Difference between revisions of "Team:Edinburgh OG/Safety"

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                     <h4> Our team has expandED the safety aspect of our project to integrate with our human practices. For detailed discussion of this please click below to go to Human Practices page.</h3>
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                     <h4> Our team has expandED the safety aspect of our project to integrate with our human practices. For detailed discussion of this please click below to go to Human Practices page. <h4>
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                     <p>  Our team has also worked to meet all local, national, international and iGEM safety requirements as well as considering safe experimental design and practice.</p>
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                      <br><a href="https://2016.igem.org/Team:Edinburgh_OG/Human_Practices" class="page-scroll btn btn-default btn-xl sr-button">Human Practices</a>
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                      <br>
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                     <p>  Our team has worked to ensure we meet all local, national, international and iGEM safety requirements. We have also actively considered safe experimental design and practice.</p>
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                      <br>
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                      <br>
 
                     <h4>Meeting Local Policies</h4>
 
                     <h4>Meeting Local Policies</h4>
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                      <br>
 
                     <p>Firstly, each team member has had to abide by University of Edinburgh policies and practices, as governed by the Health and Safety Department Biosafety Unit and has received a lab induction and training in handling of chemicals, sterile techniques, proper disposal of contaminated material and handling of microorganisms.</p>
 
                     <p>Firstly, each team member has had to abide by University of Edinburgh policies and practices, as governed by the Health and Safety Department Biosafety Unit and has received a lab induction and training in handling of chemicals, sterile techniques, proper disposal of contaminated material and handling of microorganisms.</p>
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                      <br>
 
                     <h4>Meeting National and international Policies</h4>
 
                     <h4>Meeting National and international Policies</h4>
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                      <br>
 
                     <p>As a team based in Scotland our work has been conducted in accordance with Scottish, UK and European regulations. All safety guidelines outlined in the Control of Substances Hazardous to Health Regulations 2002 act have been followed and further due to use of Genetically Modified Organisms we have also complied with guidelines stated in the The Genetically Modified Organisms (Contained Use) Regulations 2014
 
                     <p>As a team based in Scotland our work has been conducted in accordance with Scottish, UK and European regulations. All safety guidelines outlined in the Control of Substances Hazardous to Health Regulations 2002 act have been followed and further due to use of Genetically Modified Organisms we have also complied with guidelines stated in the The Genetically Modified Organisms (Contained Use) Regulations 2014
 
                     </p>
 
                     </p>
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                      <br>
 
                     <h4>iGEM Policies</h4>
 
                     <h4>iGEM Policies</h4>
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                      <br>
 
                     <p>We have followed all iGEM policies including completion of the ‘About Our Lab’ and ‘About Our Project’ questionnaires and the final safety form. All organisms used and parts designed are on the white list and are not flagged. Further, none of our parts are found in the Australia group list for export control or the US list of select agents and so we do not have any issues with sending our parts to iGEM.
 
                     <p>We have followed all iGEM policies including completion of the ‘About Our Lab’ and ‘About Our Project’ questionnaires and the final safety form. All organisms used and parts designed are on the white list and are not flagged. Further, none of our parts are found in the Australia group list for export control or the US list of select agents and so we do not have any issues with sending our parts to iGEM.
 
                     </p>
 
                     </p>
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                      <br>
 
                     <h4>Safe Design</h4>
 
                     <h4>Safe Design</h4>
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                      <br>
 
                     <p>All organisms chosen for our project are Generally Recognised As Safe. While this provides some reassurance of safety, safety can certainly not be assumed and this is expanded on within our Human Practises. As such each individual experiment was assessed case by case and conducted with appropriate care and within a suitable laboratory.
 
                     <p>All organisms chosen for our project are Generally Recognised As Safe. While this provides some reassurance of safety, safety can certainly not be assumed and this is expanded on within our Human Practises. As such each individual experiment was assessed case by case and conducted with appropriate care and within a suitable laboratory.
 
