Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-RFP"

 
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<div class="technology">7/10</div>
 
<div class="technology">7/10</div>
 
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<div class="thelanguage">
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<li><p style="font-size: 18px;">BBa_E1010 extracted the checked with restriction digestion (EocRI and PstI  747bp and 2029bp)</p></li>
+
<li><p style="font-size: 18px;">BBa_E1010 extracted plasmid and checked with restriction digestion (EocRI and PstI  747bp and 2029bp)</p></li>
  
 
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<div class="technology">8/1</div>
 
<div class="technology">8/1</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">RFP checked with PCR</p></li>
+
<li><p style="font-size: 18px;">RFP checked with KOD PCR</p></li>
  
 
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<div class="technology">8/3</div>
 
<div class="technology">8/3</div>
 
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<div class="thelanguage">
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<li><p style="font-size: 18px;">RFP digested with EcoRI and BamHI for ligation</p></li>
+
<li><p style="font-size: 18px;">RFP digested with EcoRI and BamHI for the ligation with C120_empty</p></li>
  
 
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Latest revision as of 17:07, 19 October 2016

RFP

RFP


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7/9

  • BBa_E1010 T-streaked onto agar plates

    • [Collapse]

    7/10

  • BBa_E1010 extracted plasmid and checked with restriction digestion (EocRI and PstI 747bp and 2029bp)

    • [Collapse]

    7/16

  • BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)

    • [Collapse]

    7/17

  • BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)

    • [Collapse]

    7/18

  • BBa_E1010 checked with plasmid PCR with newly design primers

    • [Collapse]

    7/19

  • BBa_E1010 checked with plasmid PCR with the addition of Mg2+

    • [Collapse]

    7/22

  • BBa_E1010 checked with Taq polymerase plasmid PCR (successful)

    • [Collapse]

    7/25

  • BBa_E1010 amplified with KOD polymerase PCR

    • [Collapse]

    7/26

  • RFP amplified with KOD polymerase PCR

  • RFP digested with EcoRI and BamHI

    • [Collapse]

    7/29

  • pBAR_RFP checked with KOD polymerase PCR (contaminated NC)

    • [Collapse]

    8/1

  • RFP checked with KOD PCR

    • [Collapse]

    8/3

  • RFP digested with EcoRI and BamHI for the ligation with C120_empty

    • [Collapse]