Difference between revisions of "Template:Rice"

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{{CSS/Main}}
 
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<html>
 
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<link href='https://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,600,700' rel='stylesheet' type='text/css'>
 
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<style>
 
−
 
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      /*-- Remove borders, float content left and remove padding/margin */
 
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      #globalWrapper, #content{
 
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                width: 100%;
 
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}
 
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                #top_menu_14 { padding-left:11%}
 
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</style>
 
  
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<!-- Declare that you are going to use html code instead of wiki code -->
 
 
<html>
 
<html>
 +
<link href="https://2014.igem.org/Team:LMU-Munich/home.css?action=raw&ctype=text/css" rel="stylesheet"  type="text/css">
 +
<div id="home">
 +
<div id="home-logo">
 +
<a href="https://igem.org/Main_Page" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2014/8/8a/LMU_IGEMLogo.png" />
 +
</a>
 +
</div>
  
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<!-- Start of CSS-->
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<div id="twopager">
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<style type="text/css">
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<a href="https://static.igem.org/mediawiki/2014/2/29/LMU14_2pager-final.pdf" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2014/3/37/LMU14_2pager.png" />
 +
</a>
 +
</div>
 +
    <img id="skyline" src="https://static.igem.org/mediawiki/2014/9/97/LMU14_home_completeskyline.jpg"/>     
 +
</div>
 +
<div id="lmu-content2">
 +
    <div id="tiles">
 +
        <div class="row">
 +
            <div class="col-xs-12 col-md-4">
 +
                <a href="https://2014.igem.org/Team:LMU-Munich/Project/Problem">
 +
<img src="https://static.igem.org/mediawiki/2014/8/82/LMU14_home_button_problem.png" />
 +
</a>
 +
            </div>
 +
            <div class="col-xs-12 col-md-4">
 +
<a href="https://2014.igem.org/Team:LMU-Munich/Project/Bakillus">
 +
                <img src="https://static.igem.org/mediawiki/2014/b/b1/LMU14_home_button_strategy.png" />
 +
</a>
 +
            </div>
 +
            <div class="col-xs-12 col-md-4">
 +
<a href="https://2014.igem.org/Team:LMU-Munich/Results">
 +
                <img src="https://static.igem.org/mediawiki/2014/8/8e/LMU14_home-button-results.png" />
 +
</a>
 +
            </div>
 +
        </div>
 +
    </div>
 +
<div id="home-abstract">
 +
</html>
  
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/* PAGE LAYOUT */
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<br/>
  
 +
=Abstract=
 +
Increasing bacterial resistance to classical antibiotics remains a serious threat and urges the development of novel pathogen-killing strategies. Exploiting bacterial communication mechanisms such as quorum sensing is a promising strategy to specifically target certain pathogens. The major aim of this project is the introduction of a genetic circuit enabling ''Bacillus subtilis'' to actively detect, attach to, and eventually kill ''Staphylococcus aureus'' and ''Streptococcus pneumoniae''.
  
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/* Change Background color*/
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Initially, we will introduce the autoinducer-sensing two-component systems of ''S. aureus'' and ''S. pneumoniae'' into ''B. subtilis''.  to create a pathogen-detecting strain. By utilizing quorum sensing-dependent promoters, we will then trigger pathogen-killing strategies like the production of antimicrobial peptides or biofilm degradation. As a safety measure a delayed suicide-switch guarantees non-persistence of genetically modified ''B. subtilis'' in the absence of pathogens. We envision the use of BaKillus as a smart, cheap and simple-to-use medical device for diagnostics and targeted treatment of multiresistant super bugs.
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body {
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background-color: #1b1c3a;
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              }
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/* Creates a container that will wrap all of the content inside your wiki pages. */
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<html>
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#mainContainer { 
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</div>
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width: 100%;
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<div id="home-footer-wrapper">
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float:left;
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<div id="home-footer">
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margin-left: 0px;
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                                margin-right: 0px;
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margin-bottom: 10px;
+
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background-color: #FFFFFF;
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border-bottom: 0px solid #565656;
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border-right: 0px solid #565656;
+
−
border-left: 0px solid #565656;
+
−
border-top: 0px solid #565656;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
+
  
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}
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</div>
 +
</div>
 +
</div>
 +
</html>
  
