Line 658: | Line 658: | ||
</div> | </div> | ||
+ | <div style="background-color:#44a17d; color:rgba(255, 255, 255, 0.9);"> | ||
+ | <div class="ui grid"> | ||
+ | |||
+ | <div class="ui three wide column"> | ||
+ | </div> | ||
+ | <div class="ui five wide column"> | ||
+ | <h3 class="ui header" style="color:rgba(255, 255, 255, 0.9);">Contact us</h3> | ||
+ | <div class="ui massive link list" style="color:rgba(255, 255, 255, 0.9);"> | ||
+ | <a class="item" href="mailto:yukae@mail.ustc.edu.cn"> | ||
+ | <i class="mail icon" style="color:rgba(255, 255, 255, 0.9);"></i> | ||
+ | <div class="content" style="color:rgba(255, 255, 255, 0.9);">e-mail</div> | ||
+ | </a> | ||
+ | <a class="item" href="https://www.facebook.com/ustcigem"> | ||
+ | <i class="facebook icon" style="color:rgba(255, 255, 255, 0.9);"></i> | ||
+ | <div class="content" style="color:rgba(255, 255, 255, 0.9);">Facebook</div> | ||
+ | </a> | ||
+ | <a class="item" href="http://weibo.com/3929719655/fans?topnav=1&wvr=6&mod=message&need_filter=1"> | ||
+ | <i class="weibo icon" style="color:rgba(255, 255, 255, 0.9);"></i> | ||
+ | <div class="content" style="color:rgba(255, 255, 255, 0.9);">Weibo</div> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="ui two wide column"> | ||
+ | </div> | ||
+ | <div class="ui five wide column"> | ||
+ | <h3 class="ui header" style="color:rgba(255, 255, 255, 0.9);">Sponsors</h3> | ||
+ | <img class="ui small image" src="https://static.igem.org/mediawiki/2016/c/cc/T--USTC--DeutscheBank.png"> | ||
+ | <img class="ui small image" src="https://static.igem.org/mediawiki/2016/1/18/T--USTC--USTCXiaohui.png"> | ||
+ | <img class="ui small image" src="https://static.igem.org/mediawiki/2016/4/45/T--USTC--USTCJiaowuchu.png"> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <div class="ui divider"></div> | ||
+ | <div class="center aligned container"> | ||
+ | <img class="ui centered tiny image" src="https://static.igem.org/mediawiki/2016/6/6b/T--USTC--MascotLogo.png"> | ||
+ | <div class="ui list"> | ||
+ | <div class="item">Designed by 2016 iGEM Team:USTC<br>Under CC License<br>Based on Semantic-UI</div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
</div><!--end of pusher--> | </div><!--end of pusher--> | ||
<script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script> | <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script> |
Revision as of 18:23, 19 October 2016
Notebook
Together we stand
Performers
DATE:8.20
Ligation of YeWGAP and BD **Recorder: Chenyang Li **
Procedure: 9 μL YeWGAPD8201002 3.3 μL BDD8201002 0.2 μL T4 DNA ligase 2 μL 10×T4 DNA Ligase Buffer 5.5 μL ddH2O 20 μL in total.
Mix gently and incubate at 22 degree Celsius for an hour.
The number of the product: YBL8201002 and YBL8201003
Then we do transformation.
**Recorder: Chenyang Li ** PCR of Sup35
Procedure: 9 μL YeWGAPD8201002 3.3 μL BDD8201002 0.2 μL T4 DNA ligase 2 μL 10×T4 DNA Ligase Buffer 5.5 μL ddH2O 20 μL in total.
Procedure:
1.Prepare 4 PCR tubes and sequentially add:
sample | sup35 | |||
---|---|---|---|---|
nuclease-free water(μL) | 30.5 | |||
5×PrimeStar Buffer(μL) | 10 | |||
dNTP(μL) | 4 | |||
template(μL) | 4 | |||
PrimeStar | 0.5 | |||
Primer1 | 0.5 | |||
Primer2 | 0.5 |
stage | temperature | time | ||
---|---|---|---|---|
Pre-Duration | 95 | 7 min | ||
Duration | 95 | 10s | ||
Anneal | 57 | 15s | ||
Extend | 72 | 50s | ||
Post-Extend | 72 | 10 min | ||
Final | 4 | -- |
Number | Sup35P8201001 | Sup35P8201002 | Sup35P8201003 | Sup35P8201004 |
---|---|---|---|---|
A260/A280 | 1.87 | 1.88 | 1.86 | 1.87 |
A260/A230 | 2.29 | 2.29 | 1.91 | 2.13 |
concentration(ng/μL) | 170.2 | 302.3 | 334.6 | 301.0 |
number | 1 | 2 | 3 | 4 |
---|---|---|---|---|
sample | Sup35P8201002 | Sup35P8201002 | Sup35P8201004 | Sup35P8201004 |
nuclease-free water(μL) | 4 | 7.8 | 4 | 7.8 |
10* Fast Digest Buffer(μL) | 2 | 2 | 2 | 2 |
plasmid(μL) | 10 | 7 | 10 | 7 |
EcoRI(μL) | 3 | 2.4 | 3 | 2.4 |
Bcul(μL) | 2.4 | 0.8 | 2.4 | 0.8 |
stage | temperature | time | ||
---|---|---|---|---|
step 1 | 95 | 5 min | ||
step 2 | 95 | 50 s | ||
step 3 | 56 | 1 min | ||
step 4 | 72 | 1 min 50 s | ||
step 5 | 72 | 10 min | ||
step 6 | 4 | -- |
sample | 1 | 2 | 3 | 4 |
---|---|---|---|---|
A260/A280 | 1.81 | 1.77 | 1.70 | 1.57 |
A260/A230 | 1.64 | 1.43 | 0.79 | 0.78 |
concentration(ng/μL) | 175.9 | 204.4 | 216.3 | 346.1 |
sample | 1,2,3,4,5,6,7,8 | |
---|---|---|
Sterilized ddH2O | 9 μL | |
2×HiFi-PCR Master | 12.5 μL | |
Template | 1.5 μL | |
Primer 1 | 1 μL | |
Primer 2 | 1 μL | |
total | 25 μL |
stage | temperature | time |
---|---|---|
Pre-Duration | 95℃ | 10 min |
Duration | 95℃ | 1 min |
Anneal | 58℃ | 1 min |
Extend | 72℃ | 1 min 50 s |
Post-Extend | 72℃ | 10 min |
Final | 4℃ | -- |
30 cycles(Duration ~ Extend)
Recorder: Yu Xie Double digestion of sfGFP11
Recorder: Yu Xie Double digestion of sup35
Under CC License
Based on Semantic-UI