Difference between revisions of "Team:MIT/Genetic Circuit"
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| − | Above is a diagram of the larger genetic circuit we envision constructing with the individual parts we have characterized. The first component of the circuit (on the far left) checks for characteristics of the disease during the <b>estrogen-high</b> phase of the menstrual cycle and expresses the <b>first recombinase</b> if it senses them. The second component (in the middle) checks for characteristics of the disease during the <b>progesterone-high</b> phase of the cycle and expresses the <b>second recombinase</b> if it senses them. The final component contains the fluorescent output of the circuit - in healthy cells, the transcriptional stop signal between the promoter and the gene, as well as the gene’s being inverted, suppress the expression of yellow fluorescence. In cells with the disease, however, the first recombinase is expressed and cuts out the terminator sequence, and the second recombinase is expressed and inverts the gene to the correct orientation, allowing expression of eYFP. This biological ‘and’ gate with two temporally distinct checks for the disease greatly increases the specificity for an accurate diagnosis. | + | Above is a diagram of the larger genetic circuit we envision constructing with the individual parts we have characterized. The first component of the circuit (on the far left) checks for characteristics of the disease during the <b>estrogen-high</b> phase of the menstrual cycle and expresses the <b>first recombinase</b> if it senses them. The second component (in the middle) checks for characteristics of the disease during the <b>progesterone-high</b> phase of the cycle and expresses the <b>second recombinase</b> if it senses them. The final component contains the fluorescent output of the circuit - in healthy cells, the transcriptional stop signal between the promoter and the gene, as well as the gene’s being inverted, suppress the expression of yellow fluorescence. In cells with the disease, however, the first recombinase is expressed and cuts out the terminator sequence, and the second recombinase is expressed and inverts the gene to the correct orientation, allowing expression of eYFP. This biological ‘and’ gate with two temporally distinct checks for the disease greatly increases the specificity for an accurate diagnosis. The following walk-through will detail the mechanics of how the circuit works. |
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Revision as of 22:13, 19 October 2016
Genetic Circuit Design
Above is a diagram of the larger genetic circuit we envision constructing with the individual parts we have characterized. The first component of the circuit (on the far left) checks for characteristics of the disease during the estrogen-high phase of the menstrual cycle and expresses the first recombinase if it senses them. The second component (in the middle) checks for characteristics of the disease during the progesterone-high phase of the cycle and expresses the second recombinase if it senses them. The final component contains the fluorescent output of the circuit - in healthy cells, the transcriptional stop signal between the promoter and the gene, as well as the gene’s being inverted, suppress the expression of yellow fluorescence. In cells with the disease, however, the first recombinase is expressed and cuts out the terminator sequence, and the second recombinase is expressed and inverts the gene to the correct orientation, allowing expression of eYFP. This biological ‘and’ gate with two temporally distinct checks for the disease greatly increases the specificity for an accurate diagnosis. The following walk-through will detail the mechanics of how the circuit works.
Healthy cells +Estradiol
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo.
Healthy cells +Progesterone
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo.Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo.
Disease cells +Estradiol
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo.Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo.
Disease cells +Progesterone
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo.Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean laoreet eu ligula facilisis euismod. Curabitur mattis ligula eget enim maximus, dignissim congue ex lacinia. Maecenas pharetra libero ut hendrerit venenatis. Sed eget enim purus. Nam nec sapien felis. In hac habitasse platea dictumst. Etiam laoreet sem ut quam sollicitudin, nec feugiat elit tempor. Donec interdum, felis eget mollis hendrerit, enim eros auctor mauris, ac feugiat nibh libero nec nunc. Aenean libero quam, luctus quis sodales sed, condimentum vel augue. Ut et nibh eu urna faucibus consequat. Pellentesque malesuada egestas augue vitae molestie. Aliquam sed ullamcorper dui. Nunc nec ante justo.
