Difference between revisions of "Team:Peshawar/Parts"

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{{Peshawar}}
 
 
<html>
 
<html>
  
 +
<head>
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    <link rel="icon" type="image/png" href="https://static.igem.org/mediawiki/2016/f/f5/T-Peshawar--favicon-v1-compressor.png">
 +
    <title>Parts - iGEM Peshawar 2016</title>
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:Peshawar/CSS?action=raw&ctype=text/css" />
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</head>
  
 +
<body>
 +
    <div class="outermost-box">
 +
        <div id="menu-button" class="floating-button-wrapper">
 +
    <div id="menu-background" class="js-toggle-left-slidebar">
 +
    </div>
 +
</div>
 +
<!--End of floating menu button wrapper -->        <div off-canvas="slidebar-1 left reveal">
 +
    <nav>
 +
        <ul class="navigation-list">
 +
            <li><a href="https://2016.igem.org/Team:Peshawar">Home</a></li>
 +
            <hr>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Description">Project</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Model">Modeling</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Notebook">Notebook</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Safety">Safety</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Parts">Parts</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Basic_Part">Basic Part</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Composite_Part">Composite Part</a></li>
 +
            <hr>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Team">Team</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Attributions">Attributions</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Collaborations">Collaborations</a></li>
 +
            <hr>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Human_Practices">Human Practices</a></li>
 +
            <li>
 +
                <a href="https://2016.igem.org/Team:Peshawar/HP/Silver">Silver</a>
 +
                <span>  |  </span>
 +
                <a href="https://2016.igem.org/Team:Peshawar/HP/Gold">Gold</a>
 +
            </li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Integrated_Practices">Integrated Practices</a></li>
 +
            <hr>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Engagement">Public Engagement</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Awards">Awards</a></li>
 +
            <hr>
 +
            <li><a href="https://2016.igem.org/Team:Peshawar/Wiki">How to design an iGEM Wiki</a></li>
  
