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<div class="menu"> | <div class="menu"> | ||
− | <a class="item" href=" https://2016.igem.org/Team:USTC/Team"> Members </a> | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team"> Members </a> |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Sponsor"> Sponsor </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/AboutUSTC"> About USTC </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Acknowledgements"> Acknowledgements </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Gallery"> Gallery </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Collaborations"> Collaborations </a> | |
</div> | </div> | ||
</div> | </div> | ||
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<div class="header">Team</div> | <div class="header">Team</div> | ||
<div class="menu"> | <div class="menu"> | ||
− | <a class="item" href=" https://2016.igem.org/Team:USTC/Team"> Members </a> | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team"> Members </a> |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Sponsor"> Sponsor </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/AboutUSTC"> About USTC </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Acknowledgements"> Acknowledgements </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Gallery"> Gallery </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Collaborations"> Collaborations </a> | |
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</div> | </div> | ||
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</tbody> | </tbody> | ||
− | <table | + | </table> |
<p><strong>Recorder: Chenyang Li and Weijie Jin</strong> | <p><strong>Recorder: Chenyang Li and Weijie Jin</strong> | ||
<strong>Transformation of plasmid YeWGAP</strong></p> | <strong>Transformation of plasmid YeWGAP</strong></p> |
Latest revision as of 18:21, 19 October 2016
Notebook
Together we stand
Performers
DATE:8.14 Recorder:Chenyang Li and Weijie Jin Double Digestion of BD-PYW, AD-PYW-S1, BD-PYW-S1, AD-PYW, AD-PYU and AD-PYU
Procedure:
number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
---|---|---|---|---|---|---|---|---|---|
sample | BD-PYW1001 | AD-PYW-S11002 | BD-PYW-S11003 | AD-PYW1004 | BD-PYW1005 | BD-PYW1006 | AD-PYU1007 | AD-PYU1008 | BD-PYW1009 |
nuclease-free water(μL) | 6.5 | 3.5 | 4 | 2 | 0 | 1.5 | 5 | 1.5 | 0 |
10*Green Buffer(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
plasmid(μL) | 1.5 | 4.5 | 4.0 | 6 | 8 | 6.5 | 3 | 6.5 | 8 |
EcoRI(μL) | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 |
PstI(μL) | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
Number | 1 | 2 | 3 | 4 | |||
---|---|---|---|---|---|---|---|
A260/A280 | 1.88 | 1.86 | 1.88 | 1.84 | |||
concentration(ng/μL) | 741.3 | 639.8 | 655.6 | 602.9 |
sample | 1,2,3,4 | ||||||
---|---|---|---|---|---|---|---|
Sterilized ddH2O | 9 μL | ||||||
2×HiFi-PCR Master | 12.5 μL | ||||||
Template(supernatant) | 1.5 μL | ||||||
Primer 1 | 1 μL | ||||||
Primer 2 | 1 μL | ||||||
total | 25 μL |
stage | temperature | time | |||||
---|---|---|---|---|---|---|---|
Pre-Duration | 95 | 10 min | |||||
Duration | 95 | 1 min | |||||
Anneal | 58 | 1 min | |||||
Extend | 72 | 1 min 10s | |||||
Post-Extend | 72 | 10 min | |||||
Final | 4 | -- |
sample | 1,2,3,4 | ||||||
---|---|---|---|---|---|---|---|
Sterilized ddH2O | 9 μL | ||||||
2×HiFi-PCR Master | 12.5 μL | ||||||
Template(bacteria) | 1.5 μL | ||||||
Primer 1 | 1 μL | ||||||
Primer 2 | 1 μL | ||||||
total | 25 μL |
stage | temperature | time | |||||
---|---|---|---|---|---|---|---|
Pre-Duration | 95 | 10 min | |||||
Duration | 95 | 1 min | |||||
Anneal | 58 | 1 min | |||||
Extend | 72 | 1 min 10s | |||||
Post-Extend | 72 | 10 min | |||||
Final | 4 | -- |
sample | 1,2,3,4 | ||||||
---|---|---|---|---|---|---|---|
Sterilized ddH2O | 9.5 μL | ||||||
2×HiFi-PCR Master | 12.5 μL | ||||||
Template(plasmid) | 1 μL | ||||||
Primer 1 | 1 μL | ||||||
Primer 2 | 1 μL | ||||||
total | 25 μL |
stage | temperature | time | |||||
---|---|---|---|---|---|---|---|
Pre-Duration | 95 | 10 min | |||||
Duration | 95 | 1 min | |||||
Anneal | 58 | 1 min | |||||
Extend | 72 | 1 min 10s | |||||
Post-Extend | 72 | 10 min | |||||
Final | 4 | -- |
Number | 1 | 2 | 3 | 4 | 5 | ||
---|---|---|---|---|---|---|---|
A260/A280 | 1.89 | 1.88 | 1.87 | 1.88 | 1.87 | ||
concentration(ng/μL) | 649.4 | 718.8 | 698.2 | 148.0 | 452.7 |
Number | 1 | 2 | 3 | 4 | 5 | ||
---|---|---|---|---|---|---|---|
A260/A280 | 1.86 | 1.86 | 1.87 | 1.87 | 1.81 | ||
A260/A230 | 2.33 | 2.16 | 2.32 | 2.28 | 1.73 | ||
concentration(ng/μL) | 716.2 | 480.6 | 485.1 | 381.5 | 599.9 |
sample | 08151001 | 08151002 | 08151003 | 08151004 | |||
---|---|---|---|---|---|---|---|
Sterilized ddH2O | 8.5μL | 8μL | 8.5μL | 8μL | |||
2×HiFi-PCR Master | 10μL | 10μL | 10μL | 10μL | |||
Template(bacteria) | 0.5 μL | 1μL | 0.5 μL | 0.5 μL | |||
Primer 1 | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL | |||
Primer 2 | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL | |||
total | 20 μL |
stage | temperature | time | |||||
---|---|---|---|---|---|---|---|
Pre-Duration | 95 | 10 min | |||||
Duration | 95 | 50 s | |||||
Anneal | 55 | 1 min | |||||
Extend | 72 | 1 min 20 s | |||||
Post-Extend | 72 | 10 min | |||||
Final | 4 | -- |
sample | 08151005 | 08151006 | 08151007 | 08151008 | |||
---|---|---|---|---|---|---|---|
Sterilized ddH2O | 8.8μL | 8.8μL | 8.8μL | 8.8μL | |||
2×HiFi-PCR Master | 10μL | 10μL | 10μL | 10μL | |||
Template(bacteria) | 0.2μL | 0.2μL | 0.2μL | 0.2μL | |||
Primer 1 | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL | |||
Primer 2 | 0.5 μL | 0.5 μL | 0.5 μL | 0.5 μL | |||
total | 20 μL |
stage | temperature | time | ||||
---|---|---|---|---|---|---|
Pre-Duration | 95 | 10 min | ||||
Duration | 95 | 50 s | ||||
Anneal | 55 | 1 min | ||||
Extend | 72 | 1 min 20 s | ||||
Post-Extend | 72 | 10 min | ||||
Final | 4 | -- |
number | 08151009 | 08151010 | 08151011 | 08151012 | 08151013 | |
---|---|---|---|---|---|---|
nuclease-free water(μL) | 7.2 | 7.2 | 7.2 | 6.7 | 7.2 | |
10*Green Buffer(μL) | 1 | 1 | 1 | 1 | 1 | |
plasmid(μL) | 1 | 1 | 1 | 1.5 | 1 | |
EcoRI(μL) | 0.6 | 0.6 | 0.6 | 0.6 | 0.6 | |
PstI(μL) | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 |
Number | ADU3-1 | ADU3-2 | ADU3-3 | ADU4-1 | ADU4-2 | ADU4-3 |
---|---|---|---|---|---|---|
A260/A280 | 1.89 | 1.91 | 1.90 | 1.94 | 1.87 | 1.92 |
concentration(ng/μL) | 261.8 | 204.8 | 208.6 | 98.5 | 121.4 | 120.9 |
22μL | Sterilized ddH2O | |||||
25μL | 2×raq PCR Master Mix | |||||
1μL | template | |||||
2μL | primer 1 | |||||
2μL | primer 2 | |||||
50μL | total |
stage | temperature | time | ||||
---|---|---|---|---|---|---|
Pre-Duration | 94 | 4 min | ||||
Duration | 94 | 30 s | ||||
Anneal | 60 | 30 s | ||||
Extend | 72 | 45 | ||||
Post-Extend | 72 | 10 min | ||||
Final | 4 | -- |
stage | temperature | time | ||||
---|---|---|---|---|---|---|
Pre-Duration | 95 | 10 min | ||||
Duration | 95 | 50 s | ||||
Anneal | 55 | 1 min | ||||
Extend | 72 | 1 min 20 s | ||||
Post-Extend | 72 | 10 min | ||||
Final | 4 | -- |
Sample | AD1 | AD2 | BD1 | BD2 | ||
---|---|---|---|---|---|---|
A260/A280 | 1.86 | 1.86 | 1.85 | 1.87 | ||
A260/A230 | 2.25 | 2.18 | 2.04 | 2.36 | ||
concentration(ng/μL) | 338.1 | 257.0 | 336.0 | 301.4 |
Sample | AD1 | AD2 | BD1 | BD2 | ||
---|---|---|---|---|---|---|
A260/A280 | 1.88 | 1.87 | 1.87 | 1.87 | ||
concentration(ng/μL) | 197.8 | 184.6 | 273.6 | 262.8 |
Sample | AD1(0228) | AD2 | BD1(0229) | BD2 | PYU1(0230) | PYU2 |
---|---|---|---|---|---|---|
A260/A280 | 1.39 | - | 1.83 | 1.99 | 1.90 | 1.86 |
concentration(ng/μL) | 189.6 | - | 44.3 | 26.1 | 80.8 | 121.5 |
Number | YeWGAP5 | YeWGAP6 | ADU1 | ADU2 | BDW3 | BDW4 |
---|---|---|---|---|---|---|
A260/A280 | 1.81 | 1.70 | 1.89 | 1.65 | 1.82 | 1.87 |
A260/A230 | 0.63 | 0.75 | 2.21 | 0.79 | 1.45 | 2.10 |
concentration(ng/μL) | 41.3 | 14.4 | 44.8 | 124.5 | 39.8 | 46.7 |
Recorder: Chenyang Li and Weijie Jin Transformation of plasmid YeWGAP
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