Difference between revisions of "Team:Vilnius-Lithuania/Methods"

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<h1 class="Raleway" style="text-align:center;">Methods</h1>
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<h4 class="panel-title">1. Plasmid DNA extraction</h4>
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<strong>Materials:</strong><br />
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4 mL of LB medium<br />
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Antibiotic<br />
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Transformed bacteria<br />
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<strong>Process</strong>:<br />
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Transformed bacteria, which contain a plasmid with a target DNA, is placed into laboratory tube with 4 ml of liquid LB medium and specific antibiotic. Bacteria are then placed into the shaker during the night at 37?C. For the plasmid DNA extraction we used GeneJet Plasmid Miniprep Kit, according to manufacturer's <a class="clink" href="https://static.igem.org/mediawiki/2015/b/b1/Vilnius15_PlasmidMiniprep.pdf">instructions.</a>
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<h4 class="panel-title">DNA electrophoresis</h4>
 
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<strong>Materials:</strong><br />
 
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Agarose gel<br />
 
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DNA ladder mix<br />
 
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                                                                        1x TAE buffer<br />
 
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6x Loading Dye<br />
 
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<strong>Process</strong>:<br />
 
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the agarose gel is loaded into electrophoresis system filled with the TAE buffer. The DNA ladder and DNA samples are then loaded into the wells. The gel is run at 120V for 30-40 minutes.
 
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<h1 class="Raleway" style="text-align:center;">Methods</h1>
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<span class="line" style="margin:0 auto;"> </span>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Description'>Description</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Design'>Design</a></br>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Modeling'>Modelling</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Results' class="active">Results</a></br>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Proof'>Proof</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Demonstrate'>Demonstrate</a></br>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Notebook'>Notebook</a>
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<a href='https://2016.igem.org/Team:Vilnius-Lithuania/Safety'>Safety</a>
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<p class="Raleway">bla bla</p>
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Revision as of 02:21, 20 October 2016

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