 +
        </ul>
 +
    </nav>
 +
    <article id="social-main-footer">
 +
    <!--FACEBOOK-->
 +
    <a href="https://www.facebook.com/iGEMPeshawar2016/" target="_blank">
 +
        <svg class="social-main-footer-svg facebook" width="61" height="60" viewBox="0 0 61 60" xmlns="http://www.w3.org/2000/svg">
 +
            <title>
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                T--Peshawar--facebook-round-v2
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            </title>
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            <g id="Page-1" fill="none" fill-rule="evenodd">
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                <g id="T--Peshawar--facebook-round-v2" fill="#808184">
 +
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 +
                </g>
 +
            </g>
 +
        </svg>
 +
    </a>
 +
    <!--TWITTER-->
 +
    <a href="https://twitter.com/igem_peshawar" target="_blank">
 +
        <svg class="social-main-footer-svg twitter" width="60" height="60" viewBox="0 0 60 60" xmlns="http://www.w3.org/2000/svg">
 +
            <title>
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                T--Peshawar--twitter-round-v2
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            </title>
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            <g id="Page-1" fill="none" fill-rule="evenodd">
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                <g id="T--Peshawar--twitter-round-v2" fill="#808184">
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 +
                    <path d="M49.642 21.783c-1.325.587-2.75.984-4.242 1.163 1.527-.914 2.698-2.36 3.247-4.085-1.428.848-3.006 1.46-4.692 1.79-1.343-1.433-3.265-2.33-5.39-2.33-4.077 0-7.384 3.308-7.384 7.385 0 .58.065 1.142.19 1.683-6.134-.31-11.576-3.247-15.222-7.718-.636 1.093-.998 2.362-.998 3.715 0 2.56 1.303 4.82 3.283 6.148-1.208-.038-2.35-.373-3.347-.923v.09c0 3.58 2.547 6.566 5.927 7.242-.62.174-1.27.26-1.946.26-.476 0-.94-.044-1.39-.13.94 2.933 3.666 5.07 6.9 5.126-2.53 1.982-5.715 3.163-9.173 3.163-.596 0-1.185-.032-1.762-.1 3.27 2.093 7.15 3.316 11.32 3.316 13.59 0 21.015-11.25 21.015-21.013 0-.32-.006-.64-.02-.955 1.444-1.043 2.7-2.344 3.685-3.824" id="twitter" />
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                </g>
 +
            </g>
 +
        </svg>
 +
    </a>
 +
    <!--INSTAGRAM-->
 +
    <a href="https://www.instagram.com/igempeshawar/" target="_blank">
 +
        <svg class="social-main-footer-svg instagram" width="61" height="61" viewBox="0 0 61 61" xmlns="http://www.w3.org/2000/svg">
 +
            <title>
 +
                T--Peshawar--instagram-round-v2
 +
            </title>
 +
            <g id="Page-1" fill="none" fill-rule="evenodd">
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                <g id="T--Peshawar--instagram-round-v2" fill="#808184">
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                    <path d="M45.3 42.212c0 1.902-1.548 3.446-3.45 3.446H19.763c-1.903 0-3.45-1.544-3.45-3.446v-14.73h5.375c-.464 1.144-.727 2.39-.727 3.695 0 5.426 4.417 9.837 9.845 9.837 5.428 0 9.843-4.41 9.843-9.837 0-1.306-.264-2.55-.725-3.694H45.3v14.73zm-19.63-14.73c1.15-1.593 3.022-2.64 5.137-2.64 2.115 0 3.985 1.047 5.136 2.64.75 1.042 1.203 2.314 1.203 3.695 0 3.492-2.845 6.33-6.34 6.33-3.494 0-6.336-2.838-6.336-6.33 0-1.38.45-2.653 1.2-3.694zm18.174-10.145l.795-.004v6.097l-6.083.022-.022-6.098 5.308-.017zM41.85 13.19H19.763c-3.835 0-6.957 3.118-6.957 6.952v22.07c0 3.834 3.122 6.952 6.957 6.952H41.85c3.834 0 6.957-3.118 6.957-6.952v-22.07c0-3.834-3.123-6.952-6.958-6.952z" id="instagram" />
 +
                </g>
 +
            </g>
 +
        </svg>
 +
    </a>
 +
    <!--BLOG-->
 +
    <a href="http://www.synbiokp.com/blog/" target="_blank">
 +
        <svg class="social-main-footer-svg blog" width="60" height="60" viewBox="0 0 60 60" xmlns="http://www.w3.org/2000/svg">
 +
            <title>
 +
                T--Peshawar--blog-round-v2
 +
            </title>
 +
            <g id="Page-1" fill="none" fill-rule="evenodd">
 +
                <g id="T--Peshawar--blog-round-v2" fill="#808184">
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                    <path d="M34.95 19.916c7.654 0 14 6.357 14 14.025 0 7.64-6.375 14.03-14 14.03h-7.942C19.393 47.968 13 41.57 13 33.94V15.22c0-1.78 1.47-3.255 3.25-3.255 1.768 0 3.247 1.482 3.247 3.256 0 6.24.01 12.48.01 18.72 0 4.11 3.4 7.52 7.5 7.52h7.943c4.084 0 7.504-3.424 7.504-7.514 0-4.108-3.403-7.516-7.504-7.516h-8.568c-1.777 0-3.248-1.475-3.248-3.255 0-1.773 1.477-3.254 3.248-3.254h8.568z" id="blog" />
 +
                </g>
 +
            </g>
 +
        </svg>
 +
    </a>
 +
</article>
 +
</div>
 +
<!--End of off-canvas menu-->        <div canvas="container">
 +
            <section class="inner-container">
 +
                <div id="header-oc" class="header-outer-container">
 +
    <div class="sticky-header">
 +
        <div class="logo-wrapper">
 +
            <a href="https://2016.igem.org/Team:Peshawar">
 +
                <img id="id-logo" class="logo" src="https://static.igem.org/mediawiki/2016/7/7b/T--Peshawar--iGEM-Peshawar-Logo---New-Pakol-v2-2.png">
 +
            </a>                           
 +
        </div>
 +
    </div>
 +
</div>
 +
<!--End of full width header containing responsive logo  -->                <script type="text/javascript">
  
 +
    var button = document.getElementById('menu-button');
 +
    button.style.position = "fixed";
 +
    button.style.zIndex = "-1";
 +
    var buttonImage = document.getElementById("menu-background");
  
 +
    //Wait for the Logo to load and fill he div
 +
    document.getElementById('id-logo').onload = function() {
 +
        var header = document.getElementById('header-oc');
 +
        headerHeight = header.scrollHeight;
 +
        screenWidth = header.scrollWidth;
 +
        console.log('headerHeight',headerHeight, 'headerwidth', screenWidth);
  
<div class="column full_size">
+
        //Scale the button according to header height
 +
        var buttonheight = headerHeight / 3.5;
 +
        buttonImage.style.backgroundSize = buttonheight + 'px';
 +
        buttonImage.style.height = buttonheight +'px';
 +
        buttonImage.style.width = buttonheight*1.25 +'px';
 +
        button.style.zIndex = "2";
  
