><br/>
 +
 
 +
<table class="wiki_table" style="border-collapse:separate;border:0px;border-spacing:0px;margin-top:0px;width: auto;table-layout: fixed; margin-right: 85px;margin-left: 85px;">
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<tr>
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<th style="border:1pt solid black;background-color:#7fb983;">Component</th>
 +
<th style="border:1pt solid black;background-color:#7fb983;">Specifications</th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"> Quantity</th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"> Costs<sup>*</sup> <br>[&euro;/piece]</br> </th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"> Costs<sup>*</sup> <br>[&#36;/piece]</br> </th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"> Final<sup>*</sup> <br>[&euro;]</br> </th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"> Final<sup>*</sup> <br>[&#36;]</br></th>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Opaque plexiglas 4mm</td>
 +
<td style="border:1pt solid black;">50X100 cm</td>
 +
<td style="border:1pt solid black;">5</td>
 +
<td style="border:1pt solid black;">22.45</td>
 +
<td style="border:1pt solid black;">24.63</td>
 +
<td style="border:1pt solid black;">112.25</td>
 +
<td style="border:1pt solid black;">123.15</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Transparent plexiglas 3mm</td>
 +
<td style="border:1pt solid black;">25X50 cm</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">7.5</td>
 +
<td style="border:1pt solid black;">8.23</td>
 +
<td style="border:1pt solid black;">7.5</td>
 +
<td style="border:1pt solid black;">8.23</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Screws</td>
 +
<td style="border:1pt solid black;">M3X10</td>
 +
<td style="border:1pt solid black;">155</td>
 +
<td style="border:1pt solid black;">0.05</td>
 +
<td style="border:1pt solid black;">0.05</td>
 +
<td style="border:1pt solid black;">7.75</td>
 +
<td style="border:1pt solid black;">7.75</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Screws</td>
 +
<td style="border:1pt solid black;">M3X16</td>
 +
<td style="border:1pt solid black;">10</td>
 +
<td style="border:1pt solid black;">0.08</td>
 +
<td style="border:1pt solid black;">0.09</td>
 +
<td style="border:1pt solid black;">0.8</td>
 +
<td style="border:1pt solid black;">0.9</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Screws</td>
 +
<td style="border:1pt solid black;">M3X20</td>
 +
<td style="border:1pt solid black;">25</td>
 +
<td style="border:1pt solid black;">0.09</td>
 +
<td style="border:1pt solid black;">0.10</td>
 +
<td style="border:1pt solid black;">2.25</td>
 +
<td style="border:1pt solid black;">2.5</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Washer</td>
 +
<td style="border:1pt solid black;">3.2mm</td>
 +
<td style="border:1pt solid black;">200</td>
 +
<td style="border:1pt solid black;">0.02</td>
 +
<td style="border:1pt solid black;">0.02</td>
 +
<td style="border:1pt solid black;">4</td>
 +
<td style="border:1pt solid black;">4</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Angles</td>
 +
<td style="border:1pt solid black;">3X3X3cm</td>
 +
<td style="border:1pt solid black;">18</td>
 +
<td style="border:1pt solid black;">0.41</td>
 +
<td style="border:1pt solid black;">0.45</td>
 +
<td style="border:1pt solid black;">7.38</td>
 +
<td style="border:1pt solid black;">8.1</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Hinges</td>
 +
<td style="border:1pt solid black;">4X2.5cm</td>
 +
<td style="border:1pt solid black;">18</td>
 +
<td style="border:1pt solid black;">0.82</td>
 +
<td style="border:1pt solid black;">0.90</td>
 +
<td style="border:1pt solid black;">14.76</td>
 +
<td style="border:1pt solid black;">16.2</td>
 +
</tr>
 +
 
