Difference between revisions of "Team:UGent Belgium/Primers"
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7624_Fw_Verification_pSB1C3</td> |
<td>CCACCTGACGTCTAAGAAAC</td> | <td>CCACCTGACGTCTAAGAAAC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7625_Rv_Verification_pSB1C3</td> |
<td>GTATTACCGCCTTTGAGTGA</td> | <td>GTATTACCGCCTTTGAGTGA</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7626_inaX?_fw</td> |
<td>ATGATCGTTGAAAAGATTTTGGCATTGCGTAC</td> | <td>ATGATCGTTGAAAAGATTTTGGCATTGCGTAC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7627_inaX?_rv</td> |
<td>TCAGACATCGCGCAGTTCCTCGCCAGTGCC</td> | <td>TCAGACATCGCGCAGTTCCTCGCCAGTGCC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7628_BsaI-inaZ_fw</td> |
<td>ACGTGGTCTCTGATGAATCTCGACAAGGCGTTG</td> | <td>ACGTGGTCTCTGATGAATCTCGACAAGGCGTTG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7629_BsaI-inaZ_rv*</td> |
<td>ACGTGGTCTCTAGTACTACTTTACCTCTATCCAGTC</td> | <td>ACGTGGTCTCTAGTACTACTTTACCTCTATCCAGTC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7643_Rv_BB_strong</td> |
<td>AGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG</td> | <td>AGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7644_Rv_BB_weak</td> |
<td>AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG</td> | <td>AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7645_Fw_BB</td> |
<td>TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAG</td> | <td>TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7658_inaZ_GGfix_2_Fw</td> |
<td>AGTAGGTCTCAGTAACGAAACCGCTGGCAACCACAGTGATC</td> | <td>AGTAGGTCTCAGTAACGAAACCGCTGGCAACCACAGTGATC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7659_inaZ_GGfix_3_Fw</td> |
<td>TAACGGTCTCAGGCGGGTGACCAAAGCAGATTGACCGCTG</td> | <td>TAACGGTCTCAGGCGGGTGACCAAAGCAGATTGACCGCTG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7661_GFP_Fw-ATCT</td> |
<td>AGTCGGTCTCAATCTAGTAAAGGAGAAGAACTTTTCACTGGAG</td> | <td>AGTCGGTCTCAATCTAGTAAAGGAGAAGAACTTTTCACTGGAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7662_GFP_Fw-GATG</td> |
<td>AGTCGGTCTCAGATGAGTAAAGGAGAAGAACTTTTCACTGGAG</td> | <td>AGTCGGTCTCAGATGAGTAAAGGAGAAGAACTTTTCACTGGAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7663_GFP_Rv-ATCT</td> |
<td>AGCTGGTCTCAAGATCCGGTGCTGCCTTTGTAGAGCTCATCCATGCCATG</td> | <td>AGCTGGTCTCAAGATCCGGTGCTGCCTTTGTAGAGCTCATCCATGCCATG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7664_GFP_Rv-TACT</td> |
<td>AGCTGGTCTCAAGTATTATTTGTAGAGCTCATCCATGCCATG</td> | <td>AGCTGGTCTCAAGTATTATTTGTAGAGCTCATCCATGCCATG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7665_INP_RC_Fw</td> |
<td>AGTCGGTCTCAGATGCCGCAGAATGTGCAGAC</td> | <td>AGTCGGTCTCAGATGCCGCAGAATGTGCAGAC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7666_INP_RC_Rv</td> |
<td>AGCTGGTCTCAAGATCCGGTGCTGCCCTTTACCTCTATCCAGTCATCGTCCTCGTC</td> | <td>AGCTGGTCTCAAGATCCGGTGCTGCCCTTTACCTCTATCCAGTCATCGTCCTCGTC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7667_Lpp_Fw</td> |
<td>AGTCGGTCTCAGATGAAAGCTACTAAACTGGTACTGG</td> | <td>AGTCGGTCTCAGATGAAAGCTACTAAACTGGTACTGG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7668_Strep_Rv</td> |
<td>AGTCGGTCTCAAGTATTATTAATGGTGGTGATGGTGATGGGA</td> | <td>AGTCGGTCTCAAGTATTATTAATGGTGGTGATGGTGATGGGA</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7669_FW_BB_LC</td> |
<td>TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGGAGTCACTAAGGGTTAG</td> | <td>TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGGAGTCACTAAGGGTTAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7670_RV_BB_weak_LC</td> |
<td>AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCGAGTCACTAAGGGCTAAC</td> | <td>AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCGAGTCACTAAGGGCTAAC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7671_RV_insert</td> |
<td>AGTATATAAACGCAGAAAGGCC</td> | <td>AGTATATAAACGCAGAAAGGCC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7672_FW_Insert_weak</td> |
