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<p><center><font color="silver"><h2>Project Design</h2></font></center></p> | <p><center><font color="silver"><h2>Project Design</h2></font></center></p> | ||
<p><center><font color="silver">In order to combat the nitrate problem, we aimed to design a strain of <i>E. coli</i> capable of reducing these excess nitrate ions. By isolating a gene that codes of periplasmic nitrate reductases, we began the process of designing our synthetic organism.</font></center></p> | <p><center><font color="silver">In order to combat the nitrate problem, we aimed to design a strain of <i>E. coli</i> capable of reducing these excess nitrate ions. By isolating a gene that codes of periplasmic nitrate reductases, we began the process of designing our synthetic organism.</font></center></p> | ||
+ | <p><center><font color="silver">To read more information about the design of our system, click the image below.</font></center></p> | ||
+ | <center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/design"><img src="https://static.igem.org/mediawiki/2016/0/0f/T--UNebraska-Lincoln--design.png" style="width:40%; height:auto;" alt="image"/></a></center> | ||
+ | <p><center><font color="silver"><h2>Experiments for Our System</h2></font></center></p> | ||
+ | <p><center><font color="silver">In order to combat the nitrate problem, we aimed to design a strain of <i>E. coli</i> capable of reducing these excess nitrate ions. By isolating a gene that codes of periplasmic nitrate reductases, we began the process of designing our synthetic organism.</font></center></p> | ||
+ | <p><center><font color="silver">To read more information about the design of our system, click the image below.</font></center></p> | ||
+ | <center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/design"><img src="https://static.igem.org/mediawiki/2016/0/0f/T--UNebraska-Lincoln--design.png" style="width:40%; height:auto;" alt="image"/></a></center> | ||
+ | <p><center><font color="silver"><h2>Recharacterization of K381001</h2></font></center></p> | ||
+ | <p><center><font color="silver">In the design of our project, we implemented a nitrate-sensitive kill switch. This took advantage of the fact that the yeaR promoter, originally submitted by the Edinburgh iGEM Team in 2009 (<a href="http://parts.igem.org/Part:BBa_K216005">K216005</a>. We decided to begin our project by determining sensitivity of this promoter the concentrations of nitrate that would be present in waterways. To do this, we recharacterized the composite part <a href="http://parts.igem.org/Part:BBa_K381001">K381001</a>, a PyeaR-GFP nitrate sensor. You can read more about our rechracterization of the PyeaR-GFP Biobrick <a href="http://parts.igem.org/Part:BBa_K381001:Experience#UNebraska-Lincoln_2016>Here</a>.</font></center></p> | ||
<p><center><font color="silver">To read more information about the design of our system, click the image below.</font></center></p> | <p><center><font color="silver">To read more information about the design of our system, click the image below.</font></center></p> | ||
<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/design"><img src="https://static.igem.org/mediawiki/2016/0/0f/T--UNebraska-Lincoln--design.png" style="width:40%; height:auto;" alt="image"/></a></center> | <center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/design"><img src="https://static.igem.org/mediawiki/2016/0/0f/T--UNebraska-Lincoln--design.png" style="width:40%; height:auto;" alt="image"/></a></center> | ||
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Revision as of 00:31, 20 October 2016
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