Line 154: | Line 154: | ||
<div class="menu"> | <div class="menu"> | ||
<a class="item" href="https://2016.igem.org/Team:USTC/Team"> Members </a> | <a class="item" href="https://2016.igem.org/Team:USTC/Team"> Members </a> | ||
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Sponsor"> Sponsor </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/AboutUSTC"> About USTC </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Acknowledgements"> Acknowledgements </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Gallery"> Gallery </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Collaborations"> Collaborations </a> | |
</div> | </div> | ||
</div> | </div> | ||
Line 219: | Line 219: | ||
<div class="menu"> | <div class="menu"> | ||
<a class="item" href="https://2016.igem.org/Team:USTC/Team"> Members </a> | <a class="item" href="https://2016.igem.org/Team:USTC/Team"> Members </a> | ||
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Sponsor"> Sponsor </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/AboutUSTC"> About USTC </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Acknowledgements"> Acknowledgements </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Team/Gallery"> Gallery </a> | |
− | + | <a class="item" href="https://2016.igem.org/Team:USTC/Collaborations"> Collaborations </a> | |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 18:21, 19 October 2016
Notebook
Together we stand
Performers
Plasmid Extraction for sg11 Recorder: Dong Yan & Kaiyue Ma
Sample:bacteria containing recombinant plasmid pYeT containing sg11
Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.89 | 1.86 | 1.76 | 1.88 | 1.87 | 1.81 | 1.86 | 1.88 | |
A260/230 | 2.27 | 2.02 | 1.23 | 2.12 | 2.23 | 1.45 | 1.82 | 2.25 | |
concentration(ng/μL) | 159.3 | 113.5 | 265.7 | 46.8 | 130.5 | 189.3 | 59.7 | 169.3 |
sample | AD | BD | YeUGAP | ||||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.89 | 1.84 | 1.86 | ||||||
A260/230 | 0.48 | 0.85 | 0.79 | ||||||
concentration(ng/μL) | 13.1 | 25.1 | 33.9 |
sample | 08171001 | 08171002 | 08171003 | 08171004 | 08171005 | 08171006 | |||
---|---|---|---|---|---|---|---|---|---|
A260/280 | 1.89 | 1.83 | 1.80 | 1.70 | 2.88 | 1.89 | |||
A260/230 | 2.36 | 1.83 | 1.47 | 0.99 | 2.07 | 2.14 | |||
concentration(ng/μL) | 916.3 | 359.3 | 300.5 | 193.6 | 53.6 | 132.2 |
Sample | AD(0230) | BD(0231) | PYU(0232) | ||||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.97 | 1.83 | 1.89 | ||||||
A260/A280 | 0.12 | 0.41 | 0.75 | ||||||
concentration(ng/μL) | 24.8 | 62.5 | 105.4 |
Number | 1 | 2 | 3 | 4 | |||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.93 | 1.89 | 1.92 | 1.93 | |||||
A260/230 | 2.04 | 1.46 | 1.38 | 2.04 | |||||
concentration(ng/μL) | 43.2 | 19.7 | 49.3 | 20.8 |
number | YeWGAP18171001 | YeWGAP18171002 | YeWGAP18171003 | YeWGAP18171004 | ADU1001 | ADU1002 | BDW1003 | BDW1004 | |
---|---|---|---|---|---|---|---|---|---|
sample | YeWGAP08171001 | YeWGAP08171002 | YeWGAP08171003 | YeWGAP08171004 | ADU1 | ADU2 | BDW3 | BDW4 | |
nuclease-free water(μL) | 8.4 | 76 | 7.3 | 5.4 | 0 | 4 | 0 | 0 | |
10*Green Buffer(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
plasmid(μL) | 0.6 | 1.4 | 1.7 | 2.6 | 8 | 4 | 8 | 8 | |
EcoRI(μL) | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | 0.75 | |
PstI(μL) | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
number | YeWGAPD8181001 | YeWGAPD8181006 | BDD8181001 | BDD8181002 | |||||
---|---|---|---|---|---|---|---|---|---|
