Difference between revisions of "Team:Bulgaria/Safety"

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{{Bulgaria}}
 
{{Bulgaria}}
 
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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        }
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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        }
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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        .intro{
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            padding: 60px;
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</head>
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<body>
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<div class="wrapper">
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    <nav>
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        <ul class="navigation-bar">
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            <li class="menu-item">
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                <a href="https://2016.igem.org/Team:Bulgaria">home</a>
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            </li>
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            <li class="menu-item drop-link"> project
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                <ul class="project-submenu dropdown-content">
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Overview">overview</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Results">results</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/FutureWork">future work</a></li>
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                </ul>
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            </li>
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            <li class="menu-item drop-link">notebook
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                <ul class="notebook-submenu dropdown-content">
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/LabBook">lab book</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Organisms">organisms</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Safety">safety</a></li>
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                </ul>
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            <li class="menu-item"><a href="lab.html">lab</a></li>
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            <li class="menu-item drop-link">achievements
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                <ul class="achievements-submenu dropdown-content">
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/MedalCriteria">medal criteria</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Part">parts</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/HelpGuide">help guide</a></li>
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                </ul>
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            </li>
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            <li class="menu-item drop-link"><a href="https://2016.igem.org/Team:Bulgaria/HP/Silver">human practices</a>
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                <ul class="humanpractice-submenu dropdown-content">
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Engagement">public engagement</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Integrated_Practices">integrated human practices</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Collaborations">collaboration</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Entrepreneurship">enterpreneurship</a></li>
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                </ul>
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            </li>
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            <li class="menu-item drop-link">team
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                <ul class="team-submenu dropdown-content">
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Members">members</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/OurStory">our story</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Attributions">attributions</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/University">university</a></li>
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                    <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Bulgaria">Bulgaria</a></li>
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                </ul>
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        </ul>
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    <main>
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        <p class="intro">
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            Our project requires working environment corresponding to
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            Biosafety level 1. The microorganisms that we used to
 +
            construct our parts and express the proteins of interest
 +
            are different non-pathogenic strains of E. coli which do
 +
            not pose health risks. These strains are:
 +
        </p>
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        <ul class="strains">
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            <li class="strain">
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                Top10
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            </li>
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            <li class="strain">
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                JW1
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            </li>
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            <li class="strain">
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                Nova Blue
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            </li>
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            <li class="strain">
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                DH5α
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            </li>
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            <li class="strain">
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                Rosetta gami
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            </li>
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        </ul>
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        <p class="intro">
 +
            To protect ourselves from various risk factors in the laboratory
 +
            (and to protect our experiments from contamination as well) we
 +
            have created a set of rules that were strictly followed by all
 +
            team members. Those rules included working with standard protective
 +
            equipment – lab coats, gloves and safety googles. Wearing contact
 +
            lenses, jewelry and open footwear was not allowed. Long hairs were
 +
            kept tied. Machines like the autoclave that may pose risk were
 +
            properly maintained and handled with caution. Working with toxic
 +
            and volatile substances was conducted with face masks and in laminar
 +
            boxes in addition to the previously mentioned safety measures.
 +
            These and all other hazardous chemicals that may be dangerous for
 +
            the health or for the environment were disposed according to the
 +
            standard safety protocols. In addition general safety protocols for
 +
            lab behavior were applied accordingly. Each piece of equipment had
 +
            a responsible person that explained working protocols and safety
 +
            measure to all users.
 +
        </p>
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Revision as of 20:33, 19 October 2016

Our project requires working environment corresponding to Biosafety level 1. The microorganisms that we used to construct our parts and express the proteins of interest are different non-pathogenic strains of E. coli which do not pose health risks. These strains are:

  • Top10
  • JW1
  • Nova Blue
  • DH5α
  • Rosetta gami

To protect ourselves from various risk factors in the laboratory (and to protect our experiments from contamination as well) we have created a set of rules that were strictly followed by all team members. Those rules included working with standard protective equipment – lab coats, gloves and safety googles. Wearing contact lenses, jewelry and open footwear was not allowed. Long hairs were kept tied. Machines like the autoclave that may pose risk were properly maintained and handled with caution. Working with toxic and volatile substances was conducted with face masks and in laminar boxes in addition to the previously mentioned safety measures. These and all other hazardous chemicals that may be dangerous for the health or for the environment were disposed according to the standard safety protocols. In addition general safety protocols for lab behavior were applied accordingly. Each piece of equipment had a responsible person that explained working protocols and safety measure to all users.