Line 47: | Line 47: | ||
<div class="col-sm-5"> | <div class="col-sm-5"> | ||
<img src="https://static.igem.org/mediawiki/2016/3/34/T--Edinburgh_OG--Matin_CARE_criteria.png" width="100%"> | <img src="https://static.igem.org/mediawiki/2016/3/34/T--Edinburgh_OG--Matin_CARE_criteria.png" width="100%"> | ||
+ | <br><br><a href="https://static.igem.org/mediawiki/2016/2/29/T--Edinburgh_OG--Anette_CARE_CODE.zip" class="page-scroll btn btn-default btn-xl sr-button">Download List</a> | ||
+ | |||
</div> | </div> | ||
<div class="col-sm-7" | <div class="col-sm-7" | ||
Line 52: | Line 54: | ||
-moz-column-count: 2; /* Firefox */ | -moz-column-count: 2; /* Firefox */ | ||
column-count: 2;"> | column-count: 2;"> | ||
− | <p> According to the toxic effect of these compounds on human health, animal health and the environment, a quantitative criterion for clear toxicity scores was created using a scale from 1 to 5 and a “traffic light” colour scheme. If the reviewed information showed that the secondary metabolite did not affect in a negative manner any of the three parameters, it was considered non-toxic and categorised as a “1” (green). If the data showed that the compound exhibited some degree of toxicity but nothing considerably serious or untreatable, it was considered slightly to moderately toxic and scored as a “3” (yellow). And if it showed that the secondary metabolite was extremely toxic, it was considered as highly toxic and classified as a “5” (red). However, considering there were three parameters to be contemplated, combinations of those three scores could occur (e.g., a secondary metabolite being considered non-toxic for humans but slightly to moderately toxic to animals). Therefore, if a compound was non-toxic according to one parameter and slightly to moderately toxic for the others, it was categorised as a “2” (green-yellow). On the other hand, if it was slightly to moderately toxic according to some parameter and highly toxic for others, it was classified as a “4” (yellow-red). Finally, if the secondary metabolite had no documented toxicity information, or if there was no specific BGC and just the general class of secondary metabolite or microorganism, the compound was categorised as “?” (black) with the note to consider if, according to the precautionary principle , one should prusue with the use of the given microorganism in the laboratory. | + | <p> According to the toxic effect of these compounds on human health, animal health and the environment, a quantitative criterion for clear toxicity scores was created using a scale from 1 to 5 and a “traffic light” colour scheme. If the reviewed information showed that the secondary metabolite did not affect in a negative manner any of the three parameters, it was considered non-toxic and categorised as a “1” (green). If the data showed that the compound exhibited some degree of toxicity but nothing considerably serious or untreatable, it was considered slightly to moderately toxic and scored as a “3” (yellow). And if it showed that the secondary metabolite was extremely toxic, it was considered as highly toxic and classified as a “5” (red). However, considering there were three parameters to be contemplated, combinations of those three scores could occur (e.g., a secondary metabolite being considered non-toxic for humans but slightly to moderately toxic to animals). Therefore, if a compound was non-toxic according to one parameter and slightly to moderately toxic for the others, it was categorised as a “2” (green-yellow). On the other hand, if it was slightly to moderately toxic according to some parameter and highly toxic for others, it was classified as a “4” (yellow-red). Finally, if the secondary metabolite had no documented toxicity information, or if there was no specific BGC and just the general class of secondary metabolite or microorganism, the compound was categorised as “?” (black) with the note to consider if, according to the precautionary principle , one should prusue with the use of the given microorganism in the laboratory. |
+ | <br><br>As a proof of concept, the 30 organisms were screened using the CARE tool and the results showed that, although some secondary metabolites were considered as toxic (e.g. roquefortine, PR-toxin, isofumigaclavine and mycophenolic acid for P. roqueforti), their amounts do not affect human health, meaning that these organisms were safe to work with in laboratory settings and we could proceed with the experimentation with them. | ||
+ | <br><br>When a laboratory works on a non-model organism with the objective of harnessing its industrial potential, genome editing processes may be involved (e.g., CRISPR-Cas9 tool). Therefore, this tool could additionally be used in order to determine differences in toxicity between native and genetically modified organisms. | ||
</p> | </p> | ||
</div> | </div> |
Revision as of 23:40, 19 October 2016
-->