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− | <figure> <img src="https://static.igem.org/mediawiki/2016/ | + | <figure> <img src="https://static.igem.org/mediawiki/2016/5/53/T--Tec-Monterrey--caro1.jpg" alt="" class="img-responsive"> |
<figcaption> | <figcaption> | ||
<p>Dedication, passion, responsible, organized, belly dancer, runner, coffee lover, baker.</p> | <p>Dedication, passion, responsible, organized, belly dancer, runner, coffee lover, baker.</p> | ||
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− | <figure> <img src="https://static.igem.org/mediawiki/2016/ | + | <figure> <img src="https://static.igem.org/mediawiki/2016/7/74/T--Tec-Monterrey--chuy1.jpg" alt="" class="img-responsive"> |
<figcaption> | <figcaption> | ||
<p>Careful planner, intelligent, realistic, honest, dramatic, semi-god like explaining skills, map lover.</p> | <p>Careful planner, intelligent, realistic, honest, dramatic, semi-god like explaining skills, map lover.</p> | ||
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− | <figure> <img src="https://static.igem.org/mediawiki/2016/ | + | <figure> <img src="https://static.igem.org/mediawiki/2016/1/1c/T--Tec-Monterrey--ale1.jpg" alt="" class="img-responsive"> |
<figcaption> | <figcaption> | ||
<p>Our Ale</p> | <p>Our Ale</p> | ||
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− | <figure> <img src="https://static.igem.org/mediawiki/2016/ | + | <figure> <img src="https://static.igem.org/mediawiki/2016/b/b6/T--Tec-Monterrey--carlos2.jpg" alt="" class="img-responsive"> |
<figcaption> | <figcaption> | ||
<p> Dreamer, independent, random, proactive, creative, funny, positive, crazy.</p> | <p> Dreamer, independent, random, proactive, creative, funny, positive, crazy.</p> | ||
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− | <figure> <img src="https://static.igem.org/mediawiki/2016/ | + | <figure> <img src="https://static.igem.org/mediawiki/2016/1/15/T--Tec-Monterrey--marcelo1.jpg" alt="" class="img-responsive"> |
<figcaption> | <figcaption> | ||
<p>Loyal, handsome, fit, social, loves partying, sensitive, gentleman, funny.</p> | <p>Loyal, handsome, fit, social, loves partying, sensitive, gentleman, funny.</p> | ||
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− | <figure> <img src="https://static.igem.org/mediawiki/2016/6/ | + | <figure> <img src="https://static.igem.org/mediawiki/2016/6/62/T--Tec-Monterrey--frutos1.jpg" alt="" class="img-responsive"> |
<figcaption> | <figcaption> | ||
<p>Smart, creative, sweet, fun, gamer, food giver, optimistic, IT God.</p><p>If it wasn’t for him, you wouldn’t be reading this. He is the computer science mind of the team, always giving advice and being there when the team needs a hug or food. Carlos was the one who created the wiki and helped the whole team to go through the tough days with words of wisdom. He sings High School Musical songs and dances better than all the girls together in the team. You will definitely love his sweet attitude towards life and his rage quits when the code doesn’t work. </p><p>Igem fact: He can make every code work in a matter of hours. </p><p>Random fact: He has a meme for everything and has the 721 Pokémon in existence registered in the Pokedex. </p> | <p>Smart, creative, sweet, fun, gamer, food giver, optimistic, IT God.</p><p>If it wasn’t for him, you wouldn’t be reading this. He is the computer science mind of the team, always giving advice and being there when the team needs a hug or food. Carlos was the one who created the wiki and helped the whole team to go through the tough days with words of wisdom. He sings High School Musical songs and dances better than all the girls together in the team. You will definitely love his sweet attitude towards life and his rage quits when the code doesn’t work. </p><p>Igem fact: He can make every code work in a matter of hours. </p><p>Random fact: He has a meme for everything and has the 721 Pokémon in existence registered in the Pokedex. </p> | ||
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− | <figure> <img src="https://static.igem.org/mediawiki/2016/ | + | <figure> <img src="https://static.igem.org/mediawiki/2016/6/67/T--Tec-Monterrey--naya1.jpg" alt="" class="img-responsive"> |
<figcaption> | <figcaption> | ||
<p>Engineering Physics student, smart, curious, kind.</p><p>Javier is our youngest member. He’s only in his third semester but has been going to courses ahead of his study plan. He is very dedicated and passionate about math and learning. In fact, he got into the team in a very curious way: he was invited to one of the “training” sessions and got instantly interested into Systems Biology and modeling. He is the analytical part of the modeling team and without him the model wouldn’t be as good as it is. He is always a very nice and kind person. He was somewhat shy at the beginning but not so much right now. One thing he keeps doing is not knocking on the lab door, instead he places his head against the glass and waits for someone to see him (the door is magnetically locked and it is made of glass but it’s not fully transparent).