Difference between revisions of "Team:UCL/Demonstrate"

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                     <div class="col-md-8 col-md-offset-2 ess-template-general animate-box"><h2> <center> <b> <u> IrrE biobrick for lubricant </b> </u> </center> </h2>
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                         <h4> IrrE
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This construct already exists in the registry, however, we wanted to expand the pre-existing functionality. Here we have shown that this gene goes beyond helping growth in high salt concentration solutions by demonstrating that it actually exists bacterial growth in lubricant.
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To achieve this we used lubricant from the market, specifically, Superdrug to demonstrate this in order to prove the gene would be functional in real-world conditions.
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Our results indicate our proof of concept that under simulated conditions to be a success. This is because E. coli transformed with IrrE had improved growth in LB and lubricant compared to wild type.
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We believe this is the first step to demonstrate that a living organism would be capable of living in lubricant.
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<PHOTO OF LUBRICANT>
 
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Revision as of 16:40, 19 October 2016

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UCL iGEM 2016 | BioSynthAge

DEMONSTRATE

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OVERVIEW

Talking to Aubrey de Grey and Filipe has stressed the need for us to be able to explore through not just conceptional design but through wet lab, how we can prove that our ideas will work in the real world, in the complex human body. Real world conditions to us is defined as the human body. How will our biobricks interact under real conditions? Will our biobrick survive in the human body? Will they interact or affect other processes in the body? How can we then ensure that the result of this exploration can feed back onto the design of our devices to make them safer and more controlled.

IrrE biobrick for lubricant

IrrE This construct already exists in the registry, however, we wanted to expand the pre-existing functionality. Here we have shown that this gene goes beyond helping growth in high salt concentration solutions by demonstrating that it actually exists bacterial growth in lubricant. To achieve this we used lubricant from the market, specifically, Superdrug to demonstrate this in order to prove the gene would be functional in real-world conditions. Our results indicate our proof of concept that under simulated conditions to be a success. This is because E. coli transformed with IrrE had improved growth in LB and lubricant compared to wild type. We believe this is the first step to demonstrate that a living organism would be capable of living in lubricant.