 +
        // Calculate button coordinates
 +
        menuButtonHeight = 80;                       
 +
        var buttonTopPosition = headerHeight / 2 - buttonheight / 2;
 +
        var buttonLeftPosition = screenWidth / 36 ;
  
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
        // Poistion the button at optimal location
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
        button.style.left = buttonLeftPosition + 'px';
 +
        button.style.top = buttonTopPosition + 'px';
 +
    };
  
 +
    // Hack for menu button not appear when the back button is pressed in history
 +
    window.onpageshow = function(event) {
 +
        var button = document.getElementById('menu-button');
 +
        button.style.position = "fixed";
 +
        button.style.zIndex = "-1";
 +
        var buttonImage = document.getElementById("menu-background");
 +
       
 +
        var header = document.getElementById('header-oc');
 +
        headerHeight = header.scrollHeight;
 +
        screenWidth = header.scrollWidth;
 +
        console.log('headerHeight',headerHeight, 'headerwidth', screenWidth);
  
</div>
+
        //Scale the button according to header height
 +
        var buttonheight = headerHeight / 3.5;
 +
        buttonImage.style.backgroundSize = buttonheight + 'px';
 +
        buttonImage.style.height = buttonheight +'px';
 +
        buttonImage.style.width = buttonheight*1.25 +'px';
 +
        button.style.zIndex = "2";
  
 +
        // Calculate button coordinates
 +
        menuButtonHeight = 80;                       
 +
        var buttonTopPosition = headerHeight / 2 - buttonheight / 2;
 +
        var buttonLeftPosition = screenWidth / 36 ;
  
 +
        // Poistion the button at optimal location
 +
        button.style.left = buttonLeftPosition + 'px';
 +
        button.style.top = buttonTopPosition + 'px';
 +
    };
  
 +
</script>
  
  
<div class="column half_size">
 
<div class="highlight">
 
<h5>Note</h5>
 
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
</div>
 
</div>
 
  
  
  
  
<div class="column half_size">
+
                <div class="centered-content-wrapper-container">
 +
                    <div class="centered-content-wrapper">
 +
                    <h2>Parts Summary</h2>
 +
                    <p>Over the course of this summer, we have created 6 new parts, out of which 2 are basic and 4 are composite. Almost all of our parts have been synthesized using IDT's extremely generous free synthesis offer. We also improved 2 previous parts from the registry &mdash; BBa_K248001 and K248002 &mdash; by codon optimization and removing restricted sites from their sequence. We then submitted BBa_K2056001 and BBa_K2056002 as improved parts. </p>
 +
                    </div>
 +
                    <!--End of centered-content-wrapper-->
 +
                </div>
 +
                <!--End of centered-content-wrapper-container-->
  
<h5>Adding parts to the registry</h5>
 
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
</div>
 
  
 +
                <div class="full-content-wrapper-container">
 +
                    <div class="full-width-content-wrapper">
 +
                        <table class="table-minimal">
 +
                            <thead>
 +
                                <tr>
 +
                                    <th>Prize</th>
 +
                                    <th>Medal</th>
 +
                                    <th>Part Name</th>
 +
                                    <th>Type</th>
 +
                                    <th>Description</th>
 +
                                    <th>Designer</th>
 +
                                    <th>Length</th>
 +
                                    <th></th>
  
 +
                                </tr>
 +
                            </thead>
 +
                            <tbody>
 +
                                <tr>
 +
                                    <td></td>
 +
                                    <td>Silver </td>
 +
                                    <td><a href="http://parts.igem.org/Part:BBa_K2056001">BBa_K2056001</a></td>
 +
                                    <td>Basic</td>
 +
                                    <td>NsrR - NO(x) sensitive pYear, pNirk repressor protein</td>
 +
                                    <td>Muhammad Ismail</td>
 +
                                    <td>426bp</td>
 +
                                    <td></td>
  
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>Best Basic Part</td>
 +
                                    <td>Bronze </td>
 +
                                    <td><a href="http://parts.igem.org/Part:BBa_K2056002">BBa_K2056002</a></td>
 +
                                    <td>Basic</td>
 +
                                    <td>pNirK &mdash; promoter region 'nirK' (nitrite reductase)</td>
 +
                                    <td>Muhammad Ismail</td>
 +
                                    <td>312bp</td>
 +
                                    <td><span class="pink-cell" >.</span></td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td></td>
 +
                                    <td>Silver </td>
 +
                                    <td><a href="http://parts.igem.org/Part:BBa_K2056000">BBa_K2056000</a></td>
 +
                                    <td>Composite</td>
 +
                                    <td>Constitutive promoter(J23110), RBS(B0034), NsrR(K2056001), Double terminator(B0015)</td>
 +
                                    <td>Muhammad Ismail</td>
 +
                                    <td>626bp</td>
 +
                                    <td><span class="pink-cell" >.</span></td>
  