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/* Creates the container for the menu */
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<html><script>initiateNavigation("none");</script></html>
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#menuContainer  {
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float: left;
+
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width: 100%;
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margin:0;
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padding:0;
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background-color: #1b1c3a;
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margin-top: 0px;
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z-index:0;
+
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            }
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−
 
+
−
/* Creates the container for the content */
+
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#contentContainer {
+
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padding-top:20px;
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padding-bottom: 20px;
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padding-right:20px;
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padding-left: 20px;
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margin-bottom: 10px;
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margin-left: 20px;
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margin-right: 20px;
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width: 92%;
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float: left;
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background-color: #FFFFFF;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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}
+
−
 
+
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/*Set up height place holder for the title*/
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#titleContainer {
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                        width: 100%;
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height:auto;
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overflow:hidden;
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margin: 0px;
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                        background-color: #1b1c3a;
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text-align:center;
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                        text-shadow: 2px 2px #000000;
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}
+
−
 
+
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/*Set up height place holder for the banner*/
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#bannerContainer {
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                        width: 100%;
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                        height: 75%;
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/*overflow:hidden;*/
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margin: 0px;
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text-align:center;
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} */
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−
 
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/*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */
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/*Change the styling of text for everything inside main container*/
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#mainContainer p {
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font-size: 15px;
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color: #000000;
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}
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/*This changes the color and font family */
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#contentContainer h1, h2, h3, h4, h5, h6 {
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color: #565656;
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border-bottom: none;
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font-weight: bold;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
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margin-top:0px;
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}
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−
 
+
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/*Style of the links - links are different inside the menu */
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#contentContainer a {
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font-weight: bold;
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color: #000000;
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                                font-size: 100%;
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}
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−
 
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/* Styling links on hover- links are different inside the menu */
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/* #contentContainer a:hover {
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color: #59BF92;
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} */
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−
 
+
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/*Change the styling of tables */
+
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      #contentContainer table {
+
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    border: 1px solid #565656;
+
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    border-collapse: collapse;
+
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    width: 90%
+
−
    margin: auto;
+
−
    margin-bottom: 0px;
+
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    margin-top: 0px;
+
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    margin-right: 0px;
+
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    margin-left: 0px;
+
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    }
+
−
 
+
−
/*Change the styling of table cells*/
+
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    #contentContainer  td {
+
−
    padding: 0px;
+
−
    border: 1px solid #565656;
+
−
    border-collapse: collapse;
+
−
    vertical-align: text-top;
+
−
                             
+
−
    }
+
−
 
+
−
/*Change the styling of table headers */
+
−
    #contentContainer th {
+
−
    background-color: #FFFFFF;
+
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    padding: 0px;
+
−
    border: 1px solid #565656;
+
−
    border-collapse: collapse;
+
−
    vertical-align: text-top;
+
−
    }
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−
 
+
−
 
+
−
 
+
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/*MENU STYLING */
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#menuContainer {
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text-align: center;
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                                        padding-top: 10px;
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                                        padding-bottom: 20px;
+
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    }
+
−
 
+
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#menuContainer > ul a {
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color: #FFF;
+
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}
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#menuContainer > ul {
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list-style: none;
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margin-left: 0;
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padding-top: 10px;
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text-align: center;
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                                        width: 100%
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}
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#menuContainer > ul ul {
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list-style: none;
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margin-left: 0%;
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padding-top: 0;
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text-align: center;
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                                        width: 100%
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}
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#menuContainer > ul li {
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position: relative;
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text-align: center;
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}
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#menuContainer > ul a {
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padding: 10px 8px;
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display: block;
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text-decoration: none;
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font-family: "Helvetica Neue", Helvetica, sans-serif;
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font-size: 155%;
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}
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#menuContainer > ul a:hover {
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background: #000;
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background-color: #3b3d63;
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}
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−
 
