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<p style="text-align:center;">4 NADH + O<sub>2</sub> → 4 NAD<sup>+</sup> + 2 H<sub>2</sub>O + 4 e<sup>-</sup></p> | <p style="text-align:center;">4 NADH + O<sub>2</sub> → 4 NAD<sup>+</sup> + 2 H<sub>2</sub>O + 4 e<sup>-</sup></p> | ||
− | <p>We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and a modified RBS sequence based on the <a href="http://parts.igem.org/Part:BBa_K1123010"> BBa_K1123010 RBS </a> used by TU Eindhoven 2013 under the FNR promoter. Our version has been optimized to reduce homodimer formation during PCR (<a href="http://parts.igem.org/Part:BBa_K2182001">BBa_K2182004</a> .</p> | + | <p>We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and a modified RBS sequence based on the <a href="http://parts.igem.org/Part:BBa_K1123010"> BBa_K1123010 RBS </a> used by TU Eindhoven 2013 under the FNR promoter. Our version has been optimized to reduce homodimer formation during PCR (<a href="http://parts.igem.org/Part:BBa_K2182001">BBa_K2182004</a>).</p> |
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Latest revision as of 22:39, 6 November 2016
basic parts
NADH Oxidase
This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:
4 NADH + O2 → 4 NAD+ + 2 H2O + 4 e-
We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and a modified RBS sequence based on the BBa_K1123010 RBS used by TU Eindhoven 2013 under the FNR promoter. Our version has been optimized to reduce homodimer formation during PCR (BBa_K2182004).