 +
<tr >
 +
<td style="border:1pt solid black;">Door Knob</td>
 +
<td style="border:1pt solid black;">-</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">2.39</td>
 +
<td style="border:1pt solid black;">2.62</td>
 +
<td style="border:1pt solid black;">2.39</td>
 +
<td style="border:1pt solid black;">2.62</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Long sleeve gloves</td>
 +
<td style="border:1pt solid black;">-</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.69</td>
 +
<td style="border:1pt solid black;">1.85</td>
 +
<td style="border:1pt solid black;">1.69</td>
 +
<td style="border:1pt solid black;">1.85</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Adhesive</td>
 +
<td style="border:1pt solid black;">-</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.05</td>
 +
<td style="border:1pt solid black;">1.15</td>
 +
<td style="border:1pt solid black;">1.05</td>
 +
<td style="border:1pt solid black;">1.15</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Cable ties and Mounts</td>
 +
<td style="border:1pt solid black;">1.9X1.9cm</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">2.29</td>
 +
<td style="border:1pt solid black;">2.51</td>
 +
<td style="border:1pt solid black;">2.29</td>
 +
<td style="border:1pt solid black;">2.51</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Foam Rubber sheet 3mm</td>
 +
<td style="border:1pt solid black;">10X60cm</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.13</td>
 +
<td style="border:1pt solid black;">1.24</td>
 +
<td style="border:1pt solid black;">1.13</td>
 +
<td style="border:1pt solid black;">1.24</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Foam rubber Sheet 6mm</td>
 +
<td style="border:1pt solid black;">30X30cm</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.71</td>
 +
<td style="border:1pt solid black;">1.88</td>
 +
<td style="border:1pt solid black;">1.71</td>
 +
<td style="border:1pt solid black;"> 1.88</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Plastic Sheet</td>
 +
<td style="border:1pt solid black;">50X100cm</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.25</td>
 +
<td style="border:1pt solid black;">1.37</td>
 +
<td style="border:1pt solid black;">1.25</td>
 +
<td style="border:1pt solid black;">1.37</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;"><a href="http://www.uvps.com/product.asp?code=FILTER+++B"><span style="color:#0000EE">UV foil</span></a></td>
 +
<td style="border:1pt solid black;">blocks &lambda;<540 nm,<br>50X30cm</br></td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">3.87</td>
 +
<td style="border:1pt solid black;">4.25</td>
 +
<td style="border:1pt solid black;">3.87</td>
 +
<td style="border:1pt solid black;">4.25</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Neodymium cube magnets</td>
 +
<td style="border:1pt solid black;">4X4X4mm</td>
 +
<td style="border:1pt solid black;">2</td>
 +
<td style="border:1pt solid black;">0.29</td>
 +
<td style="border:1pt solid black;">0.32</td>
 +
<td style="border:1pt solid black;">0.58</td>
 +
<td style="border:1pt solid black;">0.64</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;"><a href="http://www.miniinthebox.com/5m-300x3528-smd-warm-white-red-green-blue-yellow-led-strip-light-dc12v_p2256338.html?category_id=4653&prm=2.4.1.1"><span style="color:#0000EE">LED Strips</span></a></td>
 +
<td style="border:1pt solid black;">625 nm, 150 cm, <br>12V, 2.7W</br></td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.47</td>
 +
<td style="border:1pt solid black;">1.61</td>
 +
<td style="border:1pt solid black;">1.47</td>
 +
<td style="border:1pt solid black;">1.61</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;"><a href="http://www.mouser.de/ProductDetail/CUI/SWI6-12-E-P5/?qs=sGAEpiMZZMtz8P%2feuiupSRmvr4QPIAOoyU1Pml94Egk%3d"><span style="color:#0000EE">Regulated Power Supply</span></a></td>
 +
<td style="border:1pt solid black;">12V, 600mA</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">9.35</td>
 +
<td style="border:1pt solid black;"> 10.26</td>
 +
<td style="border:1pt solid black;">9.35</td>
 +
<td style="border:1pt solid black;">10.26</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="border:1pt solid black;">Switch</td>
 +
<td style="border:1pt solid black;">1A</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.10</td>
 +
<td style="border:1pt solid black;">1</td>
 +
<td style="border:1pt solid black;">1.10</td>
 +
</tr>
 +
 
 +
<tr>
 +
<th style="border:1pt solid black;background-color:#7fb983;">Total<sup>*</sup> </th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"></th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"></th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"></th>
 +
<th style="border:1pt solid black;background-color:#7fb983;"></th>
 +
<th style="border:1pt solid black;background-color:#7fb983;">184.47</th>
 +
<th style="border:1pt solid black;background-color:#7fb983;">202.37</th>
 +
</tr>
 +
</table>
 +
<span style="font-size:10px; margin-left:85px"><sup>*</sup>As on 16<sup>th</sup> October 2016</span>
 +
<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 
<h2 style="border-bottom: 5px solid #005b04;padding-left: 1.0cm;">Assembly</h2>
 
<h2 style="border-bottom: 5px solid #005b04;padding-left: 1.0cm;">Assembly</h2>
 
<br/>
 
<br/>

Revision as of 15:33, 19 October 2016

Welcome to iGEM Aachen 2016

Integrated Practices

We have also taken into account the needs and thoughts of the public to improve our project.