 +
                                </tr>
 +
                                <tr>
 +
                                    <td></td>
 +
                                    <td>Silver </td>
 +
                                    <td><a href="http://parts.igem.org/Part:BBa_K2056003">BBa_K2056003</a></td>
 +
                                    <td>Composite</td>
 +
                                    <td>Carbon Monoxide sensor device (pCooF-Blue Chromoprotein)</td>
 +
                                    <td>Muhammad Ismail</td>
 +
                                    <td>1748bp</td>
 +
                                    <td><span class="pink-cell" >.</span></td>
  
<div class="column half_size">
+
                                </tr>
 +
                                <tr>
 +
                                    <td></td>
 +
                                    <td>Silver </td>
 +
                                    <td><a href="http://parts.igem.org/Part:BBa_K2056004">BBa_K2056004</a></td>
 +
                                    <td>Composite</td>
 +
                                    <td>NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein)</td>
 +
                                    <td>Muhammad Ismail</td>
 +
                                    <td>1770bp</td>
 +
                                    <td></td>
  
<h5>What information do I need to start putting my parts on the Registry?</h5>
+
                                </tr>
<p>The information needed to initially create a part on the Registry is:</p>
+
                                <tr>
<ul>
+
                                    <td>Best Composite part</td>
<li>Part Name</li>
+
                                    <td>Silver</td>
<li>Part type</li>
+
                                    <td><a href="http://parts.igem.org/Part:BBa_K2056005">BBa_K2056005</a></td>
<li>Creator</li>
+
                                    <td>Composite</td>
<li>Sequence</li>
+
                                    <td>NOx(Oxides of Nitrogen) sensor device (pYeaR-Yellow chromoprotein)</td>
<li>Short Description (60 characters on what the DNA does)</li>
+
                                    <td>Muhammad Ismail</td>
<li>Long Description (Longer description of what the DNA does)</li>
+
                                    <td>1558bp</td>
<li>Design considerations</li>
+
                                    <td><span class="pink-cell" >.</span></td>
</ul>
+
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                        <p class='pink-text'>Pink denotes favorite parts. </p>
 +
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<p>
 
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
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        <h4><img id="location" src="https://static.igem.org/mediawiki/2016/7/7c/T--Peshawar--location-compressor.png">  Institute of Integrative Biosciences,
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
        <h3>Our Partners</h3>
 
+
        <br>
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
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            <a href="http://kp.gov.pk/" target="_blank">
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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                <img src="https://static.igem.org/mediawiki/2016/a/a1/T--Peshawar--govt-of-kpk-logo-small-gs-compressed.png">
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
            </a>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
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<h5>Part Table </h5>
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Revision as of 16:37, 19 October 2016

Parts - iGEM Peshawar 2016

Parts Summary

Over the course of this summer, we have created 6 new parts, out of which 2 are basic and 4 are composite. Almost all of our parts have been synthesized using IDT's extremely generous free synthesis offer. We also improved 2 previous parts from the registry — BBa_K248001 and K248002 — by codon optimization and removing restricted sites from their sequence. We then submitted BBa_K2056001 and BBa_K2056002 as improved parts.

Prize Medal Part Name Type Description Designer Length
Silver BBa_K2056001 Basic NsrR - NO(x) sensitive pYear, pNirk repressor protein Muhammad Ismail 426bp
Best Basic Part Bronze BBa_K2056002 Basic pNirK — promoter region 'nirK' (nitrite reductase) Muhammad Ismail 312bp .
Silver BBa_K2056000 Composite Constitutive promoter(J23110), RBS(B0034), NsrR(K2056001), Double terminator(B0015) Muhammad Ismail 626bp .
Silver BBa_K2056003 Composite Carbon Monoxide sensor device (pCooF-Blue Chromoprotein) Muhammad Ismail 1748bp .
Silver BBa_K2056004 Composite NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein) Muhammad Ismail 1770bp
Best Composite part Silver BBa_K2056005 Composite NOx(Oxides of Nitrogen) sensor device (pYeaR-Yellow chromoprotein) Muhammad Ismail 1558bp .

Pink denotes favorite parts.