Difference between revisions of "Team:SCUT-China A/Achievement part"

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             <p><span>T7 RNA polymerase induced by IPTG</span></p>
+
             <p><span>dszB with tac promoter induced by IPTG </span></p>
 
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             <p><span>coding</span></p>
+
             <p><span>Composite</span></p>
 
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             <p><span>T7 RNA polymerase will be expressed with IPTG added.</span></p>
+
             <p><span>dszB gene with tac promoter and terminator which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway.
 +
</span></p>
 
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             <p><span>4552 bp</span></p>
+
             <p><span>1307 bp</span></p>
 
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             <p><span>dszBCAD with T7 promoter induced by IPTG</span></p>
+
             <p><span>dszBBAC with tac promoter and dszD with lac promotor induced by IPTG </span></p>
 
           </div>
 
           </div>
 
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             <p><span>When IPTG added, dszBCAD genes with T7 promoter will be expressed greatly&nbsp; which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)</span></p>
+
             <p><span>dszBBAC with tac promoter and dszD with lac promotor induced by IPTG which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)</span></p>
 
           </div>
 
           </div>
 
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             <p><span>9097 bp</span></p>
+
             <p><span>7367 bp</span></p>
 
           </div>
 
           </div>
 
         </div>
 
         </div>

Revision as of 20:37, 19 October 2016

index

Type

Function

Length

dszA (a monooxygenase gene )

coding

convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway

1383 bp

dszB (a desulfinase gene

coding

convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway

1117 bp

dszC (a monooxygenase gene )

coding

not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway

1274 bp

dszD (a flavinreductase)

coding

three successive oxidation steps require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) during the 4S pathway

599 bp

dszA (a monooxygenase gene ), E.coli-optimized

coding

convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway

1383 bp

dszB (a desulfinase gene), E.coli-optimized

coding

convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway

1170 bp

dszC (a monooxygenase gene ), E.coli-optimized

coding

not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway

1274 bp

dszBCAD with T7 promoter

coding

dszBCAD genes with T7 promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

4537 bp

dszB with tac promoter induced by IPTG

Composite

dszB gene with tac promoter and terminator which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway.

1307 bp

dszBCAD with Lac promoter

coding

dszBCAD genes with Lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

5859 bp

dszBAC with tac promoter and dszD with lac promoter

coding

dszBAC genes with tac promoter and dszD gene with lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

6052 bp

dszBBAC with tac promoter and dszD with lac promotor induced by IPTG

composite

dszBBAC with tac promoter and dszD with lac promotor induced by IPTG which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

7367 bp

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