Difference between revisions of "Team:UNebraska-Lincoln/Parts"

 
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{{UNebraska-Lincoln}}
 
 
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<link rel="stylesheet" href="https://2016.igem.org/Team:UNebraska-Lincoln/css/Safetymain?action=raw&amp;ctype=text/css"/>
  
  
  
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background-image: url("https://static.igem.org/mediawiki/2016/4/4a/T--UNebraska-Lincoln--blue.png");
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padding-left: 200px;
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
  
  
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<link rel="stylesheet" href="https://2016.igem.org/Team:UNebraska-Lincoln/css/NavigationMenu?action=raw&amp;ctype=text/css" type="text/css" media="screen"/>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln">
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<img src="https://static.igem.org/mediawiki/2016/0/02/T--UNebraska-Lincoln--home2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Home</span>
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<span class="sdt_descr"></span>
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<li style="background-color:#4c5c96;">
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<a  href="https://2016.igem.org/Team:UNebraska-Lincoln/Team">
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<img src="https://static.igem.org/mediawiki/2016/c/cb/T--UNebraska-Lincoln--TeamStock2.png" alt=""/>
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<span class="sdt_wrap">
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<span class="sdt_link"  >Team</span>
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<span class="sdt_descr"></span>
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</span>
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</a>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/The_drawing_board">
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<img src="https://static.igem.org/mediawiki/2016/1/11/T--UNebraska-Lincoln--Blueprints2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Blueprints</span>
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<span class="sdt_descr"></span>
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</span>
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</a>
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<div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/background">Background</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/modeling">Modeling</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/design">Design</a>
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<h5>Note</h5>
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<li style="background-color:#4c5c96;">
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory">
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<img src="https://static.igem.org/mediawiki/2016/a/a3/T--UNebraska-Lincoln--Laboratory2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link" style="">Laboratory</span>
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<span class="sdt_descr"></span>
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                            <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/experiments">Experiments</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/lab_manual">Lab Notebook</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/safety_cases">Safety Cases</a>
  
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</div>
  
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</span>
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</a>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/beyond_the_bench">
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<img src="https://static.igem.org/mediawiki/2016/1/1a/T--UNebraska-Lincoln--beyondthebench2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Outreach</span>
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<span class="sdt_descr"></span>
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                            <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/human_practices">Human Practices</a>
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                                                        <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Integrated_Practices">Integrated Practices</a>
 +
<a href="https://2016.igem.org/Team:UNebraska-Lincoln/collaboration">Collaboration</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/industry">Industry</a>
  
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</div>
  
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</span>
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</a>
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</li>
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<li style="background-color:#4c5c96;">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln">
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<img src="https://static.igem.org/mediawiki/2016/e/ef/T--UNebraska-Lincoln--thanks2.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Thanks!</span>
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<span class="sdt_descr"></span>
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                <div class="sdt_box">
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/sponsors">Sponsors</a>
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<a href="https://2016.igem.org/Team:UNebraska-Lincoln/attributions">Attributions</a>
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</div>
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</span>
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</a>
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</li>
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</ul>
  
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<div class="drawing board" style=" background-color: #2e3141; padding-top:50px;">
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<section><img src="https://static.igem.org/mediawiki/2016/0/0f/T--UNebraska-Lincoln--design.png" align="middle" style="width:100%; height:100%; padding-bottom: 30px " alt="image"/></section>
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                                    <section>
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<section>
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    <div style="transform: scale(1);margin-left:150px; margin-right:150px">                   
  
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<h2 class="major">Parts</h2>
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<p><center><img src="https://static.igem.org/mediawiki/2016/1/15/T--UNebraska-Lincoln--parts.png" style="width:40%;height:auto;" transform:scale(0.2)></center></p>
  
