Difference between revisions of "Team:Kent/Experiments"

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   <li>IPTG Stock (1M)
 
   <li>IPTG Stock (1M)
 
  </ul>
 
  </ul>
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<h2 class='sub_titles'>Nickel Ion Affinity Chromatography Column Buffers:</h2>
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<div class="picture">
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<img src="2016.igem.org/wiki/images/7/7e/TeamKentExpTable.png>
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</div>
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<h2 class='sub_titles'>Bacterial Transformation:</h2>
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Revision as of 21:57, 19 October 2016

Materials


Chemical Synthesis Materials:

  • Iron(III) Chloride Hexahydrate (0.4M)
  • Ethylene Glycol (Anhydrous)
  • Iron(III) Chloride (aq) Hexahydrate (1M)
  • Iron(II) Sulphate (HCl) Tetrahydrate (1M)
  • HCl (2M)
  • Ammonia Solution (0.7M)

Antibiotics for Plates:

  • Ampicillin 100 g/L stock
  • Chloramphenicol 25 g/L stock

Protein Induction Sugar:

  • IPTG Stock (1M)

Nickel Ion Affinity Chromatography Column Buffers: