Difference between revisions of "Team:Northeastern/Composite Part"

(Swapped images (hopefully))
 
Line 35: Line 35:
 
                 <div class="content">
 
                 <div class="content">
 
                     <center>
 
                     <center>
−
                         <img src="https://static.igem.org/mediawiki/2016/c/cd/T--Northeastern--parts_blh_pr_02.PNG">
+
                         <img src="https://static.igem.org/mediawiki/2016/1/12/T--Northeastern--nox_03.PNG">
 
                     </center>
 
                     </center>
 
                 </div>
 
                 </div>
Line 59: Line 59:
 
                 <div class="content">
 
                 <div class="content">
 
                     <center>
 
                     <center>
−
                         <img src="https://static.igem.org/mediawiki/2016/1/12/T--Northeastern--nox_03.PNG">
+
                         <img src="https://static.igem.org/mediawiki/2016/c/cd/T--Northeastern--parts_blh_pr_02.PNG">
 
                     </center>
 
                     </center>
 
                 </div>
 
                 </div>

Latest revision as of 22:40, 6 November 2016

composite parts

Blh+PR operon

We placed proteorhodopsin downstream of a composite part designed UNITN-Trento 2015 that expresses β-carotene 15,15’-dioxygenase under the J23100 constitutive Anderson promoter. This part both synthesizes proteorhodopsin and the blh enzyme that cleaves β-carotene into the retinal chromophore needed for PR to operate as a light-driven proton pump. This is a simpler part than UNITN-Trento’s composite BBa_K1604025 but requires external β-carotene to function.

Nox translational unit

In order to express and characterise Nox, the novel his-tagged protein coding gene was place under the J23100 promoter. This allows constitutive expression of NADH oxidase enzyme that can be easily purified from cell lysates using ELISA.