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− | < | + | <h>Transformation </h> |
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− | < | + | <ol>1. Thaw competent cells rapidly by immersing frozen tubes in a 37°C water bath</ol> |
− | + | ||
− | < | + | <ol>2. Immediately, add one volume of SpII + EGTA to the Thawed cells; mix gently </ol> |
− | < | + | |
+ | <ol>3. In a sterile test tube add competent cell (0.2~0.5 ml) to the DNA solution (<0.1 ml) and incubate in a roller drum at 37.</ol> | ||
+ | |||
+ | <ol>4. Dilute the transformed cells as appropriate in T base containing 0.5% glucose and plate immediately onto selective media. </ol> | ||
+ | </div></a></li></ul></li> | ||
Revision as of 14:19, 29 July 2016