Difference between revisions of "Team:Bulgaria/Part"
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<li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/LabBook">lab book</a></li> | <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/LabBook">lab book</a></li> | ||
<li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Safety">safety</a></li> | <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Safety">safety</a></li> | ||
| − | <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/ | + | <li class="sub-item"><a href="https://2016.igem.org/Team:Bulgaria/Protocols">protocols</a></li> |
</ul> | </ul> | ||
Latest revision as of 02:36, 20 October 2016
This part is composed of K731500 ( (lacI-lacIq and a tac promoter) followed by a CDS of CASH1 optimized for expression in E. coli. +
GAATTCGCGGCCGCTTCTAGtcactgcccgctttccagtcgggaaacctgtc gtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgt attgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctga ttgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctgg tttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatata acatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcacca acgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgat cgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcat ggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatc ggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagac gcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacc caatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaa ataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaa cattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggata gttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgct ttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcac ccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtg cagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgcc agttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgctt ccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcg ggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgtt actggtttcacattcaccaccctgaattgactctcttccgggcgctatcatg ccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcat cggctcgtataatgtgtgggaattgtgagcggataacaattccctcatgaaa taattttgttttactagagtcacacaggaaagtactag
ATGCCATACGAGAAACATGTTGAGCAGACAGTTGTGGAGAAAACGGAGCAGCC GGGCCATTCCAGCACCCACCATGCTCCCGCGCAGCGCACCGTGGCCCGTGAGCAAGA AGAGGTCGTCCATAAGGAGTTTACTCATACTGATATTCGTGTTCCCCATATCGACGC GCCACCACCGATTATTGCGGCATCTGCTGTTGGATTGGCCGAGGAAATCGTTAGTCA CGGGTTTCAAGCCTCAGCGGCTCGTATCTCCGGCGCATCCACGGAGGTGGACATGCG CCCTAGCCCTAAATTGGCCGAAGAGGCTCGTCGCGACGCAGAGCGTTACCAAAAAGA ACACGAAATGATTAACCGTCAAGCTGAGGCCACGTTACAGAAAAAAGCGGAGGAGTA CCGTCACCAGACCGAGGCCGAAGCCGAGAAGATTCGTCGCGAGTTAGAGAAACAGCA CGAACGCGATATTCAATTCCGTAAAGACCTGATCGACCAGACCATTGAAAAACAAAA GCGCGAGGTAGATCTTGAAGCAAAGATGGCAAAACGCGAGCTTGACCGCGAAGCACA GTTAGCAAAAGAAGCCCTGGAACGTAGCCGTATGGCCACAAACGTGGAGGTGACGCT TGATACGGCGGCAGGTCATACTGTATCTGGGGGCACTACAGTCTCCTCGGTTGATAA AGTCGAAACAGTTCGTGAACGCAAACATCATTAATAATTGATTAAcatcaaataaaa cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttattac tagtagcggccgctgcag
This part is composed of K731500 ( (lacI-lacIq and a tac promoter) followed by a CDS of MASH optimized for expression in E. coli. +
GAATTCGCGGCCGCTTCTAGtcactgcccgctttccagtcgggaaacctgtcgt gccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattg ggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgccc ttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgcccc agcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctg tcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccg gactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagc atcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccg gacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcga gtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaat gggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacg cccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctgg tcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagca atggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgc gcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctacc atcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcg acaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagc aacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagc tccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcc tggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgaca tcgtataacgttactggtttcacattcaccaccctgaattgactctcttccggg cgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaa ttaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctc atgaaataattttgttttactagagtcacacaggaaagtactag
ATGGTTTCGTTGCGTTTAGGTGATTTCCGCTCGCAGCCAAGTTTACGTAGCGTGC CAGCGAGCTTAACTTCCCGCAGTCAGGCGTTCAGCCTTCAGGAGATTGCAGCGCG CGCGGGGGTAGTACTGCGTGGCGTGCAACAACAGTTCCGTAACGTAACGGGAGTG AATGCTGCCCCGGTGGTGGCCTTTGACAACGGCTCAGTCCTTTATTCTGAGCGCA TCCATAGCCAATCATCTCAAAAGCAGGCCCCCACAACAGTCCCCACAGGCAGCGT CAGCAATAGTCCTCAACCGGAGGGGAAAGCCAACGAAGCCGCCGAGCGTGCCAAA CAATTTATGAACCCACCCGTAGCCCCAATGGACCCAGTCGACAAAAATGAGTTCG TGGCTATGCCCGAGATGGGGCGCTCCAACGGAAATGGCGAGAACAAGCAGGCAGC GGACTTTATGAAGAATCAGGGCGATACTGACATGGACTCACAGTACGCCCCAGAC TCCAGTAAGAACACGAAGTCTGTCCCGACGAAGGAAATCGTGGCAGAGGACGGAA GTATGTCTATTGAGGATATTAAAAAAGCGACGCAGGTTACTCCAGGGGTCGCGGT TAAAAATGAGGGGGTATAATAATTGATTAAcatcaaataaaacgaaaggctcagt cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta gagtcacactggctcaccttcgggtgggcctttctgcgtttattactagtagcgg ccgctgcag
This part is composed of K731500 ( (lacI-lacIq and a tac promoter) followed by a CDS of RvLEAM optimized for expression in E. coli. +
