Difference between revisions of "Team:IIT Kharagpur/Parts"

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<h1 class="well-come" style="font-family:Dosis;">PARTS</h1>
 
<h1 class="well-come" style="font-family:Dosis;">PARTS</h1>
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<!-- <div class="col-md-8 col-md-offset-2 tabledown">
 
<p class="intro-message" style="font-family:Dosis; font-size:20px; text-align:center;">HARD WORK JUST LIKE JUST MA AND PA TAUGHT US</p>
 
<p class="intro-message" style="font-family:Dosis; font-size:20px; text-align:center;">HARD WORK JUST LIKE JUST MA AND PA TAUGHT US</p>
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Integrating various aspects, iGEM, above all, is a struggle to turn your idea into reality. Ultimately, this implies creating the precious parts. The parts are created by joining biobricks. This year, we have used the standard assembly protocols in order to ligate the various necessary biobricks.
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However, we could not achieve our vision, and this year we will be submitting two intermediate parts, and one composite part.
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         </thead>
 
         </thead>
 
         <tr>
 
         <tr>
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         <td>Cont</td>
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         <td>1.</td>
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         <td>Content</td>
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         <td>
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         <td>Content</td>
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                                BBa_K2163000</td>
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         <td>Content</td>
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         <td>Composite</td>
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         <td>Content</td>
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         <td>CFP+HIV+YFP</td>
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         <td>1499</td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
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         <td>Content</td>
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         <td>2.</td>
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         <td>Content</td>
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         <td>BBa_S05365</td>
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         <td>Content</td>
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         <td>
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         <td>Content</td>
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Intermediate
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         <td>Content</td>
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</td>
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         <td>CFP+HIV</td>
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         <td>773</td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
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         <td>Content</td>
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         <td>3.</td>
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         <td>Content</td>
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         <td>BBa_S05366</td>
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         <td>Content</td>
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         <td>
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         <td>Content</td>
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Intermediate
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         <td>Content</td>
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</td>
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         <td>HIV+YFP</td>
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         <td>768</td>
 
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<h3>Composite Part</h3>
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<h3>PARTS</h3>
 
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<h4><b>1. BBa_1</b></h4>
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<h4><b>1. BBa_K2163000
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This is a composite part, comprising of Cyan Fluorescent Protein (CFP), HIV Aspartyl Protease Specific Cleavage Site, and Yellow Fluorescent Protein (YFP). This part functions as the core of FRET detection system. As long as the cleavage site is intact, the FRET pair is active, while cleavage of the site leads to inactivation of the FRET pair. When attached with OmpA at the C terminal, the entire system is presented on the cell surface.
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</p><br><br><br>
  
 
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<img src="https://static.igem.org/mediawiki/2016/7/7f/T--IIT_Kharagpur--cfp%2Bhiv.png"><br><br><br>
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<img src="https://static.igem.org/mediawiki/2016/7/78/T--IIT_Kharagpur--cfp_hiv_yfp.png"><br><br><br>
 
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<h4><b>2. BBa_2</b></h4>
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<h4><b>2. BBa_S05365
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Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
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This is one of the two sub itted intermediate parts. It consists of Cyan Fluorescent Protein (CFP) and HIV Aspartyl Protease Specific Cleavage Site.  
 +
</p>
 
<br><br><br>
 
<br><br><br>
  
 
</div><br>
 
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<img src="https://static.igem.org/mediawiki/2016/7/7f/T--IIT_Kharagpur--cfp%2Bhiv.png"><br><br><br>
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<img src="https://static.igem.org/mediawiki/2016/d/d9/T--IIT_Kharagpur--cfp_hiv.png"><br><br><br>
 
</div>
 
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<div class="col-lg-7 col-md-7 col-sm-12 justify safety-content">
 
<div class="col-lg-7 col-md-7 col-sm-12 justify safety-content">
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<h4><b>1. BBa_3</b></h4>
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<h4><b>3. BBa_S05366
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</b></h4>
 
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Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p><br><br><br>
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This is the other intermediate part. It is made up of the biobricks coding for HIV Aspartyl Protease Specific Cleavage Site and Yellow Fluorescent Protein (YFP).
 +
</p><br><br><br>
  
 
</div><br>
 
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<img src="https://static.igem.org/mediawiki/2016/7/7f/T--IIT_Kharagpur--cfp%2Bhiv.png"><br><br><br>
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<img src="https://static.igem.org/mediawiki/2016/4/43/T--IIT_Kharagpur--hiv_yfp.png"><br><br><br>
 
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Revision as of 17:39, 19 October 2016

IGEM-IIT Kharagpur- Part

Overview

Integrating various aspects, iGEM, above all, is a struggle to turn your idea into reality. Ultimately, this implies creating the precious parts. The parts are created by joining biobricks. This year, we have used the standard assembly protocols in order to ligate the various necessary biobricks. However, we could not achieve our vision, and this year we will be submitting two intermediate parts, and one composite part.



SL.No. Name/Part No. Type Design Length(bp)
1. BBa_K2163000 Composite CFP+HIV+YFP 1499
2. BBa_S05365 Intermediate CFP+HIV 773
3. BBa_S05366 Intermediate HIV+YFP 768


PARTS

1. BBa_K2163000

This is a composite part, comprising of Cyan Fluorescent Protein (CFP), HIV Aspartyl Protease Specific Cleavage Site, and Yellow Fluorescent Protein (YFP). This part functions as the core of FRET detection system. As long as the cleavage site is intact, the FRET pair is active, while cleavage of the site leads to inactivation of the FRET pair. When attached with OmpA at the C terminal, the entire system is presented on the cell surface.








2. BBa_S05365

This is one of the two sub itted intermediate parts. It consists of Cyan Fluorescent Protein (CFP) and HIV Aspartyl Protease Specific Cleavage Site.








3. BBa_S05366

This is the other intermediate part. It is made up of the biobricks coding for HIV Aspartyl Protease Specific Cleavage Site and Yellow Fluorescent Protein (YFP).