Difference between revisions of "Team:Pasteur Paris/Microbiology week10"
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1. Realize a Mastermix and store it on ice : <br /> | 1. Realize a Mastermix and store it on ice : <br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 113</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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1. Prepare the following tubes :<br /> | 1. Prepare the following tubes :<br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 114</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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3. Add 1 µl of DNA following the number of tubes : <br /><br /> | 3. Add 1 µl of DNA following the number of tubes : <br /><br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 115</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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Follow the Qiagen Extraction gel kit steps with :<br /><br /> | Follow the Qiagen Extraction gel kit steps with :<br /><br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 116</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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1. Use 1/10 volume of NaAc and 2.5 colume of ethanol for each insert : <br /><br /> | 1. Use 1/10 volume of NaAc and 2.5 colume of ethanol for each insert : <br /><br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 117</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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Use the following volumes : <br /> | Use the following volumes : <br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 118</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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2. Digest the plasmid with the following volumes for each sample : <br /> | 2. Digest the plasmid with the following volumes for each sample : <br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 119</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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1. Realize a master mix with : <br /><br /> | 1. Realize a master mix with : <br /><br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 120</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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1. Put all the following reactants in a 1.5 ml Eppendorf and let digest one hour at 37°C : <br /> | 1. Put all the following reactants in a 1.5 ml Eppendorf and let digest one hour at 37°C : <br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 121</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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1. Make two measurements separated of 30 minutes : <br /> | 1. Make two measurements separated of 30 minutes : <br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 122</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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1. Start with tube 2 (8.6 ng/µl in 46 µl) and use the following mix : <br /> | 1. Start with tube 2 (8.6 ng/µl in 46 µl) and use the following mix : <br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 123</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
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1. In a 1.5 ml Eppendorf, put : <br /> | 1. In a 1.5 ml Eppendorf, put : <br /> | ||
<table> | <table> | ||
| − | <caption align="bottom" align="center">Table | + | <caption align="bottom" align="center"><i><p> <U>Table 124</U></p></i></caption> |
<thead> | <thead> | ||
<tr> | <tr> | ||