<td>TTTACGGCTAGCTCAGTCCTAG</td> | <td>TTTACGGCTAGCTCAGTCCTAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7673_inaZ_GGfix_1_Rv</td> |
<td>CAATTAGATCACTGTGGTTGCCAG</td> | <td>CAATTAGATCACTGTGGTTGCCAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7674_inaZ_GGfix_2_Rv</td> |
<td>TCTTACCAGCGGTCAATCTGCTTTGGTC</td> | <td>TCTTACCAGCGGTCAATCTGCTTTGGTC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7675_FW_BB_pSB3K3</td> |
<td>TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGGCAAG</td> | <td>TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGGCAAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7676_BB_mSA2_Fw</td> |
<td>GCTGCAGTCCGGCAAAAAAGGGCAAG</td> | <td>GCTGCAGTCCGGCAAAAAAGGGCAAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7677_BB_mSA2_Rv</td> |
<td>CCGCGAATTCCAGAAATCATCCTTAGCG</td> | <td>CCGCGAATTCCAGAAATCATCCTTAGCG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7714_bb-pSC101_fw</td> |
<td>CCTAGGACTGAGCTAGCTGTCAATAGGGCAGCAGATAGGGACGAC</td> | <td>CCTAGGACTGAGCTAGCTGTCAATAGGGCAGCAGATAGGGACGAC</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7715_bb-pSC101_rv</td> |
<td>GTGGGCCTTTCTGCGTTTATATACTCTTTTATTGGAGAGGTGGACAAG</td> | <td>GTGGGCCTTTCTGCGTTTATATACTCTTTTATTGGAGAGGTGGACAAG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7722_BB_GFP_Fw</td> |
<td>ACGTGGTCTCATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTG</td> | <td>ACGTGGTCTCATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>oM7723_BB_GFP_Rv</td> |
<td>AGTCGGTCTCACATCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td> | <td>AGTCGGTCTCACATCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td> | ||
</tr> | </tr> | ||
Latest revision as of 21:30, 19 October 2016
Used primers
| Primer | Sequence |
|---|---|
| oM7624_Fw_Verification_pSB1C3 | CCACCTGACGTCTAAGAAAC |
| oM7625_Rv_Verification_pSB1C3 | GTATTACCGCCTTTGAGTGA |
| oM7626_inaX?_fw | ATGATCGTTGAAAAGATTTTGGCATTGCGTAC |
| oM7627_inaX?_rv | TCAGACATCGCGCAGTTCCTCGCCAGTGCC |
| oM7628_BsaI-inaZ_fw | ACGTGGTCTCTGATGAATCTCGACAAGGCGTTG |
| oM7629_BsaI-inaZ_rv* | ACGTGGTCTCTAGTACTACTTTACCTCTATCCAGTC |
| oM7643_Rv_BB_strong | AGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG |
| oM7644_Rv_BB_weak | AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG |
| oM7645_Fw_BB | TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAG |
| oM7658_inaZ_GGfix_2_Fw | AGTAGGTCTCAGTAACGAAACCGCTGGCAACCACAGTGATC |
| oM7659_inaZ_GGfix_3_Fw | TAACGGTCTCAGGCGGGTGACCAAAGCAGATTGACCGCTG |
| oM7661_GFP_Fw-ATCT | AGTCGGTCTCAATCTAGTAAAGGAGAAGAACTTTTCACTGGAG |
| oM7662_GFP_Fw-GATG | AGTCGGTCTCAGATGAGTAAAGGAGAAGAACTTTTCACTGGAG |
| oM7663_GFP_Rv-ATCT | AGCTGGTCTCAAGATCCGGTGCTGCCTTTGTAGAGCTCATCCATGCCATG |
| oM7664_GFP_Rv-TACT | AGCTGGTCTCAAGTATTATTTGTAGAGCTCATCCATGCCATG |
| oM7665_INP_RC_Fw | AGTCGGTCTCAGATGCCGCAGAATGTGCAGAC |
| oM7666_INP_RC_Rv | AGCTGGTCTCAAGATCCGGTGCTGCCCTTTACCTCTATCCAGTCATCGTCCTCGTC |
| oM7667_Lpp_Fw | AGTCGGTCTCAGATGAAAGCTACTAAACTGGTACTGG |
| oM7668_Strep_Rv | AGTCGGTCTCAAGTATTATTAATGGTGGTGATGGTGATGGGA |
| oM7669_FW_BB_LC | TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGGAGTCACTAAGGGTTAG |
| oM7670_RV_BB_weak_LC | AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCGAGTCACTAAGGGCTAAC |
| oM7671_RV_insert | AGTATATAAACGCAGAAAGGCC |
| oM7672_FW_Insert_weak | TTTACGGCTAGCTCAGTCCTAG |
| oM7673_inaZ_GGfix_1_Rv | CAATTAGATCACTGTGGTTGCCAG |
| oM7674_inaZ_GGfix_2_Rv | TCTTACCAGCGGTCAATCTGCTTTGGTC |
| oM7675_FW_BB_pSB3K3 | TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGGCAAG |
| oM7676_BB_mSA2_Fw | GCTGCAGTCCGGCAAAAAAGGGCAAG |
| oM7677_BB_mSA2_Rv | CCGCGAATTCCAGAAATCATCCTTAGCG |
| oM7714_bb-pSC101_fw | CCTAGGACTGAGCTAGCTGTCAATAGGGCAGCAGATAGGGACGAC |
| oM7715_bb-pSC101_rv | GTGGGCCTTTCTGCGTTTATATACTCTTTTATTGGAGAGGTGGACAAG |
| oM7722_BB_GFP_Fw | ACGTGGTCTCATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTG |
| oM7723_BB_GFP_Rv | AGTCGGTCTCACATCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
