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− | <h3> Team | + | <h3> Team Fungi </h3> |
<h4> Overview </h4> | <h4> Overview </h4> | ||
− | <p> | + | <p>This research aimed to (1) design a small library of level 0 parts for filamentous fungi, which consisted in two promoters, one fluorescent reporter, one terminator, an antibiotic resistance cassette and the Autonomously maintained in Aspergillus sequence, all of them in the Phytobrick standard from iGEM, (3) improve the iLOV fluorescent protein by adding the phytobrick standard (3) assemble expression cassettes with the mentioned parts, (5) transform P. roqueforti and (5) characterise the expression of the designed constructs . </p> |
<h4> Key Achievements </h4> | <h4> Key Achievements </h4> | ||
− | <p>- | + | <p>- Construction and submission of LV0 parts for filamentous fungi: PgdaA constitutive promoter (BBa_K1968009), PglaA inducible promoter (BBa_K1968013), Tcyc terminator (BBa_K1968014), Phleomycin antibiotic resistance cassette (BBa_K1968015) Improved iLOV in phytobrick standard (BBa_K1968016)</p> |
− | + | ||
− | <p>- | + | <p>- Design combinatorial gblocks for the AMA1 sequence to be assembled by Gibson</p> |
− | + | ||
<h4> Unachieved Results </h4> | <h4> Unachieved Results </h4> | ||
− | <p>- | + | <p>- Unable to experimentally characterize the PglaA (BBa_K1968009) and PglaA (BBa_K1968013) promoters</p> |
− | <p>- | + | <p>- Unsuccessful gibson assembly for the AMA1 sequence</p> |
+ | <p>- Transformation of P. roqueforti</p> | ||
</div> | </div> | ||
<div class="col-sm-2"></div> | <div class="col-sm-2"></div> |
Revision as of 19:19, 19 October 2016
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