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<p><h2> Basic Parts</h2></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2086002"><span class="image left"><img src="https://static.igem.org/mediawiki/2016/7/7a/T--UNebraska-Lincoln--yellowgear.png
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" alt="" style="width:100px;height:auto;" transform:scale(1.0) /></span></a><font color="silver"><a href="http://parts.igem.org/Part:BBa_K2086002"><h2>Nitrate Reductase (napDABC)</h2></a>A coding sequence for the synthetic operon that encodes for four of the proteins that make up the periplasmic nitrate reductase. This protein comprise one of the pathways native to E. coli that is responsible for reducing nitrate ions to nitrite ions. After researching the protein, we determined the genes essential for the synthesis of the reductase. Portions of the nap operon isolated from <i>E. coli</i>, as well as some parts which we ordered from IDT. The full details of this basic part can be found <a href="http://parts.igem.org/Part:BBa_K2086000">Here</a></font></font>
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<p></p>
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<p></p>
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<p></p>
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<p></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2086001"><span class="image left"><img src="https://static.igem.org/mediawiki/2016/5/52/T--UNebraska-Lincoln--greengear.png
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" alt="" style="width:100px;height:auto;" transform:scale(1.0) /></span></p><font color="silver"><h2>serA</h2></a>
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This gene codes for D-3-phosphoglycerate dehydrogenase, an enzyme catalyzing the committed step in serine biosynthesis. This part was designed and isolated in order to complement an auxotrophic strain of <i>E. coli</i> which is unable to produce serine. This part can be complemented with any BioBrick-compatible promoter for the design of a kill-switch. We used this basic part in the formation of our composite part, a PyeaR-serA kill switch. The full details and characterization of this basic part can be found <a href="http://parts.igem.org/Part:BBa_K2086001">Here</a></font></font>
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<p></p>
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<p></p><p></p>
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<p><a href="https://2016.igem.org/Team:UNebraska-Lincoln/Composite_Part"><h2>Composite Part</h2></a></p>
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<p></p>
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<a href="http://parts.igem.org/Part:BBa_K2086002"><span class="image left"><img src="https://static.igem.org/mediawiki/2016/6/6f/T--UNebraska-Lincoln--bluegear.png
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" alt="" style="width:100px;height:auto;" transform:scale(1.0) /></span></a><font color="silver"><a href="http://parts.igem.org/Part:BBa_K2086002"><h2>Nitrate-Sensitive Kill Switch (PyeaR-SerA)</h2></a>
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A composite part designed to act as a nitrate-sensitive kill-switch. The yeaR promoter was originally used by the Edinburgh 2009 iGEM team and was shown to be nitrate sensitive. By complementing the promoter with the serA basic part, it’s capable of acting as a kill-switch. The kill switch complements a ΔserA strain of E. coli under high nitrate concentration conditions, but causes the E. coli to die under low nitrate conditions.  The full description of this composite part can be found <a href="https://2016.igem.org/Team:UNebraska-Lincoln/Composite_Part">Here</a></font>
  
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<h5>What information do I need to start putting my parts on the Registry?</h5>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
  
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<p>
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</section>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<div class="inner">
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<h2 class="major">How did this work?</h2>
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<center><a href="https://2016.igem.org/Team:UNebraska-Lincoln/laboratory"><img src="https://static.igem.org/mediawiki/2016/9/9c/T--UNebraska-Lincoln--tothelab.png"  style="width:60%;height:auto;" transform:scale(0.2)></a></center>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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<h5>Part Table </h5>
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/skelmin?action=raw&amp;ctype=text/javascript"></script>
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<div class="highlight">
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/jquery1?action=raw&amp;ctype=text/javascript"></script>
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/jqueryscrollex?action=raw&amp;ctype=text/javascript"></script>
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/util?action=raw&amp;ctype=text/javascript"></script>
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<groupparts>iGEM16_UNebraska-Lincoln</groupparts>
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<script src="https://2016.igem.org/Team:UNebraska-Lincoln/libraries/main?action=raw&amp;ctype=text/javascript"></script>
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Latest revision as of 03:06, 20 October 2016

image

Parts

Basic Parts

Nitrate Reductase (napDABC)

A coding sequence for the synthetic operon that encodes for four of the proteins that make up the periplasmic nitrate reductase. This protein comprise one of the pathways native to E. coli that is responsible for reducing nitrate ions to nitrite ions. After researching the protein, we determined the genes essential for the synthesis of the reductase. Portions of the nap operon isolated from E. coli, as well as some parts which we ordered from IDT. The full details of this basic part can be found Here

serA

 This gene codes for D-3-phosphoglycerate dehydrogenase, an enzyme catalyzing the committed step in serine biosynthesis. This part was designed and isolated in order to complement an auxotrophic strain of E. coli which is unable to produce serine. This part can be complemented with any BioBrick-compatible promoter for the design of a kill-switch. We used this basic part in the formation of our composite part, a PyeaR-serA kill switch. The full details and characterization of this basic part can be found Here

Composite Part

Nitrate-Sensitive Kill Switch (PyeaR-SerA)

 A composite part designed to act as a nitrate-sensitive kill-switch. The yeaR promoter was originally used by the Edinburgh 2009 iGEM team and was shown to be nitrate sensitive. By complementing the promoter with the serA basic part, it’s capable of acting as a kill-switch. The kill switch complements a ΔserA strain of E. coli under high nitrate concentration conditions, but causes the E. coli to die under low nitrate conditions. The full description of this composite part can be found Here