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Revision as of 03:02, 20 October 2016
Composite Part
The overall goal of our project was to develop an organism capable of reducing excess nitrate in local waterways. However, we don't want our genetically engineered machine to remain in the environment after it has sufficiently reduced nitrate levels. To ensure the safety of our system, we developed this composite biobrick to act as our kill switch. In Jurassic Park, Henry Wu developed a "Lysine Contingency" to ensure the dinosaurs could not survive without sufficient lysine supplemented in their food. Similarly, we designed a "Serine Contingency" kill switch for our bacteria. (Although it is our hope that our kill switch mechanism will function more effectively than the fictional one described in the novel.)
The mechanism of our kill switch is depicted here, where in the presence of nitrate, serine is expressed, and the cell is able to survive. Once it has 'done its job', the nitrate will no longer be present, and so PyeaR and consequently serA will no longer be expressed.You can view more information and characterization results on the part page: K2086002