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<p align="center"><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Transfo_pic_1.png" width="600px"/></p> | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Transfo_pic_1.png" width="600px"/></p> | ||
+ | |||
+ | <h>Digestion</h> | ||
+ | <ol>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. | ||
+ | In a 1.5mL tube combine the following: | ||
+ | <ul> DNA </ul> | ||
+ | <ul>Restriction Enzyme(s)</ul> | ||
+ | <ul>Buffer </ul> | ||
+ | <ul>dH2O up to total volume</ul> | ||
+ | <p>Mix gently by pipetting. </p></ol> | ||
+ | |||
+ | <ol>2. Incubate tube at appropriate temperature (usually 37°C) for 1 hour. </ol> | ||
+ | |||
+ | <ol>3. Always follow the manufacturer’s instructions. </ol> | ||
+ | |||
+ | <ol>4. To visualize the results of your digest, conduct gel electrophoresis</ol> | ||
+ | |||
</div></a></li></ul></li> | </div></a></li></ul></li> | ||
Revision as of 14:29, 29 July 2016