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                     <p>Penicillium roqueforti can produce toxins and immunosupressants in certain growth conditions and media. Potential toxicity is not well understood however the organism is generally regarded as safe and has been used commercially and domestically for over 1,000 years.In order to reduce cross contamination between or organisms as well as risks to the students, we use one lab specifically for E. coli and another lab for Rhodoccocus and P. roqueforti. This second laboratory is equiped with level 2 biosafety cabinets which mitigates the risk for the user.</p>
 
                     <p>Penicillium roqueforti can produce toxins and immunosupressants in certain growth conditions and media. Potential toxicity is not well understood however the organism is generally regarded as safe and has been used commercially and domestically for over 1,000 years.In order to reduce cross contamination between or organisms as well as risks to the students, we use one lab specifically for E. coli and another lab for Rhodoccocus and P. roqueforti. This second laboratory is equiped with level 2 biosafety cabinets which mitigates the risk for the user.</p>
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                    <h4> We also integrated the safety aspect of our project to with the human practices. Click below to go to Human Practices page. <h4>
 
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                      <br><a href="https://2016.igem.org/Team:Edinburgh_OG/Human_Practices" class="page-scroll btn btn-default btn-xl sr-button">Human Practices</a>
 
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
  
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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</ul>
 
  
 
</div>
 
</div>

Revision as of 01:57, 20 October 2016

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Safety

Our team has expandED the safety aspect of our project to integrate with our human practices. For detailed discussion of this please click below to go to Human Practices page.


Human Practices



Our team has worked to ensure we meet all local, national, international and iGEM safety requirements. We have also actively considered safe experimental design and practice.



Meeting Local Policies


Firstly, each team member has had to abide by University of Edinburgh policies and practices, as governed by the Health and Safety Department Biosafety Unit and has received a lab induction and training in handling of chemicals, sterile techniques, proper disposal of contaminated material and handling of microorganisms.


Meeting National and international Policies


As a team based in Scotland our work has been conducted in accordance with Scottish, UK and European regulations. All safety guidelines outlined in the Control of Substances Hazardous to Health Regulations 2002 act have been followed and further due to use of Genetically Modified Organisms we have also complied with guidelines stated in the The Genetically Modified Organisms (Contained Use) Regulations 2014


iGEM Policies


We have followed all iGEM policies including completion of the ‘About Our Lab’ and ‘About Our Project’ questionnaires and the final safety form. All organisms used and parts designed are on the white list and are not flagged. Further, none of our parts are found in the Australia group list for export control or the US list of select agents and so we do not have any issues with sending our parts to iGEM.


Safe Design


All organisms chosen for our project are Generally Recognised As Safe. While this provides some reassurance of safety, safety can certainly not be assumed and this is expanded on within our Human Practises. As such each individual experiment was assessed case by case and conducted with appropriate care and within a suitable laboratory.

Penicillium roqueforti, while GRAS, is known to produce mycotoxins that are potentially lethal to humans when it certain growth conditions. Potential toxicity is not well understood however the organism has been widely used in food production for over 1,000 years. To mitigate any risk in genetic modification, whenever possible work was carried out in a laminar flow hood and in a separate laboratory from work conducted by other groups.Further, media in which lowered mycotoxin production has been observed was used for cell culture.

Typical antibiotics are ineffective in selection screening with Penicillium roqueforti however success with phleomycin screens is widely reported. This highly toxic group of glycopeptide antibiotics poses risk to human health however no safer alternative could be found. Phleomycin was used only as a low concentration liquid, within a laminar flow hood and all materials making contact with phleomycin were repeatedly washed and disposed of in cytotoxic bins.

Penicillium roqueforti can produce toxins and immunosupressants in certain growth conditions and media. Potential toxicity is not well understood however the organism is generally regarded as safe and has been used commercially and domestically for over 1,000 years.In order to reduce cross contamination between or organisms as well as risks to the students, we use one lab specifically for E. coli and another lab for Rhodoccocus and P. roqueforti. This second laboratory is equiped with level 2 biosafety cabinets which mitigates the risk for the user.