Dark Bench

Idea


Our project forced us to grapple with the inconvenience of having to handle chemicals that are destroyed, when exposed to too much light. The laboratory, that was kindly made available for us, is located in a building with a glass front and many people work there with us. Working protected from light without causing us or others inconveniences seemed impossible. That is how the idea for the “Dark Bench” emerged.
The most important questions we asked ourselves and others working with light sensitive materials were: Does our design really constitute an advantage opposed to the usual solutions like aluminum foil and cardboard? And: Can we make it affordable?

Design


The Dark bench is a light controlled space which facilitates the handling of the photo-sensitive caged amino acids. It is entirely made using laser-cut parts and readily available resources to make it a Do-It-Yourself and inexpensive device. As all the parts are assembled with help of angles and hinges, the Dark bench can be dismantled and assembled with little effort.
It´s features comprise:

  1. Convenient hand hole design
  2. Sliding drawer
  3. Optical window/UV protection window
  4. Safelight

Click for detailed information.

Cost Calculation


“Or, if someone else built it for you, how much would it cost.”
Dr. David Mishler, Austin, Texas

That was Dr. Mishlers concern, when he gave us some feedback to the design of our model. At first he seemed to be interested in our Hardware part, but was skeptical if having the advantages would be worth the price. We estimated the costs to build one darkbench at about 200 $.

Detailed calculation.

Component Specifications Quantity Costs*
[€/piece]
 Costs*
[$/piece]
 Final*
[€]
 Final*
[$]
Opaque plexiglas 4mm 50X100 cm 5 22.45 24.63 112.25 123.15
Transparent plexiglas 3mm 25X50 cm 1 7.5 8.23 7.5 8.23
Screws M3X10 155 0.05 0.05 7.75 7.75
Screws M3X16 10 0.08 0.09 0.8 0.9
Screws M3X20 25 0.09 0.10 2.25 2.5
Washer 3.2mm 200 0.02 0.02 4 4
Angles 3X3X3cm 18 0.41 0.45 7.38 8.1
Hinges 4X2.5cm 18 0.82 0.90 14.76 16.2
Door Knob - 1 2.39 2.62 2.39 2.62
Long sleeve gloves - 1 1.69 1.85 1.69 1.85
Adhesive - 1 1.05 1.15 1.05 1.15
Cable ties and Mounts 1.9X1.9cm 1 2.29 2.51 2.29 2.51
Foam Rubber sheet 3mm 10X60cm 1 1.13 1.24 1.13 1.24
Foam rubber Sheet 6mm 30X30cm 1 1.71 1.88 1.71 1.88
Plastic Sheet 50X100cm 1 1.25 1.37 1.25 1.37
UV foil blocks λ<540 nm,
50X30cm
 1 3.87 4.25 3.87 4.25
Neodymium cube magnets 4X4X4mm 2 0.29 0.32 0.58 0.64
LED Strips 625 nm, 150 cm,
12V, 2.7W
 1 1.47 1.61 1.47 1.61
Regulated Power Supply 12V, 600mA 1 9.35 10.26 9.35 10.26
Switch 1A 1 1 1.10 1 1.10
Total* 184.47 202.37
*As on 16th October 2016

Assembly


“I think it could make work with photosensitive substances easier. The questions what is the inconvenience to building the box versus the added convenience of using it.”
Dr. David Mishler, Austin, Texas

LIPs Stick
Steps to build your own LIPs Stick

Conclusion


We are very content with the design of our “Dark Bench”. We think it is convenient to work with and can help avoid production of unnecessary waste.
The manual for building the “Dark Bench” can be downloaded here.


Link to our website
Wiki 2014 Aachen
Wiki 2015 Aachen
igem@rwth-aachen.de