+
−
 
+
−
/* Level 1 Drop Down Menu */
+
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#menuContainer > ul > li {
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−
display: inline-block;
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vertical-align: top;
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margin-left: -4px; /* solve the 4 pixels spacing between list-items */
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}
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#menuContainer > ul > li:first-child {
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margin-left: 0;
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}
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#menuContainer > ul > li > a {}
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#menuContainer > ul > li > a:hover {}
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/* Level 2 */
+
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#menuContainer > ul > li > ul {
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text-align: left;
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−
width: auto; /* change auto value with 200px if you want a bigger menu */
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−
display: none;
+
−
background-color: #1b1c3a;
+
−
position: absolute;
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top: 100%;
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left: 0;
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                                        margin-top: 0px;
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−
z-index: 9999999; /* if you have YouTube iframes, is good to have a bigger z-index so the video can appear above the video */
+
−
}
+
−
#menuContainer > ul > li:hover > ul{
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−
display: block;
+
−
+
−
}
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−
#menuContainer > ul ul li a {}
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−
#menuContainer > ul ul li a:hover {}
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−
/* Level 3 */
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−
#menuContainer > ul > li > ul > li > ul {
+
−
text-align: left;
+
−
display: none;
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−
background: #1b1c3a;
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−
position: absolute;
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−
left: 100%;
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top: -5px;
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z-index: 9999999;
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}
+
−
#menuContainer > ul > li > ul > li:hover > ul {
+
−
display: block;
+
−
}
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−
#menuContainer > ul ul ul li {}
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−
#menuContainer > ul ul ul li a {}
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−
#menuContainer > ul ul ul li a:hover {}
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#menuContainer.navItem {
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−
                          pointer-events: auto;
+
−
}
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−
                        #Directory { 
+
−
width: 100%;
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overflow:hidden;
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−
float:left;
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−
margin-left:20px;
+
−
                                margin-right: 20px;
+
−
margin-bottom: 0px;
+
−
background-color: #000000;
+
−
border-bottom: 0px solid #565656;
+
−
border-right: 0px solid #565656;
+
−
border-left: 0px solid #565656;
+
−
border-top: 0px solid #565656;
+
−
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
−
}               
+
−
                          /*Style the Directory*/     
+
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                          #Directory table {
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                                Background-color: #000000;
+
−
                                color: #FFFFFF;
+
−
                                margin-left: 0px;
+
−
                                padding: 20px;
+
−
                                line-height: 10px;
+
−
                                height: 100px;
+
−
                                width:100%;
+
−
                                /*float:left;*/
+
−
                          }
+
−
               
+
−
 
+
−
                /*Style the Directory button*/     
+
−
                        #Directory li a{
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−
                                color: #000000;
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−
                                padding: 0px;
+
−
                        }     
+
−
 
+
−
img.button {
+
−
    opacity: 1.0;
+
−
    filter: alpha(opacity=100); /* For IE8 and earlier */
+
−
}
+
−
 
+
−
img.button:hover {
+
−
    opacity: 0.7;
+
−
    filter: alpha(opacity=80); /* For IE8 and earlier */
+
−
}
+
−
 
+
−
 
+
−
/*CLASSES */
+
−
 
+
−
/*Clear class for all the pages, adds spacing too*/
+
−
.clear{
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clear:both;
+
−
height: 10px;
+
−
}
+
−
 
+
−
 
+
−
/* highlight box for special messages */
+
−
    .highlightBox {
+
−
      width:500px;
+
−
                                margin:auto;
+
−
      background-color: #E8E8E9;
+
−
margin-bottom: 15px;
+
−
margin-top: 15px;
+
−
padding: 15px;
+
−
padding-top: 10px;
+
−
    }
+
−
 
+
−
        h1 {
+
−
            font-family: "Abadi MT Condensed Extra Bold", Helvetica, Arial;
+
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            font-size: 30px;
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            font-style: normal;
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            font-variant: normal;
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            font-weight: bold;
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            line-height: 26.4px;
+
−
        }
+
−
        h3 {
+
−
            font-family: "DIN Alternate", Helvetica, Arial;
+
−
            font-size: 18px;
+
−
            font-style: normal;
+
−
            font-variant: normal;
+
−
            font-weight: bold;
+
−
            line-height: 15.4px;
+
−
        }
+
−
        p {
+
−
            font-family: Helvetica, Arial, sans-serif;
+
−
            font-size: 14px;
+
−
            font-style: normal;
+
−
            font-variant: normal;
+
−
            font-weight: light;
+
−
            line-height: 20px;
+
−
        }
+
−
 
+
−
</style>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<!--- THIS IS WHERE THE HTML BEGINS --->
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−
 
+
−
 
+
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<!-- This tells the browser that your page is responsive -->
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−
 
