Difference between revisions of "Team:Nagahama/Description"

 
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{{Nagahama}}
 
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__NOTOC__
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It is a sequel to our theme of the last year.
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<partinfo>BBa_K1230000 short</partinfo>
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Here, we explain our theme of the last year.
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In last year, we succeeded in
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In this year, we had 3 purposes in our study.
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The MarA protein acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.
  
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'''Project description'''
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We used device BBa_K1230004 to characterise this part.
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Team iGEM Nagahama 2015
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To innovate a refrigerator without electricity, we invented a prototype of FRAVORATOR that is possible to preserve food by antibacterial volatiles synthesized<br /> by Escherichia coli.
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We improved the characterization of a previously existing BioBrick Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' gene as [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.
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For high-efficiency synthetic processs, we tried two methods. 1, 1-deoxy-D-xylulose 5-phosphate（DXP）synthase, Dxs, is down-regulated when its downstream<br /> material has been produced to some extent. So, we tried to over-express, another DXP synthase, nDXP, that rarely has been used in normal conditions. 2, Using genomic editing method, we tried to knock out synthase, that we used to produce unnecessary by product.
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Team Nagahama 2016
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We improved the characterization of existing this Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000]. We found that marA expression is also improve resistance to farnesol. Result page is [https://2016.igem.org/Team:Nagahama/Result_and_Discussion here]
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1230000 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K1230000 parameters</partinfo>
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Latest revision as of 22:50, 19 October 2016


<partinfo>BBa_K1230000 short</partinfo>

The MarA protein acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.

We used device BBa_K1230004 to characterise this part.

Team iGEM Nagahama 2015
We improved the characterization of a previously existing BioBrick Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick marA gene as [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.


Team Nagahama 2016
We improved the characterization of existing this Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000]. We found that marA expression is also improve resistance to farnesol. Result page is here


Sequence and Features <partinfo>BBa_K1230000 SequenceAndFeatures</partinfo>