Difference between revisions of "Team:Exeter/Interlab"
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<p id="pp"> The protocol was improved by using a latin rectangle arrangement in another 96 well plate. A lab robot was used to pipette out the corresponding cultures to their wells according to our preset programme linked to a descrambling spreadsheet. The Latin rectangle is designed so that no sample gets pipetted more than once in any row or column. The order in which the samples get arranged was worked out using the random number function of Microsoft Excel. Sterile LB was loaded into peripheral wells to lessen edge effects which can skew results due to high evaporation at the periphery. These adaptations aim to provide more accurate data by reducing plate effects, samples are spread randomly around the plate so as to minimise differences in results due to irregularities in plate temperature.</p> | <p id="pp"> The protocol was improved by using a latin rectangle arrangement in another 96 well plate. A lab robot was used to pipette out the corresponding cultures to their wells according to our preset programme linked to a descrambling spreadsheet. The Latin rectangle is designed so that no sample gets pipetted more than once in any row or column. The order in which the samples get arranged was worked out using the random number function of Microsoft Excel. Sterile LB was loaded into peripheral wells to lessen edge effects which can skew results due to high evaporation at the periphery. These adaptations aim to provide more accurate data by reducing plate effects, samples are spread randomly around the plate so as to minimise differences in results due to irregularities in plate temperature.</p> | ||
| − | <p id="pp">The measurements were taken using the same calibration settings as before on the Tecan plate reader every hour for 6 hours. The plate was placed into mini shaking incubator at 37°C between plate readings. A scratch was noticed on the plate lid and was replaced. This lid was cold compared to the incubated plate and so condensation formed this could have affected the results. Placed in incubator briefly to warm up. The results were complied into graphs using the iGEM Interlab excel document</p> | + | <p id="pp">The measurements were taken using the same calibration settings as before on the Tecan plate reader every hour for 6 hours. The plate was placed into mini shaking incubator at 37°C between plate readings. A scratch was noticed on the plate lid and was replaced. This lid was cold compared to the incubated plate and so condensation formed this could have affected the results. Placed in incubator briefly to warm up. The results were complied into graphs using the iGEM Interlab excel document.</p> |
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<div class="col-xs-6" style="padding:5px 2% 5px 10%;margin:0;"> | <div class="col-xs-6" style="padding:5px 2% 5px 10%;margin:0;"> | ||
<img src="https://static.igem.org/mediawiki/2016/1/1b/T--Exeter--interlabgraphforpart1egl.png" | <img src="https://static.igem.org/mediawiki/2016/1/1b/T--Exeter--interlabgraphforpart1egl.png" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
| − | <span class="caption">Fig. 3</span> | + | <span class="caption">Fig. 3 Fluoresce/Abs 600 measurements using iGEM Interlab layout</span> |
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<img src="https://static.igem.org/mediawiki/2016/a/a6/T--Exeter--latinrectangleinterlabgraphforpart1egl.png" | <img src="https://static.igem.org/mediawiki/2016/a/a6/T--Exeter--latinrectangleinterlabgraphforpart1egl.png" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
| − | <span class="caption">Fig. 4</span> | + | <span class="caption">Fig. 4 Fluoresce/Abs 600 measurements using Latin Rectangle layout</span> |
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Revision as of 15:44, 19 October 2016