GAATTCGCGGCCGCTTCTAGtcactgcccgctttccagtcgggaaacctgtcgtg ccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattggg cgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttc accgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagca ggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttc ggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcg gtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcag tgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggc actccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatat ttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgcta acagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgt accgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatca agaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggt catccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtg caccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacg ctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcg cgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgc cagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttcc actttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaa cggtctgataagagacaccggcatactctgcgacatcgtataacgttactggttt cacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcga aaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatg tgtgggaattgtgagcggataacaattccctcatgaaataattttgttttactag agtcacacaggaaagtactag
ATGGTTTCGTTGCGTTTAGGTGATTTCCGCTCGCAGCCAAGTTTACGTAGCGTGC CAGCGAGCTTAACTTCCCGCAGTCAGGCGTTCAGCCTTCAGGAGATTGCAGCGCG CGCGGGGGTAGTACTGCGTGGCGTGCAACAACAGTTCCGTAACGTAACGGGAGTG AATGCTGCCCCGGTGGTGGCCTTTGACAACGGCTCAGTCCTTTATTCTGAGCGCA TCCATAGCCAATCATCTCAAAAGCAGGCCCCCACAACAGTCCCCACAGGCAGCGT CAGCAATAGTCCTCAACCGGAGGGGAAAGCCAACGAAGCCGCCGAGCGTGCCAAA CAATTTATGAACCCACCCGTAGCCCCAATGGACCCAGTCGACAAAAATGAGTTCG TGGCTATGCCCGAGATGGGGCGCTCCAACGGAAATGGCGAGAACAAGCAGGCAGC GGACTTTATGAAGAATCAGGGCGATACTGACATGGACTCACAGTACGCCCCAGAC TCCAGTAAGAACACGAAGTCTGTCCCGACGAAGGAAATCGTGGCAGAGGACGGAA GTATGTCTATTGAGGATATTAAAAAAGCGACGCAGGTTACTCCAGGGGTCGCGGT TAAAAATGAGGGGGTATAATAATTGATTAAcatcaaataaaacgaaaggctcagt cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta gagtcacactggctcaccttcgggtgggcctttctgcgtttattactagtagcgg ccgctgcag
This part is composed of K731500 ( (lacI-lacIq and a tac promoter) followed by a CDS of SAHS2 optimized for expression in E. coli. +
GAATTCGCGGCCGCTTCTAGtcactgcccgctttccagtcgggaaacctgtcgtg ccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattggg cgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttc accgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagca ggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttc ggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcg gtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcag tgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggc actccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatat ttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgcta acagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgt accgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatca agaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggt catccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtg caccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacg ctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcg cgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgc cagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttcc actttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaa cggtctgataagagacaccggcatactctgcgacatcgtataacgttactggttt cacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcga aaggttttgcgccattctactagaggttgacaattaatcatcggctcgtataatg tgtgggaattgtgagcggataacaattccctcatgaaataattttgttttactag agtcacacaggaaagtactag
ATGGCAGATGACGCAGCTCACGAGGAAGGGGTGGAGTGGACGGGTAAGCCGTGGA TGGGAAAATGGGAGTCAGATCCTTCTAAGGATGAGAATGTAGAAGAGTTTAAGAA AAAATTGCAGTTACCTATGTCCCATTCGGAAATGAACAAGAACTCCAAAGTCTGG ATTCACCACTATAAAAAAGGCGACGAATACCATCATAAGATTATTATCAATGACG CGCATTACAAAAATGATATCGTGTTCAAATTGGGACAAGAGAGTGCCGGGTCGTA TAATGGCTCATCATTCTCCGTGAAATACGAAGACAAAGACGGTGCTTTAGTTGGA AGCGTACACTACACGGGTACAAAAGAGCAAAGTCTTGATAAAACCATCAATAATG TATTCAAGCTGGAGGGCGACCACCTGGTGAAGACGTCCACCATTGAGGGGGTTAC CATGAAGCGCCACTACAACAAACGTCAGTAATAATTGATTAAcatcaaataaaac gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat tactagtagcggccgctgcag
This part is composed of K731500 ( (lacI-lacIq and a tac promoter) followed by a CDS of SAHS2 optimized for expression in E. coli. In addition, a periplasmic secretion signal peptide (KKLLFAIPLVVPFYSHS) is added at the N terminus.+
GAATTCGCGGCCGCTTCTAGtcactgcccgctttccagtcgggaaacctgtcgt gccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattg ggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgccc ttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgcccc agcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctg tcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccg gactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagc atcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccg gacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcga gtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaat gggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacg cccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctgg tcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagca atggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgc gcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctacc atcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcg acaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagc aacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagc tccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcc tggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgaca tcgtataacgttactggtttcacattcaccaccctgaattgactctcttccggg cgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaa ttaatcatcggctcgtataatgtgtgggaattgtgagcggataacaattccctc atgaaataattttgttttactagagtcacacaggaaagtactag
ATGAAAAAACTGCTGTTCGCGATTCCGCTGGTGGTGCCGTTCTATAGCCATAGC GCAGATGACGCAGCTCACGAGGAAGGGGTGGAGTGGACGGGTAAGCCGTGGATG GGAAAATGGGAGTCAGATCCTTCTAAGGATGAGAATGTAGAAGAGTTTAAGAAA AAATTGCAGTTACCTATGTCCCATTCGGAAATGAACAAGAACTCCAAAGTCTGG ATTCACCACTATAAAAAAGGCGACGAATACCATCATAAGATTATTATCAATGAC GCGCATTACAAAAATGATATCGTGTTCAAATTGGGACAAGAGAGTGCCGGGTCG TATAATGGCTCATCATTCTCCGTGAAATACGAAGACAAAGACGGTGCTTTAGTT GGAAGCGTACACTACACGGGTACAAAAGAGCAAAGTCTTGATAAAACCATCAAT AATGTATTCAAGCTGGAGGGCGACCACCTGGTGAAGACGTCCACCATTGAGGGG GTTACCATGAAGCGCCACTACAACAAACGTCAGTAATAATTGATTAAcatcaaa taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct gcgtttattactagtagcggccgctgcag
