Difference between revisions of "Team:Kent/Experiments"
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<h2 class='sub_titles'>Nickel Ion Affinity Chromatography Column Buffers:</h2> | <h2 class='sub_titles'>Nickel Ion Affinity Chromatography Column Buffers:</h2> | ||
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Revision as of 22:00, 19 October 2016
Materials
Chemical Synthesis Materials:
- Iron(III) Chloride Hexahydrate (0.4M)
- Ethylene Glycol (Anhydrous)
- Iron(III) Chloride (aq) Hexahydrate (1M)
- Iron(II) Sulphate (HCl) Tetrahydrate (1M)
- HCl (2M)
- Ammonia Solution (0.7M)
Antibiotics for Plates:
- Ampicillin 100 g/L stock
- Chloramphenicol 25 g/L stock
Protein Induction Sugar:
- IPTG Stock (1M)
Nickel Ion Affinity Chromatography Column Buffers:
Bacterial Transformation:
