Line 249: | Line 249: | ||
<p1>From the raw data of absorbance and glucose meter readings, the actual concentrations were determined using a calibration curve created by measuring absorbance and glucose levels in known dilutions. The experiments performed to generate the two calibration curves for cell concentration and glucose concentration is outlined in the Methods section. Using the two calibration curves, we converted the raw experimental measures to their actual values before using them to estimate model parameters. </p1> | <p1>From the raw data of absorbance and glucose meter readings, the actual concentrations were determined using a calibration curve created by measuring absorbance and glucose levels in known dilutions. The experiments performed to generate the two calibration curves for cell concentration and glucose concentration is outlined in the Methods section. Using the two calibration curves, we converted the raw experimental measures to their actual values before using them to estimate model parameters. </p1> | ||
<br> | <br> | ||
− | <img src="https://static.igem.org/mediawiki/2016/4/4f/BroadRun_Testing_fig13.png" alt=lskf" style="width: | + | <img src="https://static.igem.org/mediawiki/2016/4/4f/BroadRun_Testing_fig13.png" alt=lskf" style="width:1046px;height:351px;"> |
<br> | <br> | ||
Line 266: | Line 266: | ||
− | <img src="https://static.igem.org/mediawiki/2016/1/1e/BroadRun_Testing_fig14.png" alt="lskf" style="width: | + | <img src="https://static.igem.org/mediawiki/2016/1/1e/BroadRun_Testing_fig14.png" alt="lskf" style="width:1045px;height:396px;"> |
Line 272: | Line 272: | ||
The first graph is a double-reciprocal Line-Weaver Burk plot. From the slope and intercept, the maximum specific growth rate and the saturation constant are estimated. </p1> | The first graph is a double-reciprocal Line-Weaver Burk plot. From the slope and intercept, the maximum specific growth rate and the saturation constant are estimated. </p1> | ||
− | <img src="https://static.igem.org/mediawiki/2016/5/53/BroadRun_Testing_fig15.png" alt="lskf" style="width: | + | <img src="https://static.igem.org/mediawiki/2016/5/53/BroadRun_Testing_fig15.png" alt="lskf" style="width:1045px;height:788px;"> |
<p1>Figure 15. Log plot of exponential cell growth. | <p1>Figure 15. Log plot of exponential cell growth. | ||
Line 282: | Line 282: | ||
<h3>Comparing Growth Characteristics</h3> | <h3>Comparing Growth Characteristics</h3> | ||
− | + | ||
<p1>In addition to providing estimates of model parameters, the graphs in the preceding section enables comparison of growth characteristics. The maximum specific growth rates are 0.017 per hour and 0.020 per hour for the genetically modified and wild type yeast in glucose, respectively, indicating that the genetically modified yeast have slightly lower growth rates. Comparison of the genetically modified yeast performance in regards to substrate, show a lower growth rate of 0.007 per hour for the starch substrate compared to the 0.017 per hour for the glucose substrate. | <p1>In addition to providing estimates of model parameters, the graphs in the preceding section enables comparison of growth characteristics. The maximum specific growth rates are 0.017 per hour and 0.020 per hour for the genetically modified and wild type yeast in glucose, respectively, indicating that the genetically modified yeast have slightly lower growth rates. Comparison of the genetically modified yeast performance in regards to substrate, show a lower growth rate of 0.007 per hour for the starch substrate compared to the 0.017 per hour for the glucose substrate. | ||
</p1> | </p1> | ||
<p1>The growth yield Yx/s is 5.07 and 5.91 million cells per mg of glucose consumed, for the genetically modified yeast and the wild type yeast, respectively. This tells us that during the exponetial growth phase for the same amount of glucose consumed as the wild type, the genetically modified yeast reproduce slower. </p1> | <p1>The growth yield Yx/s is 5.07 and 5.91 million cells per mg of glucose consumed, for the genetically modified yeast and the wild type yeast, respectively. This tells us that during the exponetial growth phase for the same amount of glucose consumed as the wild type, the genetically modified yeast reproduce slower. </p1> | ||
− | + | ||
<p1>The saturation constants relating to glucose limiting growth are 17.3 and 5.4 mg/dL glucose for the genetically modified yeast and the wild type yeast, respectively. The higher saturation constant value for the genetically modified yeast indicated that the glucose-limiting growth kinetics begins at higher glucose concentrations, and is consistent with the higher glucose demands of the genetically modified yeast. </p1> | <p1>The saturation constants relating to glucose limiting growth are 17.3 and 5.4 mg/dL glucose for the genetically modified yeast and the wild type yeast, respectively. The higher saturation constant value for the genetically modified yeast indicated that the glucose-limiting growth kinetics begins at higher glucose concentrations, and is consistent with the higher glucose demands of the genetically modified yeast. </p1> | ||
Latest revision as of 02:53, 20 October 2016
.MathJax nobr>span.math>span{border-left-width:0 !important};
Testing
Testing in Known Starch solutions
Short Term Testing
Long Term Starch Degradation Testing
Testing In Industrial Water Samples
- 1) aeration basin
- 2) secondary clarifier
- 3) primary clarifier to the equalization basin
- 4) thickener to primary clarifier
- 5) thickener to dry broke
- 6) primary clarifier to dry broke