sample | YeWGAP08171001 | YeWGAP08171006 | BD(336) | BD(336) | |||||
nuclease-free water(μL) | 15.6 | 9 | 13.6 | 13.6 | |||||
10* Fast Digest Buffer(μL) | 2 | 2 | 2 | 2 | |||||
plasmid(μL) | 1 | 7.6 | 3 | 3 | |||||
EcoRI(μL) | 1 | 1 | 1 | 1 | |||||
PstI(μL) | 0.4 | 0.4 | 0.4 | 0.4 |
Number | 1 | 2 | 3 | 4 | |||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.84 | 1.87 | 1.91 | 1.87 | |||||
A260/230 | 2.30 | 2.34 | 2.40 | 2.28 | |||||
concentration(ng/μL) | 634.4 | 459.6 | 806.8 | 387.5 |
sample | YeWGAPD8181001 | BDD8181001 | |||||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.68 | 1.84 | |||||||
A260/A230 | 0.2 | 0.15 | |||||||
concentration(ng/μL) | 19.8 | 21.4 | |||||||
volume(μL) | 50 | 25 |
sample | AD | BD | sup35 | ||||||
---|---|---|---|---|---|---|---|---|---|
nuclease-free water(μL) | 22 | 22 | 32.5 | ||||||
2×HiFi-PCR Master(μL) | 25 | 25 | 0 | ||||||
template(μL) | 1 | 1 | 1 | ||||||
5×PrimeStar Buffer(μL) | 0 | 0 | 10 | ||||||
dNTP(μL) | 0 | 0 | 4 | ||||||
PrimeStar | 0 | 0 | 0.5 | ||||||
Biobrick Primer-r | 2 | 2 | 1 | ||||||
Biobrick Primer-p | 2 | 2 | 1 |
Number | AD1 | AD2 | BD1 | BD2 | sup35-1 | sup35-2 | sup35-3 | sup35-4 | |
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.83 | 1.83 | 1.85 | 1.86 | 1.80 | 1.72 | 1.84 | 1.78 | |
A260/A230 | 1.83 | 2.20 | 2.10 | 2.18 | 1.05 | 0.85 | 1.08 | 1.27 | |
concentration(ng/μL) | 212.3 | 200.6 | 186.0 | 230.9 | 12.3 | 21.2 | 21.5 | 20.9 |
Number | YeWGAP08181007 | YeWGAP08181008 | YeWGAP08181009 | YeWGAP08181010 | |||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.81 | 1.86 | 1.86 | 1.85 | |||||
concentration(ng/μL) | 740.5 | 841.2 | 725.0 | 695.9 |
number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | ||
---|---|---|---|---|---|---|---|---|---|
sample | YeWGAP08171001 | YeWGAP08171001 | YeWGAP08171001 | YeWGAP08171001 | BD(336) | BD(336) | BD(230.9) | ||
nuclease-free water(μL) | 11 | 11 | 11 | 11 | 12 | 14.5 | 6.4 | ||
10* Fast Digest Buffer(μL) | 2 | 2 | 2 | 2 | 2 | 2 | 2 | ||
plasmid(μL) | 5 | 5 | 5 | 5 | 5 | 3 | 10 | ||
EcoRI(μL) | 1.5 | 1.5 | 1.5 | 1.5 | 0.7 | 0.3 | 1.2 | ||
PstI(μL) | 0.5 | 0.5 | 0.5 | 0.5 | 0.3 | 0.2 | 0.4 |
Number | YeWGAP08181011 | YeWGAP08181012 | YeWGAP08181013 | YeWGAP08181014 | |||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.89 | 1.87 | 1.88 | 1.89 | |||||
concentration(ng/μL) | 818.6 | 416.9 | 807.4 | 900.6 |
Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
---|---|---|---|---|---|---|---|---|---|
concentration(ng/μL) | 128.1 | 228.7 | 60.5 | 67.0 | 398.3 | 291.6 | 80.8 | 101.1 | |
A260/A280 | 1.83 | 1.73 | 1.93 | 1.93 | 1.86 | 1.82 | 1.90 | 1.86 | |
A260/230 | 2.00 | 1.12 | 2.75 | 2.69 | 2.39 | 1.84 | 2.48 | 1.99 |
sample | YeWGAPD8191002 | BDD8191002 | |||||||
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.86 | 1.93 | |||||||
A260/A230 | 0.72 | 0.14 | |||||||
concentration(ng/μL) | 315.1 | 52.5 |
Number | -2-1 | -2-2 | -2-3 | -4-1 | -4-2 | -4-3 | -5-1 | -5-2 | -5-3 |
---|---|---|---|---|---|---|---|---|---|
A260/A280 | 1.89 | 1.90 | 1.86 | 1.83 | 1.81 | 1.92 | 1.89 | 1.93 | 1.82 |
A260/230 | 2.29 | 2.19 | 1.56 | 1.56 | 1.88 | 2.18 | 1.71 | 2.20 | 1.33 |
concentration(ng/μL) | 77.3 | 72.4 | 61.7 | 120.5 | 77.4 | 57.6 | 81.7 | 79.7 | 68.4 |
sample | PSB1C3:0802 |
---|---|
A260/A280 | 1.86 |
A260/A230 | 1.28 |
concentration(ng/μL) | 119.3 |
Ligase of PSB1C3 and BD Recorder: Yinchenguang Lyu
1μL PSB1C3 4μL BD 2μL 10×T4 DNA Ligase Buffer 0.4μL T4 DNA Ligase 12.6μL ddH2O
Under CC License
Based on Semantic-UI