</p><p>iGEM fact: Every time he receives a voice note, he panics thinking something could have gone wrong with the math model.</p><p>Random fact: His last name is almost the same as his home state (Nayarit).</p> | <p>Engineering Physics student, smart, curious, kind.</p><p>Javier is our youngest member. He’s only in his third semester but has been going to courses ahead of his study plan. He is very dedicated and passionate about math and learning. In fact, he got into the team in a very curious way: he was invited to one of the “training” sessions and got instantly interested into Systems Biology and modeling. He is the analytical part of the modeling team and without him the model wouldn’t be as good as it is. He is always a very nice and kind person. He was somewhat shy at the beginning but not so much right now. One thing he keeps doing is not knocking on the lab door, instead he places his head against the glass and waits for someone to see him (the door is magnetically locked and it is made of glass but it’s not fully transparent).</p><p>iGEM fact: Every time he receives a voice note, he panics thinking something could have gone wrong with the math model.</p><p>Random fact: His last name is almost the same as his home state (Nayarit).</p> |
Revision as of 22:03, 19 October 2016
Meet the people who make awesome stuff
Carmen Padilla
Biotechnology engineer
Melissa González
Biotechnology engineer
Carolina Elizondo
Biotechnology engineer
Jesús García Lima
Biotechnology engineer
Alejandra Vela
Biotechnology Engineering
Yili Natalia Padilla
Biotechnology engineer
Carlos Vásquez
COCREADORADEIGEM
Raúl Garza
Creative Director
Melissa Rios
COCREADORADEIGEM
Marcelo Álvarez
Creative Director
Suria Itzel Morales
COCREADORADEIGEM
Carlos Frutos
Creative Director
Javier Naya
COCREADORADEIGEM
Javier Montemayor
Creative Director
Safe Lab Works
During the summer, we worked on the Laboratory of Molecular Diagnosis and Genetic Manipulation, which is at the installations of the FEMSA Biotechnology Center, located at our institution, Tecnológico de Monterrey, Campus Monterrey.
Since it is the first time Acidithiobacillus ferrooxidans and Chromobacterium violaceum are used on a laboratory inside the Center of Biotechnology, our advisors could only give us general guidance on their use. Two of our team members went to the Laboratory of Geomicrobiology of the Institute of Metallurgy of the Autonomous University of San Luis Potosí and were trained by Dr. Viridiana García Meza, so they could learn how to handle Acidithiobacillus ferrooxidans. The risk with this organism lies in the pH of its medium, which is extremely acid. Its handling must be used in an extraction hood and precautions such as using gloves must be taken.
C. violaceum is biosafety level 2 since it can act as an opportunistic pathogen to animals and humans, infecting only immunosuppressed people through skin lesions. Very few cases of this type of infections have been reported. Nevertheless, many of the protocols, such as cell culture and transformation are similar to those from Escherichia coli. Mentoring from Dr. Rodolfo García Contreras, from the Department of Microbiology and Parasitology from the Faculty of Medicine at UNAM was received through phone calls and emails for recommendations of its handling.
Our lab is biosafety level 2, so measurements such as controlled access and decontamination of work surfaces after completion of work. All the rules are established on the Biosafety in Microbiological and Biomedical Laboratories by the Centers for Disease Control and Prevention.
Safe Project Design
Even though one of the organisms we chose as our chassis is listed as biosafety level 2, our project does not present any danger to animals, humans or plants. But, one of the problems in module II, which is the bioleaching by C. violaceum, is the production of cyanide, which is required for the recovery of gold. For this, we designed a cyanide degradation system, where another C. violaceum strain will be modified in order to overexpress cyanide hydratase, a cyanide degradation enzyme.
Due to the complications in fully degrading the cyanide, we must determine the concentration of cyanide left at the end of the process in order to be able to do a proper disposal. This is done according to the NMX-AA-058-SCFI-2001 federal regulation, which establishes the guidelines for the determination of total cyanide in natural, drinking, wastewaters and wastewaters treated. Once this is done, the proper disposal of it must be done, according to the NOM-157-SEMARNAT-2009 federal norm, which establishes the elements and procedures to implement the disposal of mining residues, including cyanide.
References
Centers for disease control and prevention. (2009). Biosafety in Microbiological and Biomedical Laboratories. (5th Edition ed.). United States: HHS Publication.
Norma Oficial Mexicana NOM-157-SEMARNAT-2009. Diario Oficial de la Federación, 30 de agosto de 2011.
Norma Mexicana NMX-AA-058-SCFI-2001. Diario Oficial de la Federación, 09 de julio de 2009.