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<head>
+
−
</head>
+
−
 
+
−
 
+
−
<div id="mainContainer">
+
−
  <!--The closing tag for mainContainer should be placed at the bottom of each content page.-->
+
−
  <!-- Start of menu -->
+
−
  <div id="menuContainer">
+
−
    <!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
+
−
    <ul>
+
−
      <li style="padding:0 20px;">
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        <a href="https://igem.org/Main_Page">
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−
          <image src="https://static.igem.org/mediawiki/2015/5/52/HKUST-Rice15_iGEMofficiallogo.jpg" style="width: 82px; height: 72px;  margin: -20px;">
+
−
        </a>
+
−
      </li>
+
−
      <li style="padding:0 20px;">
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−
        <a>
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          <image src="https://static.igem.org/mediawiki/2016/d/d2/Rice_University_seal.svg.png" style="width: 72px; height: 72px;  margin: -20px;">
+
−
        </a>
+
−
      </li>
+
−
      <li><a href="https://2016.igem.org/Team:Rice" style=" margin-top:10px;"><h3>HOME</h3></a></li>
+
−
      <li><a class="navItem" href="https://2016.igem.org/Team:Rice/Description" style=" margin-top:10px;"><h3>PROJECT</h3></a>
+
−
        <ul>
+
−
          <li><a href="https://2016.igem.org/Team:Rice/Description">OVERVIEW</a></li>
+
−
          <li><a href="https://2016.igem.org/Team:Rice/Sofware">SOFTWARE</a>
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            <ul>
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−
              <li><a href="https://2016.igem.org/Team:Rice/Software">inspiration</a></li>
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              <li><a href="https://2016.igem.org/Team:Rice/Software">overview</a></li>
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−
              <li><a href="https://2016.igem.org/Team:Rice/Software">feedback</a></li>
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            </ul>
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          </li>
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          <li><a href="https://2016.igem.org/Team:Rice/Modeling">MODELING</a>
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            <ul>
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              <li><a href="https://2016.igem.org/Team:Rice/Modeling">prediction</a></li>
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              <li><a href="https://2016.igem.org/Team:Rice/Modeling">assumption</a></li>
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              <li><a href="https://2016.igem.org/Team:Rice/Modeling">equations</a></li>
+
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              <li><a href="https://2016.igem.org/Team:Rice/Modeling">references</a></li>
+
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            </ul>
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          </li>
+
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          <li><a href="https://2016.igem.org/Team:Rice/Expression">PARALLEL SENSORS</a>
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            <ul>
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              <li><a href="https://2016.igem.org/Team:Rice/Expression/ParaBAD"><i>P<sub>araBAD</sub></i> characterization</a></li>
+
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            </ul>
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          </li>
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          <li><a href="https://2016.igem.org/Team:Rice/Application">APPLICATION</a></li>
+
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        </ul>
+
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        <li><a class="navItem" href="https://2016.igem.org/Team:Rice/Parts" style=" margin-top:10px;"><h3>ACHIEVEMENT</h3></a>
+
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          <ul>
+
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            <li><a href="https://2016.igem.org/Team:Rice/Parts">PARTS</a></li>
+
−
            <li><a href="https://2016.igem.org/Team:Rice/Medal">MEDAL REQUIREMENTS</a>
+
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              <ul>
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                <li><a href="https://2016.igem.org/Team:Rice/Medal">bronze</a></li>
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                <li><a href="https://2016.igem.org/Team:Rice/Medal">silver</a></li>
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                <li><a href="https://2016.igem.org/Team:Rice/Medal">gold</a></li>
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              </ul>
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            </li>
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          </ul>
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        </li>
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Revision as of 01:32, 28 July 2016


Abstract

Increasing bacterial resistance to classical antibiotics remains a serious threat and urges the development of novel pathogen-killing strategies. Exploiting bacterial communication mechanisms such as quorum sensing is a promising strategy to specifically target certain pathogens. The major aim of this project is the introduction of a genetic circuit enabling Bacillus subtilis to actively detect, attach to, and eventually kill Staphylococcus aureus and Streptococcus pneumoniae.

Initially, we will introduce the autoinducer-sensing two-component systems of S. aureus and S. pneumoniae into B. subtilis. to create a pathogen-detecting strain. By utilizing quorum sensing-dependent promoters, we will then trigger pathogen-killing strategies like the production of antimicrobial peptides or biofilm degradation. As a safety measure a delayed suicide-switch guarantees non-persistence of genetically modified B. subtilis in the absence of pathogens. We envision the use of BaKillus as a smart, cheap and simple-to-use medical device for diagnostics and targeted treatment of multiresistant super bugs.