Difference between revisions of "Team:Aix-Marseille/Design"

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* adsorb ions on bacterial flagella protein
 
* adsorb ions on bacterial flagella protein
 
* the ambient reducer power reduces ions into oxidized nano particles
 
* the ambient reducer power reduces ions into oxidized nano particles
All together, these findings incite us to use these natural properties to build a biobricks which is a high affinity binder of platinum based on <i>E. coli</i> and <i>Desulfovibrio vulgaris</i> flagellum and synthetic peptides sequences. To this end, we analyze the flagella sequence and structural proprieties of the external part of the flagella. Then, on the part of the flagellin faced to the external medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorption specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production.
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All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinum based on <i>E. coli</i> and <i>Desulfovibrio vulgaris</i> flagellum and synthetic peptides. To this end, we analyze the flagella sequences and structural properties of the external part of the flagella. Then, on the part of the flagellin faced to the external medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorption specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production.
 
[[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]]
 
[[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]]
 
Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions of the medium and reduce them into oxydised nanoparticules usable in industry. A simple centrifugation of the flagellin binding to the platinum allow to concentrate the metal a second time.
 
Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions of the medium and reduce them into oxydised nanoparticules usable in industry. A simple centrifugation of the flagellin binding to the platinum allow to concentrate the metal a second time.

Revision as of 16:46, 19 October 2016